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- 抗原 See all SLC9A3 抗体
- SLC9A3 (Solute Carrier Family 9 (Sodium/hydrogen Exchanger), Member 3 (SLC9A3))
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适用
- 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This SLC9A3 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)
- 特异性
- Most abundant in colon and small intestine, followed by kidney and stomach. In kidney, expressed in proximal tubules and outer medulla (at protein level),Detects ~84 kDa.
- 交叉反应
- 小鼠, 大鼠
- 纯化方法
- Protein A Purified
- 免疫原
- Synthetic peptide mapping to AA 809 to 831 of rat sequence
- Top Product
- Discover our top product SLC9A3 Primary Antibody
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- 应用备注
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- WB (1:1000)
- IHC (1:25)
- optimal dilutions for assays should be determined by the user.
- 说明
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1 μg/ml of ABIN863199 was sufficient for detection of HNE3 in 10 μg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- -20°C
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ER stress and basement membrane defects combine to cause glomerular and tubular renal disease resulting from Col4a1 mutations in mice." in: Disease models & mechanisms, Vol. 9, Issue 2, pp. 165-76, (2016) (PubMed).
: "Effect of dietary protein restriction on renal ammonia metabolism." in: American journal of physiology. Renal physiology, Vol. 308, Issue 12, pp. F1463-73, (2015) (PubMed).
: "Haploinsufficiency of the ammonia transporter Rhcg predisposes to chronic acidosis: Rhcg is critical for apical and basolateral ammonia transport in the mouse collecting duct." in: The Journal of biological chemistry, Vol. 288, Issue 8, pp. 5518-29, (2013) (PubMed).
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ER stress and basement membrane defects combine to cause glomerular and tubular renal disease resulting from Col4a1 mutations in mice." in: Disease models & mechanisms, Vol. 9, Issue 2, pp. 165-76, (2016) (PubMed).
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- 抗原
- SLC9A3 (Solute Carrier Family 9 (Sodium/hydrogen Exchanger), Member 3 (SLC9A3))
- 别名
- NHE3 (SLC9A3 产品)
- 别名
- SLC9A3 antibody, 9030624O13Rik antibody, AI930210 antibody, NHE-3 antibody, NHE3 antibody, Nhe3 antibody, solute carrier family 9 member A3 antibody, sodium/hydrogen exchanger 3 antibody, Sodium/hydrogen exchanger 3 antibody, putative sodium/hydrogen exchanger 3 (nhe3) antibody, solute carrier family 9 (sodium/hydrogen exchanger), member 3 antibody, SLC9A3 antibody, LOC5570922 antibody, GL50803_102647 antibody, Smp_045680 antibody, Slc9a3 antibody
- 背景
- Sodium-hydrogen exchanger 3 (NHE3, Slc9a3) is an epithelial transport protein that carries out 1:1 exchange of Na+ and H+ across the plasma membrane. It is apically located in the proximal tubule of the kidney, the thick ascending limb of the kidney, and in small intestine (1). NHE3 is phosphorylated and regulated by multiple kinases including PKA, SGK1 and CK2. It can be phosphorylated by calyculin A, and dephosphorylated by PP1 catalytic subunit in vitro (2).
- 基因ID
- 24784
- NCBI登录号
- NP_036786
- UniProt
- P26433
- 途径
- Proton Transport
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