Protein A 抗体
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- 抗原 See all Protein A 抗体
- Protein A
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适用
- Staphylococcus aureus
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Protein A antibody is un-conjugated
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应用范围
- ELISA, Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 交叉反应
- Bacteria
- 交叉反应 （详细）
- S. aureus
- 纯化方法
- Purified by Protein A.
- 免疫原
- Recombinant protein A
- 亚型
- IgG
- Top Product
- Discover our top product Protein A Primary Antibody
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anti-Protein A antibody (HRP)
适用: Staphylococcus aureus ELISA, WB 宿主: 小鸡 Polyclonal HRP
anti-Protein A antibody适用: Staphylococcus aureus IP, Con 宿主: 山羊 Polyclonal unconjugated
anti-Protein A antibody适用: Staphylococcus aureus ELISA, WB, IP, DB 宿主: 山羊 Polyclonal unconjugated
anti-Protein A antibody (Rhodamine)适用: Staphylococcus aureus IF 宿主: 山羊 Polyclonal Rhodamine
anti-Protein A antibody (Texas Red (TR))适用: Staphylococcus aureus FACS, FM 宿主: 山羊 Polyclonal Texas Red (TR)
anti-Protein A antibody (HRP)适用: Staphylococcus aureus ELISA, WB, IHC 宿主: 山羊 Polyclonal HRP
anti-Protein A antibody (FITC)适用: Staphylococcus aureus FACS 宿主: 山羊 Polyclonal FITC
anti-Protein A antibody (Biotin)适用: Staphylococcus aureus ELISA, WB, IHC 宿主: 山羊 Polyclonal Biotin
anti-Protein A antibody (Texas Red (TR))适用: Staphylococcus aureus FACS, IM 宿主: 山羊 Polyclonal Texas Red (TR)
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- 应用备注
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WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200 - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 μg/μL
- 缓冲液
- 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- 有效期
- 12 months
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- 抗原
- Protein A
- Abstract
- Protein A 产品
- 别名
- alpha protein antibody, repB antibody
- 背景
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Synonyms: Immunoglobulin G binding protein A, Staphylococcal protein A, SPA, gG-binding protein A, Staphylococcus aureus.
Background: Protein A is a 40-60 kDa surface protein originally found in the cell wall of the bacteria Staphylococcus aureus (SPA). SPA binds proteins from many of mammalian species, most notably IgGs, and helps inhibit phagocytic engulfment and acts as an immunological disguise via this type of interaction, thus the bacteria will disrupts opsonization and phagocytosis. SPA is known to bind with Fc region of immunoglobulins preferentially through interaction with the VH3 variable region of the heavy chain. SPA has been shown to bind with high affinity to human IgG1 and IgG2 as well as mouse IgG2a and IgG2b, whereas bind with moderate affinity to human IgM, IgA and IgE as well as mouse IgG3 and IgG1. SPA is often produced in E. coli and is practically coupled to other molecules such as enzymes, biotin, radioactive iodine for use in immunology and other biological research. SPA is also immobilized onto solid supports such as agarose beads for total IgG purifying or interest protein or protein complex identifying in immunoprecipitation studies.
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