DNAJB9 抗体 (AA 61-75)
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- 抗原 See all DNAJB9 抗体
- DNAJB9 (Microvascular Endothelial Differentiation Gene 1 Protein (DNAJB9))
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抗原表位
- AA 61-75
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适用
- 人, 小鼠
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This DNAJB9 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)
- 原理
- DNAJB9 (aa61-75)
- 序列
- PDAEAKFREI AEAYE
- 交叉反应
- Cow, 犬, 人, 小鼠, Pig, 大鼠
- 纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- 质量等级
- Verified
- 免疫原
- Peptide with sequence C-PDAEAKFREIAEAYE, from the internal regoin of the protein sequence according to NP_036460.1.
- 亚型
- IgG
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- 应用备注
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Western Blot: Approx 26-27 kDa band observed in Human Liver and Kidney lysates and approx.28 kDa in Mouse Liver lysates and in preliminary testing of Rat Liver lysate (calculated MW of 25.5 kDa according to Human NP_036460.1 and 25.6 kDa according to Mouse
Peptide ELISA: antibody detection limit dilution 1:16000.
- 说明
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Immunofluorescence: Strong expression of the protein seen in the endoplasmic reticulum and cytoplasm of A431 and U2OS cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of A431 cells. R
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Minimize freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- 抗原
- DNAJB9 (Microvascular Endothelial Differentiation Gene 1 Protein (DNAJB9))
- 别名
- DNAJB9 (DNAJB9 产品)
- 背景
- DNAJB9, DnaJ (Hsp40) homolog, subfamily B, member 9, DKFZp564F1862, ERdj4, MDG1, MST049, MSTP049, dnaJ homolog subfamily B member 9, endoplasmic reticulum DnaJ homolog 4, mdg-1, microvascular endothelial differentiation gene 1 protein
- 分子量
- 25.5kDa according to NP_036460.1
- 基因ID
- 4189
- NCBI登录号
- NP_036460
- 途径
- ER-Nucleus Signaling
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