CD137 抗体 (AA 101-200)
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- 抗原 See all CD137 (TNFRSF9) 抗体
- CD137 (TNFRSF9) (Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9))
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抗原表位
- AA 101-200
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适用
- 人, 小鼠, 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This CD137 antibody is un-conjugated
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应用范围
- ELISA, Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 交叉反应
- 人, 小鼠, 大鼠
- 纯化方法
- Purified by Protein A.
- 免疫原
- KLH conjugated synthetic peptide derived from human CD137
- 亚型
- IgG
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- 应用备注
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WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400 - 限制
- 仅限研究用
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- by
- Pharmacology Department, Faculty of Medicine, Granada University
- No.
- #101793
- 日期
- 2017.09.13
- 抗原
- CD137
- Lot Number
- AG05152084
- Method validated
- Flow Cytometry
- Positive Control
- rat adipose-derived mesenchymal stem cells
- Negative Control
- cells stained with secondary antibody only
- Notes
Passed. ABIN740445 specifically recognizes Cd137 in rat adipose-derived mesenchymal stem cells.
- Primary Antibody
- ABIN740445
- Secondary Antibody
- donkey anti-rabbit Alexa Fluor 680 conjugated antibody (ThermoFisher Scientific, A10043, lot 1812322)
- Full Protocol
- Grow rat adipose-derived mesenchymal stem cells in DMEM high glucose, GLUTAMAX, pyruvate supplemented (Gibco, 31966021, lot 1774921) supplemented with 10% fetal bovine serum (Gibco, 16000044, lot 1774921) 1% penicillin/streptomycin (Gibco, 15140122, lot 1759446), at 37°C and 5% CO2 in ml on a flask to 95% confluency.
- Wash cells with PBS buffer.
- Detach cells from the flask with trypsin-versene solution (Lonza, H3BE17-161E, lot RB35937) for 3min at 37°C and 5% CO2.
- Centrifuge cells for at 400xg for 5min at RT, aspirate the supernatant.
- Resuspend cells in 1mL of 1% BSA in PBS per 2x106 cells and incubate 20min at RT.
- Incubate cells with primary rabbit anti-CD137 antibody (antibodies-online, ABIN740445, lot AG05152084) diluted 1:35 in 1% BSA in PBS buffer for 20min on ice. Include a negative control without primary antibody.
- Wash cells 1x with PBS buffer and centrifuge them for 400xg for 5min at RT.
- Resuspend cells in 175μl 1% BSA in PBS buffer.
- Incubate cells with secondary donkey anti-rabbit Alexa Fluor 680 conjugated antibody (ThermoFisher Scientific, A10043, lot 1812322) diluted 1:200 in buffer for 15min in ice.
- Wash cells 2xwith PBS buffer and centrifuge them for 400xg for 5min at RT.
- Resuspend the pellet in 500μl PBS buffer.
- Data acquisition in a BD FACSCanto II flow cytometer, equipped with two laser lines of 488nm and 633nm and two scattered light detectors (FSC and SSC). Detect fluorescence in PMTs with filters suitable for the fluorescence of the fluorophore AF680 (735LP and 780/60BP). Threshold is established in FSC. The data is saved in files in FCS format and analyzed offline with the FACSDiva software (BD).
- Experimental Notes
ABIN740445 reveals a specific fluorescence signal in rat adipose-derived mesenchymal stem cells. This is the first time that flow cytometry is performed in this type of cells using this antibody. No signal was registered in the negative control cells.
生效 #101793 (Flow Cytometry)Validation ImagesFlow cytometric analysis of cells stained with ABIN740445 and an AF680-conjugated secondary antibody (A) or the secondary antibody only (B). The top row illustrates the gating for life cells. The second and third row show dots blots and histograms respectively of the positively stained cell populations.Full Methods -
- 状态
- Liquid
- 浓度
- 1 μg/μL
- 缓冲液
- 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
- 储存液
- ProClin
- 注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- 有效期
- 12 months
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Decreased beige adipocyte number and mitochondrial respiration coincide with increased histone methyl transferase (G9a) and reduced FGF21 gene expression in Sprague-Dawley rats fed prenatal low ..." in: The Journal of nutritional biochemistry, Vol. 31, pp. 113-21, (2016) (PubMed).
: "Depletion of white adipocyte progenitors induces beige adipocyte differentiation and suppresses obesity development." in: Cell death and differentiation, (2014) (PubMed).
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Decreased beige adipocyte number and mitochondrial respiration coincide with increased histone methyl transferase (G9a) and reduced FGF21 gene expression in Sprague-Dawley rats fed prenatal low ..." in: The Journal of nutritional biochemistry, Vol. 31, pp. 113-21, (2016) (PubMed).
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- 抗原
- CD137 (TNFRSF9) (Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9))
- 别名
- CD137 (TNFRSF9 产品)
- 别名
- TNFRSF9 antibody, zgc:136557 antibody, 4-1BB antibody, CD137 antibody, CDw137 antibody, ILA antibody, A930040I11Rik antibody, AA408498 antibody, AI325004 antibody, Cd137 antibody, Ly63 antibody, tumor necrosis factor receptor superfamily member 9 antibody, TNF receptor superfamily member 9 antibody, tumor necrosis factor receptor superfamily, member 9a antibody, tumor necrosis factor receptor superfamily, member 9 antibody, TNFRSF9 antibody, tnfrsf9a antibody, Tnfrsf9 antibody
- 背景
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Synonyms: ILA, 4-1BB, CD137, CDw137, Tumor necrosis factor receptor superfamily member 9, 4-1BB ligand receptor, T-cell antigen 4-1BB homolog, T-cell antigen ILA, TNFRSF9
Background: Receptor for TNFSF9/4-1BBL. Possibly active during T cell activation.
- 基因ID
- 3604
- UniProt
- Q07011
- 途径
- Cancer Immune Checkpoints
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