EGFRviii 抗体 (AA 1-50)
(9 references)-
- 抗原 See all EGFRviii (EGFRv3) products
- EGFRviii (EGFRv3) (EGFR vIII (EGFRv3))
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抗原表位
- AA 1-50
- 适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This EGFRviii antibody is un-conjugated
- 应用范围
- Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 交叉反应
- 小鸡, 人, 小鼠
- 纯化方法
- Purified by Protein A.
- 免疫原
- KLH conjugated synthetic peptide derived from human EGFRvIII
- 亚型
- IgG
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- 应用备注
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WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200 - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 μg/μL
- 缓冲液
- 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- 有效期
- 12 months
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: "The impact of co-expression of wild-type EGFR and its ligands determined by immunohistochemistry for response to treatment with cetuximab in patients with metastatic colorectal cancer." in: Oncotarget, Vol. 8, Issue 5, pp. 7666-7677, (2018) (PubMed).
: "F25P preproinsulin abrogates the secretion of pro-growth factors from EGFRvIII cells and suppresses tumor growth in an EGFRvIII/wt heterogenic model." in: Cancer letters, Vol. 380, Issue 1, pp. 1-9, (2016) (PubMed).
: "EGFL7 is an intercellular EGFR signal messenger that plays an oncogenic role in glioma." in: Cancer letters, Vol. 384, pp. 9-18, (2016) (PubMed).
: "EGFRvIII/integrin β3 interaction in hypoxic and vitronectinenriching microenvironment promote GBM progression and metastasis." in: Oncotarget, Vol. 7, Issue 4, pp. 4680-94, (2016) (PubMed).
: "PET imaging of glioblastoma multiforme EGFR expression for therapeutic decision guidance." in: American journal of nuclear medicine and molecular imaging, Vol. 5, Issue 4, pp. 379-89, (2015) (PubMed).
: "Potentials and capabilities of the Extracellular Vesicle (EV) Array." in: Journal of extracellular vesicles, Vol. 4, pp. 26048, (2015) (PubMed).
: "An optical probe for noninvasive molecular imaging of orthotopic brain tumors overexpressing epidermal growth factor receptor." in: Molecular cancer therapeutics, Vol. 11, Issue 10, pp. 2202-11, (2012) (PubMed).
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: "The impact of co-expression of wild-type EGFR and its ligands determined by immunohistochemistry for response to treatment with cetuximab in patients with metastatic colorectal cancer." in: Oncotarget, Vol. 8, Issue 5, pp. 7666-7677, (2018) (PubMed).
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- 抗原
- EGFRviii (EGFRv3) (EGFR vIII (EGFRv3))
- Abstract
- EGFRv3 产品
- 背景
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Synonyms: EGFR vIII, EGFRvIII, Epidermal growth factor receptor variant type _, epidermal growth factor receptor isoform a variant, ERBB, HER1, ERBB1, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, EGFR
Background: Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin. Isoform 2 may act as an antagonist of EGF action.
- 基因ID
- 1956
- UniProt
- Q59FL8
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