LY75/DEC-205 抗体 (PE)
(1 reference)-
- 抗原 See all LY75/DEC-205 (LY75) 抗体
- LY75/DEC-205 (LY75) (Lymphocyte Antigen 75 (LY75))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This LY75/DEC-205 antibody is conjugated to PE
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应用范围
- Flow Cytometry (FACS)
- 特异性
- The mouse monoclonal antibody HD30 recognizes an extracellular epitope of CD205, an approx. 200 kDa C-type lectin transmembrane protein of the MMR (macrophage mannose receptor) family, expressed at high levels on dendritic cells and thymic epithelial cells, and at low levels on lymphocytes, NK cells and monocytes.
- 交叉反应 （详细）
- Human
- 纯化方法
- Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
- 免疫原
- Recombinant Fc-tagged human CD205
- 克隆位点
- HD30
- 亚型
- IgG1 kappa
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anti-Lymphocyte Antigen 75 (LY75) antibody
LY75 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 4B10E11 unconjugated
anti-Lymphocyte Antigen 75 (LY75) (AA 440-680) antibodyLY75 适用: 人 ELISA, WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Lymphocyte Antigen 75 (LY75) antibodyLY75 适用: 人 WB, FACS, IHC (fro) 宿主: 小鼠 Monoclonal HD30 unconjugated
anti-Lymphocyte Antigen 75 (LY75) (AA 1520-1666) antibodyLY75 适用: 人 ELISA 宿主: 小鼠 Monoclonal 3A4A10 unconjugated
anti-Lymphocyte Antigen 75 (LY75) (C-Term) antibodyLY75 适用: 人 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Lymphocyte Antigen 75 (LY75) (Internal Region) antibodyLY75 适用: 人, 小鼠 ELISA, WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Lymphocyte Antigen 75 (LY75) antibody (PE)LY75 适用: Cow, 绵羊 FACS 宿主: 小鼠 Monoclonal IL-A114 PE
anti-Lymphocyte Antigen 75 (LY75) antibody (APC)LY75 适用: 人 FACS 宿主: 小鼠 Monoclonal HD30 APC
anti-Lymphocyte Antigen 75 (LY75) (AA 646-675) antibodyLY75 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB23558 unconjugated
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- 应用备注
- Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
- 说明
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The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
- 限制
- 仅限研究用
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- 溶解方式
- No reconstitution is necessary.
- 缓冲液
- Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
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Do not freeze.
Avoid prolonged exposure to light. - 储存条件
- 4 °C
- 储存方法
- Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
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: "Generation of anti-human DEC205/CD205 monoclonal antibodies that recognize epitopes conserved in different mammals." in: Journal of immunological methods, Vol. 377, Issue 1-2, pp. 15-22, (2012) (PubMed).
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: "Generation of anti-human DEC205/CD205 monoclonal antibodies that recognize epitopes conserved in different mammals." in: Journal of immunological methods, Vol. 377, Issue 1-2, pp. 15-22, (2012) (PubMed).
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- 抗原
- LY75/DEC-205 (LY75) (Lymphocyte Antigen 75 (LY75))
- 别名
- CD205 (LY75 产品)
- 别名
- LY75 antibody, MGC140308 antibody, DEC205 antibody, im:7150926 antibody, si:dkey-78p20.2 antibody, LYT75 antibody, CD205 antibody, DEC-205 antibody, CLEC13B antibody, GP200-MR6 antibody, LY-75 antibody, RGD1565962 antibody, Ly-75 antibody, lymphocyte antigen 75 antibody, CD205 protein antibody, CD302 antigen antibody, LY75 antibody, Cyan7822_2917 antibody, LY5 antibody, ly75 antibody, cd205 antibody, LOC100380704 antibody, LOC100433861 antibody, LOC100483541 antibody, Ly75 antibody
- 背景
- Lymphocyte antigen 75,CD205, also known as DEC-205, is an endocytic receptor of macrophage mannose receptor family. This 205 kDa C-type lectin transmembrane protein mediates adsorptive uptake and its intracellular domain contains coated pit localization sequence and distal acidic motif, which is required for recycling beyond early endosomes through deeper MHC II+ late endosomes and lysosomes. This unique pathway of receptor-mediated uptake proves to be necessary for presentation of antigenic peptides at low doses of ligand. CD205 is responsible for uptake and processing of captured antigens for dendritic cells.,DEC-205, LY75, CLEC13B
- 基因ID
- 4065
- UniProt
- O60449
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