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SKP2 抗体 (AA 351-424)

Name: SKP2 抗体 (AA 351-424)
SKU: ABIN737601
Availability: OutOfStock

SKP2 适用: 人, 小鼠, 大鼠 WB, ELISA, FACS, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated

Formalin-fixed and paraffin embedded human thyroid carcinoma labeled with Anti-SKP2/skp2 p45 Polyclonal Antibody, Unconjugated (ABIN737601) at 1:200 followed by conjugation to the secondary antibody and DAB staining. (SKP2 抗体 (AA 351-424))

IHC

MCF-7 cells were stained with SKP2 Polyclonal Antibody, Unconjugated at 1:500 in PBS and incubated for two hours at 37°C followed by Goat Anti-Rabbit IgG (H+L) Cy3 conjugated secondary antibody. (SKP2 抗体 (AA 351-424))

IF (cc)

293T lysates probed with SKP2 Polyclonal Antibody, Unconjugated at 1:500 dilution and 4˚C overnight incubation. (SKP2 抗体 (AA 351-424))

Formalin-fixed and paraffin embedded human thyroid carcinoma labeled with Anti-SKP2/skp2 p45 Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining. (SKP2 抗体 (AA 351-424))

IHC (p)

Hela cells probed with SKP2 Polyclonal Antibody, Unconjugated (green) at 1:100 for 30 minutes followed by a PE conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange). (SKP2 抗体 (AA 351-424))

FACS

5 images

(4 references)

产品编号 ABIN737601

发货至: 中国

Datasheet as PDF

抗原 See all SKP2 抗体

SKP2 (S-Phase Kinase-Associated Protein 2 (SKP2))
抗原表位
15

8

8

7

7

2

2

1

1

1

1

1

1

1

1

1

1

1
AA 351-424
适用
70

35

33

5

5

2

1

1

1

1
人, 小鼠, 大鼠
宿主
63

8

1
兔
Compatible Secondaries
克隆类型
64

7
多克隆
标记
41

4

3

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This SKP2 antibody is un-conjugated
应用范围
50

20

18

17

12

12

12

12

12

7

2

2

1

1

1
Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro))

交叉反应

人, 小鼠, 大鼠

预测反应

Cow,Rabbit

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic peptide derived from human Skp2

亚型

IgG
anti-S-Phase Kinase-Associated Protein 2 (SKP2) (C-Term) antibody
SKP2 适用: 人, 小鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-S-Phase Kinase-Associated Protein 2 (SKP2) (AA 1-100) antibody
SKP2 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated

anti-S-Phase Kinase-Associated Protein 2 (SKP2) antibody
SKP2 适用: 人 WB, IHC 宿主: 兔 Monoclonal unconjugated

anti-S-Phase Kinase-Associated Protein 2 (SKP2) (AA 43-397) antibody
SKP2 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-S-Phase Kinase-Associated Protein 2 (SKP2) (AA 1-424) antibody
SKP2 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-S-Phase Kinase-Associated Protein 2 (SKP2) antibody
SKP2 适用: 人 ELISA, IHC, IF 宿主: 兔 Monoclonal 6D4 unconjugated Recombinant Antibody

anti-S-Phase Kinase-Associated Protein 2 (SKP2) antibody
SKP2 适用: 人 ELISA, IF, FACS 宿主: 兔 Monoclonal 3C11 unconjugated Recombinant Antibody

anti-S-Phase Kinase-Associated Protein 2 (SKP2) (AA 1-424) antibody
SKP2 适用: 人 WB, PLA 宿主: 小鼠 Polyclonal unconjugated

anti-S-Phase Kinase-Associated Protein 2 (SKP2) (N-Term) antibody
SKP2 适用: 人, 小鼠, 大鼠 WB, IHC, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated

anti-S-Phase Kinase-Associated Protein 2 (SKP2) antibody
SKP2 适用: 人 WB, ELISA, IHC, IP, IHC (p) 宿主: 小鼠 Monoclonal unconjugated

应用备注

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

限制

仅限研究用
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
Pan, Li, Zhong, Chen, Chen, Gao, Wu, Guo: "RIG-I inhibits pancreatic β cell proliferation through competitive binding of activated Src." in: Scientific reports, Vol. 6, pp. 28914, (2016) (PubMed).

Yang, Wang, Wang, Zhang, Zhang, Lu, Wang: "mTOR is involved in 17?-estradiol-induced, cultured immature boar Sertoli cell proliferation via regulating the expression of SKP2, CCND1, and CCNE1." in: Molecular reproduction and development, Vol. 82, Issue 4, pp. 305-14, (2015) (PubMed).

Jiao, Yi, Rong, Kee, Zhong: "MicroRNA-1285 regulates 17?-estradiol-inhibited immature boar Sertoli cell proliferation via adenosine monophosphate-activated protein kinase activation." in: Endocrinology, pp. en20141982, (2015) (PubMed).

Sun, Yang, Luo, Wang, Chen, Zhang, Wang, Li: "Thyroid hormone inhibits the proliferation of piglet Sertoli cell via PI3K signaling pathway." in: Theriogenology, (2014) (PubMed).
抗原

SKP2 (S-Phase Kinase-Associated Protein 2 (SKP2))

别名

SKP2 (SKP2 产品)

别名

FBXL1 antibody, FBL1 antibody, FLB1 antibody, p45 antibody, RGD1562456 antibody, S-phase kinase-associated protein 2 (p45) antibody, S-phase kinase associated protein 2 antibody, Skp2 antibody, SKP2 antibody

背景

Synonyms: p45, FBL1, FLB1, FBXL1, S-phase kinase-associated protein 2, Cyclin-A/CDK2-associated protein p45, F-box protein Skp2, F-box/LRR-repeat protein 1, p45skp2, SKP2
Background: Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. Specifically recognizes phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. Degradation of CDKN1B/p27kip also requires CKS1. Recognizes target proteins ORC1, CDT1, RBL2, KMT2A/MLL1, CDK9, RAG2, FOXO1, UBP43, and probably MYC, TOB1 and TAL1. Degradation of TAL1 also requires STUB1. Recognizes CDKN1A in association with CCNE1 or CCNE2 and CDK2. Promotes ubiquitination and destruction of CDH1 in a CK1-Dependent Manner, thereby regulating cell migration.

基因ID

6502

UniProt

Q13309

途径

Mitotic G1-G1/S Phases