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PD-1 抗体 (AA 201-288)

Name: PD-1 抗体 (AA 201-288)
SKU: ABIN735608
Availability: OutOfStock
Rating: 5 (1 reviews)

PDCD1 适用: 人, 小鼠, 大鼠 FACS, WB, ELISA, IHC (p), IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated

Immunohistochemistry validation image for anti-Programmed Cell Death 1 (PDCD1) (AA 201-288) antibody (ABIN735608) (PD-1 抗体 (AA 201-288))

IHC

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-D-1/CD279 Polyclonal Antibody, Unconjugated (ABIN735608) at 1:200 followed by conjugation to the secondary antibody and DAB staining. (PD-1 抗体 (AA 201-288))

IHC

Formalin-fixed and paraffin embedded mouse colon labeled with Anti-PD-1/CD279 Polyclonal Antibody, Unconjugated (ABIN735608) at 1:200 followed by conjugation to the secondary antibody and DAB staining. (PD-1 抗体 (AA 201-288))

IHC

L1 human kidney lysates L2 rat brain lysates probed with Anti PD-1/CD279 Polyclonal Antibody, Unconjugated (ABIN735608) at 1:200 overnight at 4 °C. (PD-1 抗体 (AA 201-288))

SDS

5 images

(2 references)

(1 validation)

产品编号 ABIN735608

发货至: 中国

Datasheet as PDF

抗原 See all PD-1 (PDCD1) 抗体

PD-1 (PDCD1) (Programmed Cell Death 1 (PDCD1))
抗原表位
15

8

5

5

4

4

3

3

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 201-288
适用
144

76

24

5

3

1
人, 小鼠, 大鼠
宿主
81

65

25

13

11

3

1

1
兔
Compatible Secondaries
克隆类型
136

61

2
多克隆
标记
94

15

13

11

7

5

4

4

3

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This PD-1 antibody is un-conjugated
应用范围
129

59

43

40

35

18

13

12

12

9

9

8

8

8

5

4

4

4

2

2

1

1

1

1

1
Flow Cytometry (FACS), Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

交叉反应

人, 小鼠, 大鼠

预测反应

Cow,Horse,Rabbit

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic peptide derived from human PD-1

亚型

IgG
anti-Programmed Cell Death 1 (PDCD1) (AA 41-132) antibody
PDCD1 适用: 人 WB, ELISA, IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Programmed Cell Death 1 (PDCD1) (AA 192-288) antibody
PDCD1 适用: 人 FACS, WB, ELISA 宿主: 小鼠 Monoclonal 3B3D9 unconjugated

anti-Programmed Cell Death 1 (PDCD1) (AA 192-288) antibody
PDCD1 适用: 人 FACS, WB, ELISA 宿主: 小鼠 Monoclonal 8G5B12 unconjugated

anti-Programmed Cell Death 1 (PDCD1) (AA 21-170) antibody
PDCD1 适用: 人 FACS, WB, IHC, ELISA, ICC, Neut 宿主: 小鼠 Monoclonal 3E12A10 unconjugated

anti-Programmed Cell Death 1 (PDCD1) (AA 21-170) antibody
PDCD1 适用: 人 FACS, WB, IHC, ELISA, ICC, Neut 宿主: 小鼠 Monoclonal 1D6F6 unconjugated

anti-Programmed Cell Death 1 (PDCD1) (AA 21-170) antibody
PDCD1 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated

anti-Programmed Cell Death 1 (PDCD1) (AA 1-288) antibody
PDCD1 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated

anti-Programmed Cell Death 1 (PDCD1) antibody
PDCD1 适用: 人 IHC, ELISA, StM, Coat 宿主: 小鼠 Monoclonal PDCD1-2720 unconjugated

anti-Programmed Cell Death 1 (PDCD1) (AA 41-132) antibody
PDCD1 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C1 unconjugated

anti-Programmed Cell Death 1 (PDCD1) (AA 1-212) antibody
PDCD1 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated

应用备注

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

限制

仅限研究用
生效 #104535 (Immunohistochemistry)

by

TRON

No.

#104535

日期

2023.11.29

抗原

PD-1

Lot Number

BC04411027

Method validated

Immunohistochemistry

Positive Control

FFPE human tonsil

Negative Control

Notes

Passed. The PD-1 antibody (AA 201-288) ABIN735608 specifically stains cells within the germinal zone in human tonsil.

Validation Images

IHC staining of human tonsil with anti-PD-1 antibody ABIN735608 (1:250) and counterstain with hematoxylin.

IHC staining of human tonsil with anti-PD-1 antibody ABIN735608 (1:500) and counterstain with hematoxylin.
Full Methods
Primary Antibody

ABIN735608

Secondary Antibody

HRP-conjugated goat anti-rabbit antibody (medac diagnostics, DPVR-110HRP, Lot 101122)

Full Protocol

Dewax and rehydrate tissue sections on glass slides in a descending xylene and graded alcohol series:
xylene 2x for 5 min.
100 % (v/v) ethanol 2x for 5 min.
96 % (v/v) ethanol 2x for 5 min.
70 % (v/v) ethanol 2x for 5 min.
Wash slides 2x 5 min with ddH2O.
Cook slides for 30 min at 95 °C in citrate buffer pH 6.
Wash slides 2x 3 min with ddH2O.
Incubate slides in 0.3 % (v/v) H2O2 in PBS for 15 min to quench endogenous peroxidase.
Wash slides 3x 3 min in 1X PBS.
Block slides in 10 % goat serum in PBS for 30 min at RT.
Incubate slides with primary rabbit anti-PD-1 antibody (antibodies-online, ABIN735608, lot BC04411027) diluted 1:250 and 1:500 in 10 % goat serum in PBS ON at 4 °C in a humidified chamber.
Wash slides 3x 3 min in 1X PBS.
Incubate slides with secondary HRP-conjugated goat anti-rabbit antibody (medac diagnostics, DPVR-110HRP, Lot 101122) ready-to-use in 10 % goat serum in PBS for 30 min at RT in a humidified chamber.
Wash slides 3x 3 min in 1X PBS.
HRP development.
Counterstain slides with hematoxylin solution (Carl Roth, T865.2).
Wash slides for 15 min under running tap water.
Dehydrate sections in an ascending alcohol-xylene series:
70% ethanol 2x for 10 sec.
96% ethanol 2x for 10 sec.
100% ethanol 2x for 10 sec.
xylene 2x for 10 sec.
Mount sections with Entellan new mounting medium (Sigma-Aldrich, 1008690500).
Dry sections.
Image acquisition on an Aperio CS2 Digital Pathology Slide Scanner (Leica), Magnification 40x.

Experimental Notes

Staining with ABIN735608 the signal at 1:250 and 1:500 antibody dilution in human tonsil was sharp and localized in the cells within the germinal zone (as expected).
ABIN735608 reactivity was also tested in mouse samples. However, in mouse spleen, the signal was almost absent and very weak at Ab dilution 1:250 and 1:500. No signal was detected in mouse pancreas. Even increasing the Ab concentration to 1:150 dilution, in mouse tumor tissue, ABIN735608 detected a very weak, unclear, and sporadic signal. The quality of the signal does not allow the possibility of background staining to be excluded.
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
Zhang, Niyazi, Hong, Tuluwengjiang, Zhang, Zhang, Su, Bao: "Effect of EBI3 on radiation-induced immunosuppression of cervical cancer HeLa cells by regulating Treg cells through PD-1/PD-L1 pathway." in: Tumour biology, Vol. 39, Issue 3, pp. 1010428317692240, (2017) (PubMed).

Szabó, Gáspár, Dajnoki, Papp, Fábos, Szegedi, Zeher: "Expansion of circulating follicular T helper cells associates with disease severity in childhood atopic dermatitis." in: Immunology letters, Vol. 189, pp. 101-108, (2017) (PubMed).
抗原

PD-1 (PDCD1) (Programmed Cell Death 1 (PDCD1))

别名

PD-1/CD279 (PDCD1 产品)

别名

CD279 antibody, PD-1 antibody, PD1 antibody, SLEB2 antibody, hPD-1 antibody, hPD-l antibody, Ly101 antibody, Pdc1 antibody, programmed cell death 1 antibody, PDCD1 antibody, Pdcd1 antibody

背景

Synonyms: PD1, PD-1, CD279, SLEB2, hPD-1, hPD-l, hSLE1, Programmed cell death protein 1, Protein PD-1, PDCD1
Background: Inhibitory cell surface receptor involved in the regulation of T-cell function during immunity and tolerance. Upon ligand binding, inhibits T-cell effector functions in an antigen-specific manner. Possible cell death inducer, in association with other factors.

基因ID

5133

UniProt

Q15116

途径

Cancer Immune Checkpoints