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PKC beta 抗体 (pThr500)

Name: PKC beta 抗体 (pThr500)
SKU: ABIN741240
Availability: OutOfStock

PRKCB 适用: 人, 大鼠 WB, ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti- phospho-PRKC Polyclonal Antibody, Unconjugated (ABIN741240) at 1:200 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, PE conjugated used at 1:200 dilution for 40 minutes at 37°C and DAPI (PKC beta 抗体 (pThr500))

Molt-4 cells were fixed with 4% PFA for 10min at room temperature, permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. (PKC beta 抗体 (pThr500))

Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-phospho-PRKCZ(Thr500) Polyclonal Antibody, Unconjugated (ABIN741240) at 1:200 followed by conjugation to the secondary antibody and DAB staining (PKC beta 抗体 (pThr500))

IHC

3 images

产品编号 ABIN741240

发货至: 中国

Datasheet as PDF

抗原 See all PKC beta (PRKCB) 抗体

PKC beta (PRKCB) (Protein Kinase C, beta (PRKCB))
抗原表位
16

15

14

13

11

8

8

7

7

7

6

5

3

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1
pThr500
适用
114

92

89

14

7

6

5

2

2

2

1

1

1

1

1

1
人, 大鼠
宿主
133

4

3

2

1
兔
Compatible Secondaries
克隆类型
132

11
多克隆
标记
57

14

9

7

6

4

4

4

4

4

4

4

2

2

2

2

2

2

2

2

2

2

2
This PKC beta antibody is un-conjugated
应用范围
111

61

46

28

26

25

14

11

6

5

4

2

2

1

1

1
Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

交叉反应

人, 大鼠

预测反应

Mouse,Cow,Horse,Chicken,Rabbit

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic peptide derived from human PRKCB around the phosphorylation site of Thr500

亚型

IgG

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anti-Protein Kinase C, beta (PRKCB) (AA 601-673) antibody
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anti-Protein Kinase C, beta (PRKCB) (AA 261-341) antibody
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anti-Protein Kinase C, beta (PRKCB) (AA 1-671) antibody
PRKCB 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated

anti-Protein Kinase C, beta (PRKCB) (C-Term) antibody
PRKCB 适用: 人, 大鼠, 小鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

应用备注

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

限制

仅限研究用
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
抗原

PKC beta (PRKCB) (Protein Kinase C, beta (PRKCB))

别名

PRKCB (PRKCB 产品)

别名

PKC-beta antibody, PKCB antibody, PRKCB1 antibody, PRKCB2 antibody, A130082F03Rik antibody, PKC-Beta antibody, Pkcb antibody, Prkcb1 antibody, Prkcb2 antibody, PKC-B antibody, Prkcb antibody, prkcb1 antibody, zgc:63591 antibody, PKC antibody, PRKCB_tv2 antibody, Pkc53E antibody, prkcb1l antibody, zgc:64063 antibody, protein kinase C beta antibody, protein kinase C, beta antibody, protein kinase C, beta b antibody, protein kinase C, beta a antibody, PRKCB antibody, Prkcb antibody, prkcbb antibody, prkcba antibody

背景

Synonyms: PKCB, PRKCB1, PRKCB2, PKC-beta, Protein kinase C beta type, PKC-B, PRKCB
Background: Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This protein kinase has been reported to be involved in many different cellular functions, such as B cell activation, apoptosis induction, endothelial cell proliferation, and intestinal sugar absorption. Studies in mice also suggest that this kinase may also regulate neuronal functions and correlate fear-induced conflict behavior after stress. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

基因ID

5579

UniProt

P05771

途径

WNT signaling, TCR Signaling, Thyroid Hormone Synthesis, Nuclear Hormone Receptor Binding, Chromatin Binding, Myometrial Relaxation and Contraction, VEGF Signaling, Unfolded Protein Response, BCR Signaling