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CD4 抗体 (Biotin)

CD4 适用: 人 FACS 宿主: 小鼠 Monoclonal 7E14 Biotin
产品编号 ABIN793379
发货至: 中国
  • 抗原 See all CD4 抗体
    CD4
    适用
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    宿主
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    小鼠
    克隆类型
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    单克隆
    标记
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    This CD4 antibody is conjugated to Biotin
    应用范围
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    Flow Cytometry (FACS)
    特异性
    Human CD4
    纯化方法
    Protein A/G purified
    过滤
    Sterile filtered
    免疫原
    Human peripheral blood T lymphocytes.
    克隆位点
    7E14
    亚型
    IgG1
    Top Product
    Discover our top product CD4 Primary Antibody
  • 应用备注
    Approved: Flo

    Usage: PBMC: Add 10 μL of antibody/10^6 PBMC in 100 μL PBS. Mix gently and incubate for 15 minutes at 2° to 8 °C. Wash twice with PBS and analyze or fix with 0.5 % v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 μL of antibody/100 μL of whole blood. Mix gently and incubate for 15 minutes at room temperature 20 °C. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5 % v/v of paraformaldehyde in PBS and analyze. See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. ALLOPHYCOCYANIN: (APC) conjugates are analyzed in multi-color flow cytometry with instruments equipped with a second laser and proper filters. Laser excitation is at 633 nm with a Helium Neon (HeNe) laser or a 600-640 nm (633 nm) range for a Dye laser. Peak fluorescence emission is at 660 nm. RPE-Cy-5 +: Use 5 μL of solution, rather than 10 μL as above. Excites at 488nm and emits at 670nm. Store protected from light. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
    说明

    Target Species of Antibody: Human

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    Lot specific
    缓冲液
    PBS, 0.08 % sodium azide, 0.2 % carrier protein, sterile-filtered
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Do not freeze.
    储存条件
    4 °C
    储存方法
    Store at 4°C. Do not freeze.
  • 抗原
    CD4
    别名
    CD4 (CD4 产品)
    别名
    cd4 antibody, L3T4 antibody, Ly-4 antibody, CD4mut antibody, W3/25 antibody, p55 antibody, CD4 molecule antibody, carcinoembryonic antigen-related cell adhesion molecule 5 antibody, T-cell surface glycoprotein CD4 antibody, CD4 antigen antibody, Cd4 molecule antibody, CD4 antibody, LOC100125525 antibody, cd4 antibody, Cd4 antibody
    背景
    Name/Gene ID: CD4

    Synonyms: CD4, CD4 antigen, CD4 receptor, CD4mut, CD4 antigen (p55), CD4 Molecule
    基因ID
    920
    UniProt
    P01730
    途径
    TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events
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