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REST 抗体 (AA 401-450)

Name: REST 抗体 (AA 401-450)
SKU: ABIN747683
Availability: OutOfStock

REST 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

Raji lysates probed with NRSF Polyclonal Antibody, Unconjugated at 1:300 dilution and 4˚C overnight incubation. (REST 抗体 (AA 401-450))

Co-immunostaining for anti-Rest (red) and Six2 (green) showing higher expression levels of Rest in Six2+ cap mesenchyme (CM) surrounding ureteric bud (UB). (REST 抗体 (AA 401-450))

ISt

Formalin-fixed and paraffin embedded human gastric tissue labeled with Anti NRSF/REST Polyclonal Antibody,Unconjugated (ABIN747683) at 1:200 followed by conjugation to the secondary antibody and DAB staining. (REST 抗体 (AA 401-450))

SDS

Formalin-fixed and frozen mouse brain labeled with Anti-NRSF REST Polyclonal Antibody, unconjugated (ABIN747683) at 1:200 followed by incubation with conjugated secondary antibody (REST 抗体 (AA 401-450))

IHC

Formalin-fixed and paraffin embedded mouse brain labeled with Anti-NRSF/REST Polyclonal Antibody, Unconjugated (ABIN747683) at 1:200 followed by conjugation to the secondary antibody and DAB staining. (REST 抗体 (AA 401-450))

IHC

Formalin-fixed and paraffin embedded human gastric tissue labeled with Anti NRSF/REST Polyclonal Antibody,Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining. (REST 抗体 (AA 401-450))

IHC (p)

6 images

(3 references)

产品编号 ABIN747683

发货至: 中国

Datasheet as PDF

抗原 See all REST 抗体

REST (RE1-Silencing Transcription Factor (REST))
抗原表位
8

5

5

4

4

4

2

2

2

1

1

1

1

1

1

1
AA 401-450
适用
44

9

7

3

3

3

3

3

2

1

1

1
人, 大鼠, 小鼠
宿主
43

1
兔
Compatible Secondaries
克隆类型
43

1
多克隆
标记
23

5

3

3

2

2

1

1

1

1

1

1
This REST antibody is un-conjugated
应用范围
30

20

10

7

4

4

1

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

特异性

KO-Validated

交叉反应

人, 小鼠, 大鼠

预测反应

Cow,Pig,Horse,Chicken,Rabbit

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic peptide derived from human NRSF

亚型

IgG
anti-RE1-Silencing Transcription Factor (REST) (Middle Region) antibody
REST 适用: 人, 大鼠, 小鼠, Cow, 犬, 豚鼠, 马, 兔 WB 宿主: 兔 Polyclonal unconjugated

anti-RE1-Silencing Transcription Factor (REST) antibody
REST 适用: 人 WB, IP 宿主: 兔 Monoclonal unconjugated

anti-RE1-Silencing Transcription Factor (REST) (AA 760-1060) antibody
REST 适用: 人 WB 宿主: 兔 Polyclonal unconjugated

anti-RE1-Silencing Transcription Factor (REST) (AA 250-350) antibody
REST 适用: 人 ELISA, IF 宿主: 兔 Polyclonal unconjugated

anti-RE1-Silencing Transcription Factor (REST) (AA 20-69) antibody
REST 适用: 人, 大鼠, 小鼠, Cow, 犬, 豚鼠, 马, 兔, 猴, Bat, Pig WB 宿主: 兔 Polyclonal unconjugated

anti-RE1-Silencing Transcription Factor (REST) (C-Term) antibody
REST 适用: 人, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-RE1-Silencing Transcription Factor (REST) (Internal Region) antibody
Verified REST 适用: 人 WB, ELISA 宿主: 山羊 Polyclonal unconjugated

anti-RE1-Silencing Transcription Factor (REST) (AA 972-1021) antibody
REST 适用: 人, 猴 WB 宿主: 兔 Polyclonal unconjugated

anti-RE1-Silencing Transcription Factor (REST) (AA 542-570) antibody
REST 适用: 人 WB 宿主: 兔 Polyclonal RB36730 unconjugated

anti-RE1-Silencing Transcription Factor (REST) (AA 542-570) antibody
REST 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated

应用备注

WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

限制

仅限研究用
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
Li, Cheng, Xu, Zhang, Tokat, Dolios, Ramakrishnan, Shen, Wang, Xu: "The transcriptional coactivator Eya1 exerts transcriptional repressive activity by interacting with REST corepressors and REST-binding sequences to maintain nephron progenitor identity." in: Nucleic acids research, Vol. 50, Issue 18, pp. 10343-10359, (2022) (PubMed).

Zhang, Wang, Lu, Wang, Yuan, Yan, Cai, Chang, Zheng, Wu, Zhou: "REST regulates the cell cycle for cardiac development and regeneration." in: Nature communications, Vol. 8, Issue 1, pp. 1979, (2018) (PubMed).

Zhang, Wu, Wang, Wang, Lu, Nechiporuk, Floss, Greally, Zheng, Zhou: "Non-CpG methylation by DNMT3B facilitates REST binding and gene silencing in developing mouse hearts." in: Nucleic acids research, Vol. 45, Issue 6, pp. 3102-3115, (2017) (PubMed).
抗原

REST (RE1-Silencing Transcription Factor (REST))

别名

NRSF/REST (REST 产品)

别名

NRSF antibody, XBR antibody, 2610008J04Rik antibody, AA407358 antibody, xbr antibody, nrsf antibody, rest antibody, resta antibody, REST antibody, RE1 silencing transcription factor antibody, RE1-silencing transcription factor antibody, RE1-silencing transcription factor S homeolog antibody, REST antibody, Rest antibody, rest.S antibody, rest antibody, LOC615792 antibody

背景

Synonyms: XBR, NRSF, RE1-silencing transcription factor, Neural-restrictive silencer factor, X2 box repressor, REST
Background: Transcriptional repressor which binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Restricts the expression of neuronal genes by associating with two distinct corepressors, mSin3 and CoREST, which in turn recruit histone deacetylase to the promoters of REST-regulated genes. Mediates repression by recruiting the BHC complex at RE1/NRSE sites which acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier. Transcriptional repression by REST-CDYL via the recruitment of histone methyltransferase EHMT2 may be important in transformation suppression.

基因ID

5978

UniProt

Q13127

途径

Negative Regulation of Hormone Secretion, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Chromatin Binding, Positive Regulation of Endopeptidase Activity