MAP2K5 抗体 (AA 251-350)
(2 references)
(1 validation)-
- 抗原 See all MAP2K5 抗体
- MAP2K5 (Mitogen-Activated Protein Kinase Kinase 5 (MAP2K5))
-
抗原表位
- AA 251-350
-
适用
- 人, 小鼠, 大鼠
-
宿主
- 兔
-
克隆类型
- 多克隆
-
标记
- This MAP2K5 antibody is un-conjugated
-
应用范围
- Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 交叉反应
- 人, 小鼠, 大鼠
- 预测反应
- Dog,Cow,Pig,Horse,Chicken,Rabbit
- 纯化方法
- Purified by Protein A.
- 免疫原
- KLH conjugated synthetic peptide derived from human MEK5
- 亚型
- IgG
-
anti-Mitogen-Activated Protein Kinase Kinase 5 (MAP2K5) (AA 1-448) antibody
MAP2K5 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal M1-E6 unconjugated
anti-Mitogen-Activated Protein Kinase Kinase 5 (MAP2K5) (AA 1-120) antibodyMAP2K5 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 1B4 unconjugated
anti-Mitogen-Activated Protein Kinase Kinase 5 (MAP2K5) (AA 1-190) antibodyMAP2K5 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase Kinase 5 (MAP2K5) (AA 1-120) antibodyMAP2K5 适用: 人 WB, ELISA, IF, PLA 宿主: 小鼠 Monoclonal 1E5 unconjugated
anti-Mitogen-Activated Protein Kinase Kinase 5 (MAP2K5) (AA 1-190) antibodyMAP2K5 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase Kinase 5 (MAP2K5) (AA 1-190) antibodyMAP2K5 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase Kinase 5 (MAP2K5) (AA 385-412), (C-Term) antibodyMAP2K5 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase Kinase 5 (MAP2K5) (pSer137) antibodyMAP2K5 适用: 人, 大鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro), FACS 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase Kinase 5 (MAP2K5) (AA 311-315) antibodyMAP2K5 适用: 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase Kinase 5 (MAP2K5) (pSer311), (pThr315) antibodyMAP2K5 适用: 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
-
- 应用备注
-
WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200 - 限制
- 仅限研究用
-
- by
- Immunohistochemistry Core, NYU Langone
- No.
- #029645
- 日期
- 2014.03.15
- 抗原
- Lot Number
- 120828
- Method validated
- Immunohistochemistry
- Positive Control
- Human breast myoepithelial and glandular tissue
- Negative Control
- Human breast adipose tissue
- Notes
- Strong signal was detected in positive control sample, with minor background seen in negative control sample.
- Primary Antibody
- Antigen: Mitogen-Activated Protein Kinase Kinase 5 (MAP2K5)
- Catalog number: ABIN754183
- Lot number: 120828
- Secondary Antibody
- Antibody: Biotinylated goat anti-rabbit/anti-mouse (Kit)
- Lot number: D07640BA
- Full Protocol
- Immunohistochemistry was performed on a Ventana NEXes automated platform; instrument manufacturer specific reagents are italicized.
- 1. Slides were preheated in convection oven at 60°C for 30 min
- 2. Deparaffinization procedure: - 3 changes of Xylene, 5 min each - 3 changes of 100% Ethanol, 3 min each - 3 changes of 95% Ethanol, 3 min each - Rinsed in distilled water, 3 changes
- 3. Heat retrieval procedure - Slides retrieved in 10.0 mM Citrate, pH6.0 in a 1000W microwave oven (~100°C) for 15 min. - Slides were allowed to cool (in citrate) for 30 min. - Slides were washed x 3 in Distilled water
- 4. NEXes instrument procedure, iView DAB paraffin protocol (*abridged*): - Slide chamber warmed to 37°C
- 5. Slides rinsed with *reaction buffer* x3
- 6. *iView Inhibitor (H2O2)* applied and incubated for 4 min
- 7. Slides rinsed with *reaction buffer*
- 8. Antibody Application - Primary antibody diluted 1:250 in PBS (100 microliter applied/slide) - Ventana Isotype control applied neat - Slides Incubated overnight at room temperature (~12 hours ~25°C)
- 9. Slides rinsed with *reaction buffer* x3
- 10. *iView Biotinylated IgG* applied and incubated for 8 min
- 11. Slides rinsed with *reaction buffer*
- 14. *iView Streptavidin-Horseradish Peroxidase* applied and incubated for 8 min
- 15. Slides rinsed with *reaction buffer*
- 16. *iView DAB/H2O2* applied and incubated for 8 min
- 17. Slides rinsed with *reaction buffer*
- 18. *iView Copper* applied and incubated for 4 min
- 19. Slides rinsed with *reaction buffer*
- 20. Slides washed in Dawn Detergent/tap water
- 21. Counterstain Procedure - Hematoxylin (Leica 560 MX) 30 sec - Slides washed in tap water, 1 min - Decolorized (10% Acetic Acid in 70% ethanol), 1 min - Slides washed in tap water, 1 min - Bluing (Austin Clear Ammonia), 1 min - Slides washed in tap water, 1 min
- 22. Dehydration/coverslipping procedure: - 3 changes of 95% Ethanol, 3 min each - 3 changes of 100% Ethanol, 3 min each - 3 changes of Xylene, 5 min each - Mounted with Permount
- 23. Imaging: Leica SCN 400F Whole Slide Scanner with Digital Image Hub and Leica Slidepath software
- Experimental Notes
- Deviations from protocol/procedure supplied by manufacturer (attached).
- Step 1: Heated tissue 60°C for 30 minutes; manufacturer heats for 45 minutes.
- Step 2: No ethanol wash was performed during deparaffinization; manufacturer includes 1 wash of 80% ethanol for 3 minutes.
- Step 3.1: Slides were heated for 15 minutes; manufacturer provides a range of 15-20 minutes.
- Step 3.2: Slides were cooled for 30 minutes; manufacturer cools for 20 minutes.
- Step 4: Italicized reagents and incubation time are fixed instrument parameters.
- Step 5: Secondary species-specific serum block not used; manufacturer blocks with 5% normal goat serum for 2 hours.
- Step 8.1: Antibody diluted in PBS at 1:250; manufacture did not recommend diluent or dilution.
- Step 8.2.1: Primary antibody incubated at room temperature overnight; manufacturer incubates overnight 4°C with agitation.
生效 #029645 (Immunohistochemistry)!['独立验证'标志]( "成功验证")
!['独立验证'标志]( "成功验证")
Validation Images![Figure 1: Human breast tissue stained with anti-MAP2K5 (brown) and counterstained with hematoxylin.]()
Figure 1: Human breast tissue stained with anti-MAP2K5 (brown) and counterstained with hematoxylin.
![Figure 2: Human breast adipose tissue stained with anti-MAP2K5 (brown) and counterstained with hematoxylin.]()
Figure 2: Human breast adipose tissue stained with anti-MAP2K5 (brown) and counterstained with hematoxylin.
![Figure 3: Human breast tissue stained with isotype control antibody (brown) and counterstained with hematoxylin.]()
Figure 3: Human breast tissue stained with isotype control antibody (brown) and counterstained with hematoxylin.
![Figure 4: Human breast tissue stained with secondary antibody only (brown) and counterstained with hematoxylin.]()
Figure 4: Human breast tissue stained with secondary antibody only (brown) and counterstained with hematoxylin.
Full Methods -
- 状态
- Liquid
- 浓度
- 1 μg/μL
- 缓冲液
- 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
- 储存液
- ProClin
- 注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- 有效期
- 12 months
-
-
: "MicroRNA-143 regulates adipogenesis by modulating the MAP2K5-ERK5 signaling." in: Scientific reports, Vol. 4, pp. 3819, (2014) (PubMed).
: "Fatty acid synthase mediates the epithelial-mesenchymal transition of breast cancer cells." in: International journal of biological sciences, Vol. 10, Issue 2, pp. 171-80, (2014) (PubMed).
-
: "MicroRNA-143 regulates adipogenesis by modulating the MAP2K5-ERK5 signaling." in: Scientific reports, Vol. 4, pp. 3819, (2014) (PubMed).
-
- 抗原
- MAP2K5 (Mitogen-Activated Protein Kinase Kinase 5 (MAP2K5))
- 别名
- MEK5 (MAP2K5 产品)
- 别名
- Mek5 antibody, HsT17454 antibody, MAPKK5 antibody, MEK5 antibody, PRKMK5 antibody, AI324775 antibody, AI428457 antibody, Mapkk5 antibody, Prkmk5 antibody, fb73f02 antibody, wu:fb73f02 antibody, zgc:172137 antibody, ARABIDOPSIS THALIANA MITOGEN-ACTIVATED PROTEIN KINASE KINASE 5 antibody, ATMAP2K_ALPHA antibody, ATMEK5 antibody, ATMKK5 antibody, MAP KINASE KINASE 5 antibody, MAP kinase kinase 5 antibody, MAP2K_A antibody, mitogen activated protein kinase kinase 5 antibody, mitogen-activated protein kinase kinase 5 antibody, mitogen-activated protein kinase kinase 5 L homeolog antibody, MAP kinase kinase 5 antibody, Map2k5 antibody, MAP2K5 antibody, map2k5 antibody, map2k5.L antibody, MKK5 antibody
- 背景
-
Synonyms: MEK5, MAPKK5, Dual specificity mitogen-activated protein kinase kinase 5, MAPK/ERK kinase 5, MEK 5, MAP2K5, MAP kinase kinase 5
Background: Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Mediates signaling for determination of cell fate such as differentiation and survival. Plays a crucial role in the apoptosis signal transduction pathway through mitochondria-dependent caspase activation. MAP3K5/ASK1 is required for the innate immune response, which is essential for host defense against a wide range of pathogens. Mediates signal transduction of various stressors like oxidative stress as well as by receptor-mediated inflammatory signals, such as the tumor necrosis factor (TNF) or lipopolysaccharide (LPS). Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade and the p38 MAPK signal transduction cascade through the phosphorylation and activation of several MAP kinase kinases like MAP2K4/SEK1, MAP2K3/MKK3, MAP2K6/MKK6 and MAP2K7/MKK7. These MAP2Ks in turn activate p38 MAPKs and c-jun N-terminal kinases (JNKs). Both p38 MAPK and JNKs control the transcription factors activator protein-1 (AP-1).
- 基因ID
- 5607
- UniProt
- Q13163
- 途径
- MAPK Pathway, Neurotrophin Signaling Pathway
-
