Elastin 抗体 (AA 681-786)
(4 references)
(1 validation)-
- 抗原 See all Elastin (ELN) 抗体
- Elastin (ELN)
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抗原表位
- AA 681-786
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适用
- 人, 小鼠, 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Elastin antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Flow Cytometry (FACS)
- 交叉反应
- 人, 小鼠, 大鼠
- 纯化方法
- Purified by Protein A.
- 免疫原
- KLH conjugated synthetic peptide derived from human Elastin
- 亚型
- IgG
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anti-Elastin (ELN) (AA 392-645) antibody
ELN 适用: 人 WB, ELISA, ICC, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Elastin (ELN) (AA 266-443) antibodyELN 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Elastin (ELN) antibodyELN 适用: 人 IHC, StM 宿主: 兔 Monoclonal ELN-3131R unconjugated Recombinant Antibody
anti-Elastin (ELN) antibodyELN 适用: 人 IHC, ELISA, Coat, StM 宿主: 小鼠 Monoclonal ELN-1981 unconjugated
anti-Elastin (ELN) antibodyELN 适用: 人 IHC 宿主: 兔 Monoclonal MSVA-648R unconjugated Recombinant Antibody
anti-Elastin (ELN) antibodyELN 适用: 人 IHC, ELISA, IHC (p), IF, RIA 宿主: 兔 Polyclonal unconjugated
anti-Elastin (ELN) (AA 392-645) antibodyELN 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C3 unconjugated
anti-Elastin (ELN) antibodyELN 适用: 人, 小鼠, Cow, 小鸡 WB, IHC, ELISA, IP, IHC (p), IF 宿主: 小鼠 Monoclonal 10B8 unconjugated
anti-Elastin (ELN) (AA 27-200) antibodyELN 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Elastin (ELN) (AA 101-200) antibodyELN 适用: 人, 小鼠, 大鼠 WB, ELISA, ICC, IHC (p), IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200 - 限制
- 仅限研究用
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- by
- Beth Israel Deaconess Medical Center Confocal Imaging Core
- No.
- #028751
- 日期
- 2013.09.25
- 抗原
- Lot Number
- 120306
- Method validated
- Immunohistochemistry
- Positive Control
- Mouse heart tissue
- Negative Control
- Mouse adipose tissue
- Notes
- Signal was detected in positive control tissue and not detected in negative control tissue.
- Primary Antibody
- Antibody: Elastin (ELN) (C-Term) antibody
- Catalog number: ABIN734003
- Lot number: 120306
- Secondary Antibody
- Antibody: Donkey anti-Rabbit IgG Antibody (Biotinylated)
- Full Protocol
- Immunohistochemistry was performed manually.
- Sections were deparaffinized and rehydrated.
- Sections were heated to 98°C for 10 min in 10 mM citrate buffer pH 6.0 for antigen retrieval.
- Sections were incubated in 3% hydrogen peroxide for 10 min at room temperature to block endogenous peroxidase.
- Sections were washed x 3 in Tris buffered saline (TBS).
- Sections were blocked in 5% normal donkey serum for 60 min at room temperature.
- Sections were incubated with primary antibody diluted 1:1000 in 5% normal donkey serum in TBS. Incubated at 4°C overnight.
- Sections were washed x 3 in Tris buffered saline.
- Sections were incubated with secondary antibody diluted 1:400 in 5% normal donkey serum in TBS. Incubated for 60 min at room temperature.
- Sections were washed x 3 in Tris buffered saline.
- Sections were incubated with Vectastain ABC kit (Vector Lab PK6100) to enhance signal.
- Sections were incubated with DAB chromogenic substrate (Vector Lab SK-4105) for 60 seconds at room temperature.
- Sections were washed x 3 in Distilled Water.
- Sections were counterstained with hematoxylin for 1 min.
- Sections were washed x 1 in Distilled Water.
- Sections were dehydrated, mounted and photographed under a Zeiss AxioImager M1 light microscope.
- Experimental Notes
- None
生效 #028751 (Immunohistochemistry)!['独立验证'标志]( "成功验证")
!['独立验证'标志]( "成功验证")
Validation Images![Figure 1: Immunostaining of Elastin antibody. Panel 1: Staining of mouse heart tissue stained with anti-Elastin antibody (brown). Panel 2: Staining of mouse adipose tissue with anti-Elastin antibody. Panel 3: Staining of mouse heart tissue with isotype control antibody. Panel 4: Staining of mouse heart tissue with secondary antibody only. All panels are counterstained with hematoxylin (blue).]()
Figure 1: Immunostaining of Elastin antibody. Panel 1: Staining of mouse heart tissue stained with anti-Elastin antibody (brown). Panel 2: Staining of mouse adipose tissue with anti-Elastin antibody. Panel 3: Staining of mouse heart tissue with isotype control antibody. Panel 4: Staining of mouse heart tissue with secondary antibody only. All panels are counterstained with hematoxylin (blue).
Full Methods -
- 状态
- Liquid
- 浓度
- 1 μg/μL
- 缓冲液
- 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
- 储存液
- ProClin
- 注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- 有效期
- 12 months
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: "Vasoprotective effects of urocortin 1 against atherosclerosis in vitro and in vivo." in: PLoS ONE, Vol. 9, Issue 12, pp. e110866, (2014) (PubMed).
: "Stimulatory effects of cardiotrophin 1 on atherosclerosis." in: Hypertension, Vol. 62, Issue 5, pp. 942-50, (2013) (PubMed).
: "Piezoelectric PU/PVDF electrospun scaffolds for wound healing applications." in: Colloids and surfaces. B, Biointerfaces, Vol. 96, pp. 29-36, (2012) (PubMed).
: "[Syntheses of 1,3,4-thiadiazole derivatives]." in: Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan, Vol. 90, Issue 1, pp. 95-8, (1970) (PubMed).
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: "Vasoprotective effects of urocortin 1 against atherosclerosis in vitro and in vivo." in: PLoS ONE, Vol. 9, Issue 12, pp. e110866, (2014) (PubMed).
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- 抗原
- Elastin (ELN)
- 别名
- Elastin (ELN 产品)
- 别名
- SVAS antibody, WBS antibody, WS antibody, AI385707 antibody, AI480567 antibody, E030024M20Rik antibody, RATTREL11 antibody, TREL11 antibody, Trela antibody, Trela26 antibody, ELN antibody, elastin antibody, ELN antibody, Eln antibody, LOC574132 antibody, Tcur_2481 antibody, Kfla_2289 antibody, LOC100620140 antibody
- 背景
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Synonyms: WS, WBS, SVAS, Elastin, Tropoelastin, ELN
Background: Major structural protein of tissues such as aorta and nuchal ligament, which must expand rapidly and recover completely. Molecular determinant of the late arterial morphogenesis, stabilizing arterial structure by regulating proliferation and organization of vascular smooth muscle (By similarity).
- 基因ID
- 2006
- UniProt
- P15502
- 途径
- Regulation of Actin Filament Polymerization
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