Piccolo 抗体
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- 抗原 See all Piccolo (PIC) products
- Piccolo (PIC)
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适用
- 小鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Piccolo antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)
- 特异性
- Detects ~550 kDa. No cross-reactivity to Bassoon.
- 交叉反应
- 人, 小鼠, 大鼠
- 纯化方法
- Protein G Purified
- 免疫原
- Full length recombinant protein (GenBank ID: BC001304.1)
- 克隆位点
- 6H9-B6
- 亚型
- IgG2b kappa
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- 应用备注
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- WB (1:1000)
- ICC/IF (1:100)
- optimal dilutions for assays should be determined by the user.
- 说明
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1 μg/ml of ABIN863104 was sufficient for detection of Piccolo in 20 μg of rat brain tissue lysate by colorimetric immunoblot analysis using goat IgG:HRP as the secondary antibody.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- -20°C
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- 抗原
- Piccolo (PIC)
- 别名
- Piccolo (PIC 产品)
- 背景
- Piccolo, also referred to as Aczonin, is a large protein which consists of an N-terminal Zn2+ finger, several piccolo-bassoon homology domains and C-terminal PDZ and C2 domains (1, 2). In general it is found together with bassoon, a related huge multi-domain protein of the CAZ (cytoskeletal matric assembled at active zones) (3). Piccolo is a scaffolding protein for proteins involved in endo- and exocytosis of synaptic vesicles. Piccolo has also been shown to interfere with clathrin medicated endocytosis by binding to the F-actin and dynamin binding protein Abp1 (2).
- 基因ID
- 26875
- NCBI登录号
- NP_001104266
- UniProt
- Q9QYX7
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