POLA1 抗体 (AA 1231-1350)
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- 抗原 See all POLA1 抗体
- POLA1 (DNA Polymerase alpha (POLA1))
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抗原表位
- AA 1231-1350
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This POLA1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 预测反应
- Human,Mouse,Rat,Dog,Horse,Rabbit
- 纯化方法
- Purified by Protein A.
- 免疫原
- KLH conjugated synthetic peptide derived from human DPOLA/DNA polymerase alpha
- 亚型
- IgG
- Top Product
- Discover our top product POLA1 Primary Antibody
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anti-DNA Polymerase alpha (POLA1) (AA 1363-1462) antibody
POLA1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3C11 unconjugated
anti-DNA Polymerase alpha (POLA1) (AA 1406-1439) antibodyPOLA1 适用: 小鼠 WB, FACS 宿主: 兔 Polyclonal RB57637 unconjugated
anti-DNA Polymerase alpha (POLA1) (N-Term) antibodyPOLA1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-DNA Polymerase alpha (POLA1) (N-Term) antibodyPOLA1 适用: 人 WB, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-DNA Polymerase alpha (POLA1) (AA 1240-1462) antibodyPOLA1 适用: 人 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-DNA Polymerase alpha (POLA1) (N-Term) antibodyPOLA1 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-DNA Polymerase alpha (POLA1) (AA 310-832) antibodyPOLA1 适用: Thermus aquaticus WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-DNA Polymerase alpha (POLA1) (AA 1-33) antibodyPOLA1 适用: 人 WB 宿主: 兔 Polyclonal RB57636 unconjugated
anti-DNA Polymerase alpha (POLA1) (AA 1363-1462) antibodyPOLA1 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-DNA Polymerase alpha (POLA1) (AA 1209-1465) antibodyPOLA1 适用: 小鼠 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200 - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 μg/μL
- 缓冲液
- 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
- 储存液
- ProClin
- 注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- 有效期
- 12 months
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- 抗原
- POLA1 (DNA Polymerase alpha (POLA1))
- 别名
- Dna Polymerase alpha (POLA1 产品)
- 别名
- pola antibody, AW321876 antibody, Pola antibody, POLA antibody, p180 antibody, polymerase (DNA directed), alpha 1, catalytic subunit S homeolog antibody, polymerase (DNA directed), alpha 1 antibody, DNA polymerase alpha 1, catalytic subunit antibody, pola1.S antibody, Pola1 antibody, POLA1 antibody
- 背景
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Synonyms: DNA polymerase alpha 1 catalytic subunit, DNA polymerase alpha catalytic subunit, DNA polymerase alpha catalytic subunit p180, DNA polymerase alpha p180 subunit, DPOLA_HUMAN, P180, p180 subunit, POLA, POLA1, Polymerase DNA directed, alpha 1, catalytic subunit, Polymerase DNA-directed, alpha 70kD.
Background: Plays an essential role in the initiation of DNA replication. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1/p180, a regulatory subunit POLA2/p70 and two primase subunits PRIM1/p49 and PRIM2/p58) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1. The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands. These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively. The reason this transfer occurs is because the polymerase alpha has limited processivity and lacks intrinsic 3' exonuclease activity for proofreading error, and therefore is not well suited for replicating long complexes.
- 基因ID
- 5422
- 途径
- SARS-CoV-2 Protein Interactome
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