CD4 抗体 (Biotin)
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- 抗原 See all CD4 抗体
- CD4
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CD4 antibody is conjugated to Biotin
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应用范围
- Flow Cytometry (FACS)
- 特异性
- Human CD4
- 纯化方法
- Protein A/G purified
- 过滤
- Sterile filtered
- 免疫原
- Human peripheral blood T lymphocytes.
- 克隆位点
- 7E14
- 亚型
- IgG1
- Top Product
- Discover our top product CD4 Primary Antibody
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- 应用备注
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Approved: Flo
Usage: PBMC: Add 10 μL of antibody/10^6 PBMC in 100 μL PBS. Mix gently and incubate for 15 minutes at 2° to 8 °C. Wash twice with PBS and analyze or fix with 0.5 % v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 μL of antibody/100 μL of whole blood. Mix gently and incubate for 15 minutes at room temperature 20 °C. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5 % v/v of paraformaldehyde in PBS and analyze. See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. ALLOPHYCOCYANIN: (APC) conjugates are analyzed in multi-color flow cytometry with instruments equipped with a second laser and proper filters. Laser excitation is at 633 nm with a Helium Neon (HeNe) laser or a 600-640 nm (633 nm) range for a Dye laser. Peak fluorescence emission is at 660 nm. RPE-Cy-5 +: Use 5 μL of solution, rather than 10 μL as above. Excites at 488nm and emits at 670nm. Store protected from light. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. - 说明
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Target Species of Antibody: Human
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, 0.08 % sodium azide, 0.2 % carrier protein, sterile-filtered
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Do not freeze.
- 储存条件
- 4 °C
- 储存方法
- Store at 4°C. Do not freeze.
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- 抗原
- CD4
- 别名
- CD4 (CD4 产品)
- 别名
- cd4 antibody, L3T4 antibody, Ly-4 antibody, CD4mut antibody, W3/25 antibody, p55 antibody, CD4 molecule antibody, carcinoembryonic antigen-related cell adhesion molecule 5 antibody, T-cell surface glycoprotein CD4 antibody, CD4 antigen antibody, Cd4 molecule antibody, CD4 antibody, LOC100125525 antibody, cd4 antibody, Cd4 antibody
- 背景
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Name/Gene ID: CD4
Synonyms: CD4, CD4 antigen, CD4 receptor, CD4mut, CD4 antigen (p55), CD4 Molecule - 基因ID
- 920
- UniProt
- P01730
- 途径
- TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events
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