Albumin 抗体 (FITC)
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- 抗原 See all Albumin (ALB) 抗体
- Albumin (ALB)
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适用
- 小鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Albumin antibody is conjugated to FITC
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应用范围
- Immunofluorescence (IF), Immunodiffusion (ID), Radioimmunoassay (RIA)
- 特异性
- The defined antibody specificity is directed to Albumin as tested against Mouse sera. In Immunoelectrophoresis and double radial Immunodiffusion (Ouchterlony), using various antiserum concentrations against appropriate concentrations of the immunogen, a single characteristic precipitin line is obtained which shows a reaction of identity with the precipitin lines obtained against Mouse serum and the purified Albumin. Cross-reactivity: Inter-species cross-reactivity is a normal feature of antibodies to serum proteins, since homologous proteins of different species frequently share antigenic determinants. The degree of cross-reactivity is also dependent on the concentrations of the reactants and the sensitivity of the assay arrangement. This antiserum has been tested for cross-reactivity in Double Radial Immunodiffusion with the following results: Bovine (-), Chicken (-), Canine (-), Goat (-), Guinea Pig (++), Hamster (+), Horse (+), Human (+), Monkey (+), Rat (++), Sheep (-), Swine (+).
- 交叉反应 (详细)
- Species reactivity (tested):Mouse.
- 产品特性
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Absorption / Emission: 492 nm / 515 nm
Molar Ratio: Fluorescein/IgG ~1.7 - 纯化方法
- Hyperimmune with strong precipitating activity are selected for fractionation by Salt Precipitation and the purification of the IgG fraction by DEAE Chromatography
- 免疫原
- Albumin is isolated from Mouse serum by Sequential Precipitation and purified by Ion Exchange Chromatography and Affinity Chromatography. Freund’s complete adjuvant is used in the first step of the immunization procedure.
- 亚型
- IgG
- Top Product
- Discover our top product ALB Primary Antibody
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 溶解方式
- Restore by adding 1 mL of sterile distilled water
- 浓度
- 10.0 mg/mL
- 缓冲液
- PBS, pH 7.2 without preservatives and foreign proteins
- 储存液
- Without preservative
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one week or (in aliquots) at -20 °C for longer.
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- 抗原
- Albumin (ALB)
- 别名
- Albumin (ALB 产品)
- 背景
- Albumin is a soluble, monomeric protein which comprises about one half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Mutations in this gene on chromosome 4 result in various anomalous proteins. Albumin is a globular unglycosylated serum protein of molecular weight 65,000. The human albumin gene is 16,961 nucleotides long from the putative 'cap' site to the first poly(A) addition site. It is split into 15 exons which are symmetrically placed within the 3 domains that are thought to have arisen by triplication of a single primordial domain. Albumin is synthesized in the liver as preproalbumin which has an N terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin.Synonyms: ALB, BSA, HSA, Serum Albumin
- 基因ID
- 11657
- NCBI登录号
- NP_033784
- 途径
- Lipid Metabolism
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