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Notch1 抗体 (PE)

Name: Notch1 抗体 (PE)
Brand: antibodies-online
SKU: ABIN807924
Availability: OutOfStock

NOTCH1 适用: 人, 小鼠 FACS 宿主: 小鼠 Monoclonal mN1A PE

Flow cytometry intracellular staining patterns of PHA stimulated human peripheral whole blood stained using anti-Notch1 (mN1A) PE antibody (concentration in sample 3 μg/mL, left) or mouse IgG1 isotype control (MOPC-21) PE antibody (concentration in sample 3 μg/mL, same as Notch1 PE concentration, right). (Notch1 抗体 (PE))

FACS

Intracellular staining of Notch1 in Jurkat cells using anti-Notch1 (mN1A) PE. (Notch1 抗体 (PE))

FACS

Separation of CD3 positive CD25 positive cells stained using anti-Notch1 (mN1A) PE antibody (concentration in sample 3 μg/mL, red-filled) from CD3 positive CD25 positive cells stained using mouse IgG1 isotype control (MOPC-21) PE antibody (concentration in sample 3 μg/mL, same as Notch1 PE concentration, black-dashed) in flow cytometry analysis (intracellular staining) of PHA stimulated human peripheral whole blood . (Notch1 抗体 (PE))

FACS

Flow cytometry multicolor surface staining pattern of human PHA stimulated CD3 positive lymphocytes using anti-human CD25 (MEM-181) APC antibody (10 μL reagent / 100 μL of sample) and intracellular staining of human lymphocytes using anti-Notch1 (mN1A) PE antibody (concentration in sample 3 μg/mL). (Notch1 抗体 (PE))

FACS

4 images

(6 references)

产品编号 ABIN807924

发货至: 中国

Datasheet as PDF

抗原 See all Notch1 (NOTCH1) 抗体

Notch1 (NOTCH1) (Notch 1 (NOTCH1))
适用
120

87

49

10

3

3

1

1

1

1

1

1

1

1
人, 小鼠
宿主
99

36

6

3

2

1
小鼠
Compatible Secondaries
克隆类型
95

48

2
单克隆
标记
67

10

9

7

6

6

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1
This Notch1 antibody is conjugated to PE
应用范围
95

46

46

31

27

26

18

17

16

10

10

4

3

1

1

1

1

1
Flow Cytometry (FACS)

特异性

The mouse monoclonal antibody mN1A recognizes intracellular domain of Notch 1 protein, mainly its activated form. The unprocessed Notch 1 protein is recognized with lower affinity.

无交叉反应

大鼠

交叉反应（详细）

Human, Mouse

纯化方法

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

免疫原

GST fusion protein containing cdc10-NCR region of mouse Notch1

克隆位点

MN1A

亚型

IgG1 kappa
anti-Notch 1 (NOTCH1) antibody (APC)
NOTCH1 适用: 人, 小鼠 FACS 宿主: 小鼠 Monoclonal mN1A APC

anti-Notch 1 (NOTCH1) (AA 2101-2300) antibody
NOTCH1 适用: 人, 小鼠, 大鼠 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Notch 1 (NOTCH1) (AA 20-216) antibody
NOTCH1 适用: 小鼠 WB, IF, ICC 宿主: 小鼠 Monoclonal S253-32 unconjugated

anti-Notch 1 (NOTCH1) (AA 20-216) antibody (HRP)
NOTCH1 适用: 小鼠 WB, IF, ICC 宿主: 小鼠 Monoclonal S253-32 HRP

anti-Notch 1 (NOTCH1) (AA 20-216) antibody (FITC)
NOTCH1 适用: 小鼠 WB, IF, ICC 宿主: 小鼠 Monoclonal S253-32 FITC

anti-Notch 1 (NOTCH1) (AA 20-216) antibody (Biotin)
NOTCH1 适用: 小鼠 WB, IF, ICC 宿主: 小鼠 Monoclonal S253-32 Biotin

anti-Notch 1 (NOTCH1) (AA 20-216) antibody (PE)
NOTCH1 适用: 小鼠 WB, IF, ICC 宿主: 小鼠 Monoclonal S253-32 PE

anti-Notch 1 (NOTCH1) (AA 20-216) antibody (APC)
NOTCH1 适用: 小鼠 WB, IF, ICC 宿主: 小鼠 Monoclonal S253-32 APC

anti-Notch 1 (NOTCH1) (AA 20-216) antibody (PerCP)
NOTCH1 适用: 小鼠 WB, IF, ICC 宿主: 小鼠 Monoclonal S253-32 PerCP

anti-Notch 1 (NOTCH1) (AA 20-216) antibody (Atto 488)
NOTCH1 适用: 小鼠 WB, IF, ICC 宿主: 小鼠 Monoclonal S253-32 Atto 488

应用备注

Flow cytometry: Recommended dilution: 1-5 μg/mL. Intracellular staining.

说明

The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.

限制

仅限研究用
浓度

0.1 mg/mL

缓冲液

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Do not freeze.
Avoid prolonged exposure to light.

储存条件

4 °C

储存方法

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Khwaja, Liu, Tong, Jin, Pear, van Deursen, Bram: "HIV-1 Rev-binding protein accelerates cellular uptake of iron to drive Notch-induced T cell leukemogenesis in mice." in: The Journal of clinical investigation, Vol. 120, Issue 7, pp. 2537-48, (2010) (PubMed).

Kefas, Comeau, Floyd, Seleverstov, Godlewski, Schmittgen, Jiang, diPierro, Li, Chiocca, Lee, Fine, Abounader, Lawler, Purow: "The neuronal microRNA miR-326 acts in a feedback loop with notch and has therapeutic potential against brain tumors." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 29, Issue 48, pp. 15161-8, (2009) (PubMed).

Watanabe, Nagaoka, Lee, Bianco, Gonzales, Castro, Rangel, Sakamoto, Sun, Callahan, Salomon: "Enhancement of Notch receptor maturation and signaling sensitivity by Cripto-1." in: The Journal of cell biology, Vol. 187, Issue 3, pp. 343-53, (2009) (PubMed).

Tagami, Okochi, Yanagida, Ikuta, Fukumori, Matsumoto, Ishizuka-Katsura, Nakayama, Itoh, Jiang, Nishitomi, Kamino, Morihara, Hashimoto, Tanaka, Kudo, Chiba, Takeda: "Regulation of Notch signaling by dynamic changes in the precision of S3 cleavage of Notch-1." in: Molecular and cellular biology, Vol. 28, Issue 1, pp. 165-76, (2007) (PubMed).

Sun, Li, Vercherat, Gulbagci, Acharjee, Li, Chung, Thin, Taneja: "Stra13 regulates satellite cell activation by antagonizing Notch signaling." in: The Journal of cell biology, Vol. 177, Issue 4, pp. 647-57, (2007) (PubMed).

Huppert, Le, Schroeter, Mumm, Saxena, Milner, Kopan: "Embryonic lethality in mice homozygous for a processing-deficient allele of Notch1." in: Nature, Vol. 405, Issue 6789, pp. 966-70, (2000) (PubMed).
抗原

Notch1 (NOTCH1) (Notch 1 (NOTCH1))

别名

Notch 1 (NOTCH1 产品)

别名

notch1 antibody, NOTCH1 antibody, hn1 antibody, tan1 antibody, notch antibody, xotch antibody, xnotch antibody, notch-1 antibody, xnotch1 antibody, TAN1 antibody, hN1 antibody, 9930111A19Rik antibody, Mis6 antibody, N1 antibody, Tan1 antibody, lin-12 antibody, NOTCH antibody, Xotch antibody, notch1-a antibody, notch 1 antibody, fibropellin-1 antibody, Notch homolog 1, translocation-associated (Drosophila) antibody, Notch homolog 1, translocation-associated antibody, notch 1 S homeolog antibody, notch1 antibody, LOC100631526 antibody, NOTCH1 antibody, Notch1 antibody, notch1.S antibody

背景

Notch receptor 1,Notch 1 is a 270-300 kDa transmembrane heterodimeric protein with multiple extracellular growth factor-like repeats, and with an intracellular domain consisting of multiple different domain types. It serves as a receptor for membrane ligands, such as Delta 1, Jagged 1 (CD339), and Jagged 2, and regulates cell fate decisions. Upon ligand binding the transmembrane form of Notch 1 is repeatedly cleaved to provide approximately 120 kDa Notch intracellular fragment (NICD), which translocates to the nucleus and acts as a part of transcriptional complexes that alter differentiation, proliferation, and apoptosis. The highest level of Notch 1 expression is in brain, lung and thymus.,AOS5, TAN1, hN1, AOVD1

基因ID

4851

UniProt

P46531

途径

Notch Signaling, Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development