AQP3 抗体 (C-Term)
(1 validation)-
- 抗原 See all AQP3 抗体
- AQP3 (Aquaporin 3 (Gill Blood Group) (AQP3))
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抗原表位
- C-Term
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适用
- 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This AQP3 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
- 特异性
- Detects ~31.5 kDa. May detect larger glycosylated bands ~35-50 kDa.
- 交叉反应
- 人, 小鼠, 大鼠
- 纯化方法
- Protein A Purified
- 免疫原
- Produced against the C-terminal peptide (Sequence N-CHLEQPPPSTEAENVKLAHMKHKEQI) of rat aquaporin 3
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anti-Aquaporin 3 (Gill Blood Group) (AQP3) (AA 178-292) antibody
AQP3 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Aquaporin 3 (Gill Blood Group) (AQP3) (AA 278-292), (C-Term) antibodyAQP3 适用: 人, 小鼠, 大鼠 WB, IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Aquaporin 3 (Gill Blood Group) (AQP3) (AA 201-292) antibodyAQP3 适用: 人, 小鼠, 大鼠 WB, ELISA, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Aquaporin 3 (Gill Blood Group) (AQP3) (AA 210-244) antibodyAQP3 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Aquaporin 3 (Gill Blood Group) (AQP3) (AA 163-191) antibodyAQP3 适用: 人, 小鼠 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Aquaporin 3 (Gill Blood Group) (AQP3) (AA 268-292) antibodyAQP3 适用: 人, 小鼠, 大鼠 WB, IHC, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Aquaporin 3 (Gill Blood Group) (AQP3) (C-Term) antibody (HRP)AQP3 适用: 大鼠 WB, IHC, IF, ICC 宿主: 兔 Polyclonal HRP
anti-Aquaporin 3 (Gill Blood Group) (AQP3) (C-Term) antibody (FITC)AQP3 适用: 大鼠 WB, IHC, IF, ICC 宿主: 兔 Polyclonal FITC
anti-Aquaporin 3 (Gill Blood Group) (AQP3) (C-Term) antibody (Biotin)AQP3 适用: 大鼠 WB, IHC, IF, ICC 宿主: 兔 Polyclonal Biotin
anti-Aquaporin 3 (Gill Blood Group) (AQP3) (C-Term) antibody (PE)AQP3 适用: 大鼠 WB, IHC, IF, ICC 宿主: 兔 Polyclonal PE
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- 应用备注
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- WB (1:2000)
- IHC (1:200)
- ICC/IF (1:400)
- optimal dilutions for assays should be determined by the user.
- 说明
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0.5 μg/ml of ABIN863208 was sufficient for detection of aquaporin 3 in 10 μg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
- 限制
- 仅限研究用
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- by
- Centre de Recherche en Transplantation et Immunologie, UMR1064, Université de Nantes
- No.
- #102635
- 日期
- 2018.12.14
- 抗原
- AQP3
- Lot Number
- 160308
- Method validated
- Immunofluorescence
- Positive Control
Membrane staining in compacted morula; membrane staining apical side, on early blastocysts
- Negative Control
Cells not expressing AQP3 mRNA within human embryos (inner cell mass)
No primary control
- Notes
Passed. ABIN863208 specifically labels the targeted antigen in embryos in IF. No signal was detected in sample negative control tissue and the secondary antibody only control.
- Primary Antibody
- ABIN863208
- Secondary Antibody
- donkey anti-rabbit antibody (Life Technologies, A21206)
- Full Protocol
- Fixation
- Fix embryos in one drop of 30µl of 4% paraformaldehyde for 5min at RT.
- Remove embryos and wash quickly three times with PBS containing 0.1% BSA (in three drops of 30µl).
- Staining:
- Permeabilize embryo in 300µl IF buffer for 60min at RT.
- Incubate embryo with the primary rabbit anti-AQP3 antibody (antibodies-online, ABIN863208, 160308) diluted 1:400 in buffer ON at 4°C.
- Rinse embryo in IF buffer quickly three times.
- Incubate embryo with secondary donkey anti-rabbit antibody (Life Technologies, A21206) diluted 1:1000 in buffer for 2h at RT.
- Rinse embryo in PBS-BSA.
- At this point, the embryos can either be kept in PBS-BSA ON at 4°C or used for imaging directly on ibido µ-Slide.
- Image acquisition on a microscope using appropriate filters, 20x oil-immersion objective.
- Experimental Notes
Labeling of human embryo with the aquaporin 3 antibody ABIN863208 shows the expected staining pattern membrane-associated outside of the blastocyst. The background staining in our conditions is a bit high and might be improved with more stringent washes or different fixation-permeabilization protocols.
生效 #102635 (Immunofluorescence)!['独立验证'标志]( "成功验证")
!['独立验证'标志]( "成功验证")
Validation Images![Immunoflurescence analysis of human embryo using ABIN863208. AQP3 (right) with nuclear counterstaining (left) at indicated stages. In the top panel (morula), the staining is located at the membrane. In the bottom panel (early blastocyst), the staining is located at the membrane, in trophectoderm cells. Scale bar = 47µm.]()
Immunoflurescence analysis of human embryo using ABIN863208. AQP3 (right) with nuclear counterstaining (left) at indicated stages. In the top panel (morula), the staining is located at the membrane. In the bottom panel (early blastocyst), the staining is located at the membrane, in trophectoderm cells. Scale bar = 47µm.
Full Methods -
- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- -20°C
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- 抗原
- AQP3 (Aquaporin 3 (Gill Blood Group) (AQP3))
- 别名
- Aquaporin 3 (AQP3 产品)
- 别名
- aqp3 antibody, wu:fa95h06 antibody, wu:fi13e05 antibody, zgc:55525 antibody, Tb06.28P18.240 antibody, AQP-2 antibody, AQP-3 antibody, GIL antibody, SAQP3 antibody, aquaporin 3a antibody, aquaporin 3 (Gill blood group) antibody, aquaporin 3 antibody, aqp3a antibody, AQP3 antibody, Tb927.6.1520 antibody, MCYG_07750 antibody, Aqp3 antibody
- 背景
- Aquaporins selectively conduct water molecules in and out of the cell, while preventing the passage of ions and other solutes. Known as water channels, they are integral membrane pore proteins (1, 2). Aquaporin 3 is found in the basolateral cell membrane of principal collecting duct cells and provide a pathway for water to exit these cells (3). AQP3 gene expression is not regulated by vasopressin (4).
- 基因ID
- 65133
- NCBI登录号
- NP_113891
- UniProt
- P47862
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