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Neuroglobin 抗体 (AA 51-151)

Name: Neuroglobin 抗体 (AA 51-151)
SKU: ABIN735488
Availability: OutOfStock

NGB 适用: 大鼠 ELISA, IF (cc), IF (p), IHC (fro), IHC (p) 宿主: 兔 Polyclonal unconjugated

Formalin-fixed and paraffin embedded human brain tissue labeled with Anti-NGB/neuroglobin, Unconjugated (ABIN735488) followed by conjugation to the secondary antibody (Neuroglobin 抗体 (AA 51-151))

IHC

Paraformaldehyde-fixed, paraffin embedded rat brain, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with NGB/neuroglobin Polyclonal Antibody, Unconjugated at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining (Neuroglobin 抗体 (AA 51-151))

IHC (p)

2 images

产品编号 ABIN735488

发货至: 中国

Datasheet as PDF

抗原 See all Neuroglobin (NGB) 抗体

Neuroglobin (NGB)
抗原表位
14

9

7

6

1

1
AA 51-151
适用
34

18

3
大鼠
宿主
45

5

1
兔
Compatible Secondaries
克隆类型
47

4
多克隆
标记
17

5

5

5

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This Neuroglobin antibody is un-conjugated
应用范围
25

24

14

12

12

4

3

3

2

2

2

1

1
ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

交叉反应

大鼠

预测反应

Human,Mouse,Cow,Pig,Rabbit

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic peptide derived from mouse NGB

亚型

IgG
anti-Neuroglobin (NGB) (AA 1-151) antibody
NGB 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Neuroglobin (NGB) (AA 1-151) antibody
NGB 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2B5-A7 unconjugated

anti-Neuroglobin (NGB) (AA 1-151) antibody
NGB 适用: 人 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated

anti-Neuroglobin (NGB) antibody
NGB 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Neuroglobin (NGB) antibody
NGB 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-Neuroglobin (NGB) (AA 1-151) antibody
NGB 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated

anti-Neuroglobin (NGB) antibody
NGB 适用: 人, 大鼠, 小鼠 IF 宿主: 兔 Polyclonal unconjugated

anti-Neuroglobin (NGB) antibody
NGB 适用: 人 WB, IF, IC 宿主: 兔 Polyclonal unconjugated

anti-Neuroglobin (NGB) (AA 51-151) antibody (Biotin)
NGB 适用: 大鼠 ELISA, IHC (fro), IHC (p) 宿主: 兔 Polyclonal Biotin

anti-Neuroglobin (NGB) (AA 51-151) antibody (AbBy Fluor® 488)
NGB 适用: 大鼠 IF (cc), IF (p) 宿主: 兔 Polyclonal AbBy Fluor® 488

应用备注

ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

限制

仅限研究用
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
抗原

Neuroglobin (NGB)

别名

NGB/neuroglobin (NGB 产品)

别名

NGB antibody, ngb antibody, neuroglobin antibody, neuroglobin L homeolog antibody, NGB antibody, Ngb antibody, ngb antibody, ngb.L antibody

背景

Synonyms: Neuroglobin, Ngb
Background: Involved in oxygen transport in the brain. Hexacoordinate globin, displaying competitive binding of oxygen or the distal His residue to the iron atom. Not capable of penetrating cell membranes. The deoxygenated form exhibits nitrite reductase activity inhibiting cellular respiration via NO-binding to cytochrome c oxidase. Involved in neuroprotection during oxidative stress. May exert its anti-apoptotic activity by acting to reset the trigger level of mitochondrial cytochrome c release necessary to commit the cells to apoptosis.

基因ID

64242

UniProt

Q9ER97