SIRT3 抗体 (Internal Region)
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- 抗原 See all SIRT3 抗体
- SIRT3 (Sirtuin 3 (SIRT3))
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抗原表位
- Internal Region
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适用
- 小鼠
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This SIRT3 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)
- 原理
- Sirtuin 3 (mouse)
- 序列
- RASGIPASKL VEAH
- 特异性
- This antibody is expected to recognize both reported isoforms (NP_071878.2, NP_001171275.1). Reported variants represent identical proteins: NP_071878.2, NP_001120823.1
- 交叉反应
- 人, 小鼠, Pig, 大鼠
- 纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- 质量等级
- Verified
- 免疫原
- Peptide with sequence C-RASGIPASKLVEAH, from the internal region of the protein sequence according to NP_071878.2, NP_001171275.1.
- 亚型
- IgG
- Top Product
- Discover our top product SIRT3 Primary Antibody
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- 应用备注
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Western Blot: Approx 30 kDa band observed in Mouse Heart lysates (calculated MW of 28.8 kDa according to NP_071878.2). An additional band of unknown identity was also consistently observed at 15 kDa. This band was successfully blocked by incubation with the
Peptide ELISA: antibody detection limit dilution 1:64000.
- 说明
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Immunofluorescence: Strong expression of the protein seen in the cytoplasm of NIH3T3 cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of NIH3T3 cells. Recommended concentration: 10ug/
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Minimize freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- 抗原
- SIRT3 (Sirtuin 3 (SIRT3))
- 别名
- Sirt3 (SIRT3 产品)
- 别名
- zgc:158774 antibody, sirtuin antibody, SIR2L3 antibody, 2310003L23Rik antibody, AI848213 antibody, Sir2l3 antibody, sirtuin 3 antibody, sirt3 antibody, SIRT3 antibody, Sirt3 antibody
- 背景
- Sirt3, sirtuin 3, sirtuin 3 (silent mating type information regulation 2, homolog) 3, 2310003L23Rik, AI848213, Sir2l3, NAD-dependent deacetylase sirtuin-3, OTTMUSP00000027441, OTTMUSP00000027442, OTTMUSP00000027444, OTTMUSP00000027445, SIR2-like protein 3
- 分子量
- 28.8kDa according to NP_071878.2
- 基因ID
- 23410, 64384, 293615
- NCBI登录号
- NP_071878, NP_001171275
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