KDEL 抗体
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- 抗原 See all KDEL (Lys Asp Glu Leu) products
- KDEL (Lys Asp Glu Leu)
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适用
- 小鼠, 大鼠, 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This KDEL antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)
- 特异性
- Detects KDEL proteins, GRP94, Grp78, PDI and calreticulin. It may also see ERp57 and ERp29.
- 纯化方法
- Protein A Purified
- 免疫原
- KDEL containing peptide immunogen
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anti-KDEL (Lys Asp Glu Leu) (AA 649-654) antibody
Lys Asp Glu Leu 适用: Avian, Insect, 哺乳动物, 植物, Saccharomyces cerevisiae WB, FACS, ICC, IF, IHC (p), ELISA, EM, IP 宿主: 小鼠 Monoclonal 10C3 unconjugated
anti-KDEL (Lys Asp Glu Leu) antibody (HRP)Lys Asp Glu Leu 适用: 小鼠, 大鼠, 人 WB, IHC, ICC, IF 宿主: 兔 Polyclonal HRP
anti-KDEL (Lys Asp Glu Leu) antibody (FITC)Lys Asp Glu Leu 适用: 小鼠, 大鼠, 人 WB, IHC, ICC, IF 宿主: 兔 Polyclonal FITC
anti-KDEL (Lys Asp Glu Leu) antibody (Alkaline Phosphatase (AP))Lys Asp Glu Leu 适用: 小鼠, 大鼠, 人 WB, IHC, ICC, IF 宿主: 兔 Polyclonal Alkaline Phosphatase (AP)
anti-KDEL (Lys Asp Glu Leu) antibody (Biotin)Lys Asp Glu Leu 适用: 小鼠, 大鼠, 人 WB, IHC, ICC, IF 宿主: 兔 Polyclonal Biotin
anti-KDEL (Lys Asp Glu Leu) antibody (PE)Lys Asp Glu Leu 适用: 小鼠, 大鼠, 人 WB, IHC, ICC, IF 宿主: 兔 Polyclonal PE
anti-KDEL (Lys Asp Glu Leu) antibody (APC)Lys Asp Glu Leu 适用: 小鼠, 大鼠, 人 WB, IHC, ICC, IF 宿主: 兔 Polyclonal APC
anti-KDEL (Lys Asp Glu Leu) antibody (PerCP)Lys Asp Glu Leu 适用: 小鼠, 大鼠, 人 WB, IHC, ICC, IF 宿主: 兔 Polyclonal PerCP
anti-KDEL (Lys Asp Glu Leu) antibody (Atto 488)Lys Asp Glu Leu 适用: 小鼠, 大鼠, 人 WB, IHC, ICC, IF 宿主: 兔 Polyclonal Atto 488
anti-KDEL (Lys Asp Glu Leu) antibody (Atto 594)Lys Asp Glu Leu 适用: 小鼠, 大鼠, 人 WB, IHC, ICC, IF 宿主: 兔 Polyclonal Atto 594
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- 应用备注
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- WB (1:1000)
- ICC/IF (1:100)
- optimal dilutions for assays should be determined by the user.
- 说明
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A 1:1000 dilution of ABIN863187 was sufficient for detection of KDEL-containing proteins in 20 μg of HeLa cell lysate by ECL immunoblot analysis using goat anti-mouse IgG as the secondary.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- PBS pH 7.2, 50 % glycerol, 0.09 % sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- 储存条件
- -20 °C
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- 抗原
- KDEL (Lys Asp Glu Leu)
- Abstract
- Lys Asp Glu Leu 产品
- 背景
- The endoplasmic reticulum is part of a protein sorting pathway, or in essence, the transportation system of the eukaryotic cell. The majority of endoplasmic reticulum resident proteins are retained in the endoplasmic reticulum through a retention motif. This motif is composed of four amino acids at the C-terminal end of the protein sequence. The most common retention sequence is KDEL (lys-asp-glu-leu). Grp78 and Grp94 and PDI all share the C-terminal KDEL sequence. The presence of carboxy-terminal KDEL appears to be necessary for ER retention and appears to be sufficient to reduce the secretion of proteins from the ER.
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