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CD200 抗体 (AA 41-140)

Name: CD200 抗体 (AA 41-140)
SKU: ABIN761396
Availability: OutOfStock
Rating: 5 (1 reviews)

CD200 适用: 人 WB, ELISA, IHC (fro), IF (cc), IF (p), IHC (p) 宿主: 兔 Polyclonal unconjugated

Immunohistochemistry validation image for anti-CD200 (CD200) (AA 41-140) antibody (ABIN761396) (CD200 抗体 (AA 41-140))

IHC

Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-CD200/MOX1 Polyclonal Antibody, Unconjugated (ABIN761396) at 1:200 followed by conjugation to the secondary antibody and DAB staining (CD200 抗体 (AA 41-140))

IHC

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-CD200/MOX1 Polyclonal Antibody, Unconjugated (ABIN761396) at 1:200 followed by conjugation to the secondary antibody and DAB staining (CD200 抗体 (AA 41-140))

IHC

3 images

(1 reference)

(1 validation)

产品编号 ABIN761396

发货至: 中国

Datasheet as PDF

抗原 See all CD200 抗体

CD200
抗原表位
14

10

9

8

6

3

2

2

2

1

1

1

1

1
AA 41-140
适用
92

21

16

2

2

2

2

2

1

1
人
宿主
57

44

12
兔
Compatible Secondaries
克隆类型
59

52
多克隆
标记
39

16

13

8

8

4

3

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This CD200 antibody is un-conjugated
应用范围
51

51

32

25

15

13

12

8

7

5

4

3

3

2

1

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

交叉反应

人

预测反应

Mouse,Rat

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic peptide derived from human CD200

亚型

IgG

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anti-CD200 (CD200) (Internal Region) antibody
CD200 适用: 人 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-CD200 (CD200) (AA 31-232) antibody
CD200 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-CD200 (CD200) antibody (PE)
CD200 适用: 人 FACS 宿主: 小鼠 Monoclonal OX-104 PE

anti-CD200 (CD200) antibody (FITC)
CD200 适用: 人 FACS 宿主: 小鼠 Monoclonal OX-104 FITC

anti-CD200 (CD200) antibody
CD200 适用: 人 FACS, IHC (fro), IHC (p) 宿主: 小鼠 Monoclonal OX-104 unconjugated

anti-CD200 (CD200) (AA 56-257) antibody
CD200 适用: 人 ELISA 宿主: 小鼠 Monoclonal 6E8B11 unconjugated

anti-CD200 (CD200) (AA 31-232) antibody
CD200 适用: 人 FACS, ELISA 宿主: 兔 Monoclonal DM156 unconjugated

anti-CD200 (CD200) antibody (APC)
CD200 适用: 人 FACS 宿主: 小鼠 Monoclonal OX-104 APC

anti-CD200 (CD200) antibody
CD200 适用: 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated

anti-CD200 (CD200) (N-Term) antibody
CD200 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated

应用备注

WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

限制

仅限研究用
生效 #101517 (Immunohistochemistry)

by

Department of Pathology and Molecular Medicine, McMaster University

No.

#101517

日期

2017.07.11

抗原

CD200

Lot Number

AG06145442

Method validated

Immunohistochemistry

Positive Control

Human 7 week normal karyotype elective termination placenta

Negative Control

Placental villus from 7w gestation karyotype-normal pregnancy, stained with KLH antibody ABIN401183

Notes

Passed. ABIN761396 specifically labels the CD200 in human placental tissue.

Validation Images

Staining with ABIN761396 at dilutions 1:200, 1:250, 1:300, and 1:400 (A, B, C, and D) using a 7 week elective termination of a pregnancy that tested negative for trisomies (Clark et al. (2017) Am. J. Reprod. Immunol). Optimal staining was obtained at 1:200 dilution. Anti-KLH control (E) at 1:200 was negative.
Full Methods
Primary Antibody

ABIN761396

Secondary Antibody

Bond Polymer Refine Detection kit (Leica, DS9800, lot 49232)

Full Protocol

Fix human placental tissue in 10% buffered formalin for 24h at RT.
Process and embed tissue in paraffin.
Cut paraffin blocks with a Leica CM2255 Microtome into 4µm sections.
Affix sections to positively charged slides and air dry ON at RT.
Deway and hydrate the slides on an automated Leica BOND Rx stainer.
Antigen retrieval on the Leica BOND Rx automated stainer using epitope retrieval buffer 2 (Leica, AR9640, lot ER20172).
Stain slides with primary
Rabbit anti-CD200 antibody (AA 45-95) antibody (antibodies-online, ABIN761396, lot 980502W),
rabbit anti-CD200 Receptor 1-Like (CD200R1L) (AA 150-200) antibody(antibodies-online, ABIN1715098, lot 9A13M60), or
rabbit anti-KLH antibody (antibodies-online, ABIN401183, lot 304770)
diluted 1:200 in Power Vision IHC Super Blocker (Leica, PV6122). The staining protocol incorporates a modified Leica standard protocol IHC-F (which omits the post-primary step) and uses the standard times outlined in the machine protocol.
Stain sections with Bond Polymer Refine Detection kit (Leica, DS9800, lot 49232) containing peroxidase block, post primary antibody, polymer as well as DAB chromogen and hematoxylin counterstain for times outlined in the standard protocol IHC-F.
Remove slides from the Leica Bond Rx and then dehydrate in ethanol and clear in xylene.
Mount slides in permount mounting medium (Fisher Scientific, SP15-500, lot 162767).
After the slide-coverslip edges are dry, scan slides using Imagescope and photograph at 400x.

Experimental Notes

Several dilutions from 1:200 to 1:400 of ABIN761396 were tested and 1:200 was found to be optimal.
The staining pattern of ABIN761396 matches our results with the rabbit anti-CD200 polyclonal antibody RB846 and observations made by others. It is also consistent with staining with the CD200R1L antibody ABIN1715098).
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
Clark, Arredondo, Dhesy-Thind: "The CD200 tolerance-signaling molecule and its receptor, CD200R1, are expressed in human placental villus trophoblast and in peri-implant decidua by 5 weeks' gestation." in: Journal of reproductive immunology, Vol. 112, pp. 20-3, (2015) (PubMed).
抗原

CD200

别名

Cd200 (CD200 产品)

别名

MOX1 antibody, MOX2 antibody, MRC antibody, OX-2 antibody, CD200 antibody, Cspmo2 antibody, MRCOX2 antibody, Mox2 antibody, DKFZp459C0716 antibody, OX2 antibody, CD200 molecule antibody, Cd200 molecule antibody, CD200 antigen antibody, CD200 antibody, Cd200 antibody

背景

Synonyms: MRC, MOX1, MOX2, OX-2, OX-2 membrane glycoprotein, CD200, My033
Background: Costimulates T-cell proliferation. May regulate myeloid cell activity in a variety of tissues.

基因ID

4345

UniProt

P41217