我们推荐在您的浏览器上开启Javascript，以获得最佳效果。

Sonic Hedgehog 抗体 (AA 21-120)

Name: Sonic Hedgehog 抗体 (AA 21-120)
SKU: ABIN731108
Availability: OutOfStock

SHH 适用: 人, 小鼠, 大鼠, Pig WB, ELISA, IF (cc), IHC (p), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

Mouse embryo lysates probed with Rabbit Anti-Shh Polyclonal Antibody, Unconjugated at 1:5000 for 90 min at 37˚C. (Sonic Hedgehog 抗体 (AA 21-120))

Formalin-fixed and paraffin embedded human gastric cancer labeled with Anti-SHH Polyclonal Antibody, Unconjugated (ABIN731108) followed by conjugation to the secondary antibody and DAB staining (Sonic Hedgehog 抗体 (AA 21-120))

IHC

Human A549 cells probed with Rabbit Anti-Shh Polyclonal Antibody, Unconjugated . (Sonic Hedgehog 抗体 (AA 21-120))

FACS

This image was generously submitted by Dr. (Sonic Hedgehog 抗体 (AA 21-120))

IF (p)

Lane 1: mouse embryo lysates Lane 2: rat brain lysates probed with Anti ShhPolyclonal Antibody, Unconjugated (ABIN731108) at 1:200 in 4 °C. (Sonic Hedgehog 抗体 (AA 21-120))

SDS

Mouse stomach lysates probed with Shh Polyclonal Antibody, unconjugated at 1:300 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C. (Sonic Hedgehog 抗体 (AA 21-120))

6 images

(10 references)

产品编号 ABIN731108

发货至: 中国

Datasheet as PDF

抗原 See all Sonic Hedgehog (SHH) 抗体

Sonic Hedgehog (SHH)
抗原表位
14

12

9

6

4

4

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1
AA 21-120
适用
65

49

36

16

10

6

6

5

2

2

2

1

1

1

1

1

1

1
人, 小鼠, 大鼠, Pig
宿主
72

12

8

1

1
兔
Compatible Secondaries
克隆类型
67

27
多克隆
标记
51

8

4

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This Sonic Hedgehog antibody is un-conjugated
应用范围
68

27

23

13

12

10

9

7

6

6

5

3

3

1
Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

交叉反应

人, 小鼠, Pig, 大鼠

预测反应

Cow,Horse,Chicken,Rabbit

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic peptide derived from human Shh

亚型

IgG
anti-Sonic Hedgehog (SHH) (AA 26-161) antibody
SHH 适用: 人 WB, IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 5H4 unconjugated

anti-Sonic Hedgehog (SHH) (C-Term) antibody
Verified SHH 适用: 人, 小鼠 WB, ELISA, IF, FACS 宿主: 山羊 Polyclonal unconjugated

anti-Sonic Hedgehog (SHH) (N-Term) antibody
SHH 适用: 人, 小鼠, 大鼠, 斑马鱼, 犬, Cow, 豚鼠, 马, 山羊, 兔 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Sonic Hedgehog (SHH) (AA 36-193) antibody
SHH 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Sonic Hedgehog (SHH) (AA 36-193) antibody
SHH 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal D6 unconjugated

anti-Sonic Hedgehog (SHH) (AA 58-91), (N-Term) antibody
SHH 适用: 人, 小鼠, 大鼠 WB, IF, IHC (p), FACS 宿主: 兔 Polyclonal RB52387 unconjugated

anti-Sonic Hedgehog (SHH) (AA 24-197) antibody
SHH 适用: 人, 大鼠, 犬, 猴 WB, IF 宿主: 小鼠 Monoclonal 10H6 unconjugated

anti-Sonic Hedgehog (SHH) (AA 203-218), (N-Term) antibody
SHH 适用: 人, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Sonic Hedgehog (SHH) (AA 237-399) antibody
SHH 适用: 大鼠 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated

anti-Sonic Hedgehog (SHH) (AA 28-193) antibody
SHH 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

应用备注

WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

限制

仅限研究用
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
Fan, Li, Liu, Cong, Zhao, Cao, Zheng: "Doxorubicin combined with low intensity ultrasound suppresses the growth of oral squamous cell carcinoma in culture and in xenografts." in: Journal of experimental & clinical cancer research : CR, Vol. 36, Issue 1, pp. 163, (2018) (PubMed).

Yang, Wu, Feng, Huang, Liu, Liu, Chen: "Vitamin C plus hydrogel facilitates bone marrow stromal cell-mediated endometrium regeneration in rats." in: Stem cell research & therapy, Vol. 8, Issue 1, pp. 267, (2017) (PubMed).

Song, Du, Tao: "Blockade of sonic hedgehog signaling decreases viability and induces apoptosis in retinoblastoma cells: The key role of the PI3K/Akt pathway." in: Oncology letters, Vol. 14, Issue 4, pp. 4099-4105, (2017) (PubMed).

Wang, Zhang, Bai, Wang, Du: "Baicalin Attenuates Alcoholic Liver Injury through Modulation of Hepatic Oxidative Stress, Inflammation and Sonic Hedgehog Pathway in Rats." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 39, Issue 3, pp. 1129-40, (2016) (PubMed).

Bai, Lu, Lin, Xu, Hu, Liang, Hong, Chen: "Sonic hedgehog-mediated epithelial-mesenchymal transition in renal tubulointerstitial fibrosis." in: International journal of molecular medicine, Vol. 37, Issue 5, pp. 1317-27, (2016) (PubMed).

Wu, Zhao, Cheng, Yang, Huang, Meng, Wu, Zhang, Lv, Li: "Melittin induces PTCH1 expression by down-regulating MeCP2 in human hepatocellular carcinoma SMMC-7721 cells." in: Toxicology and applied pharmacology, Vol. 288, Issue 1, pp. 74-83, (2015) (PubMed).

Ma, Li, Li, Yang, Huang, Meng, Zhang, Li: "Geniposide alleviates inflammation by suppressing MeCP2 in mice with carbon tetrachloride-induced acute liver injury and LPS-treated THP-1 cells." in: International immunopharmacology, (2015) (PubMed).

Fan, Wang, Zhao, Liu, Chen, Sun, Zheng: "Sonic hedgehog signaling may promote invasion and metastasis of oral squamous cell carcinoma by activating MMP-9 and E-cadherin expression." in: Medical oncology (Northwood, London, England), Vol. 31, Issue 7, pp. 41, (2014) (PubMed).

Weiss, Guo, Shan, Shi, Romano, Sinha, Cantley, Guo: "Brg1 determines urothelial cell fate during ureter development." in: Journal of the American Society of Nephrology : JASN, Vol. 24, Issue 4, pp. 618-26, (2013) (PubMed).

Du, Feng, Wang, Piao, Cui, Chen, Lei, Sun, Liu, Wang, Li, Yang, Sun, Zhao, Jiang, Li, Jiang: "Curcumin suppresses malignant glioma cells growth and induces apoptosis by inhibition of SHH/GLI1 signaling pathway in vitro and vivo." in: CNS neuroscience & therapeutics, Vol. 19, Issue 12, pp. 926-36, (2013) (PubMed).
抗原

Sonic Hedgehog (SHH)

别名

Shh (SHH 产品)

别名

HHG1 antibody, HLP3 antibody, HPE3 antibody, MCOPCB5 antibody, SMMCI antibody, TPT antibody, TPTPS antibody, 9530036O11Rik antibody, Dsh antibody, Hhg1 antibody, Hx antibody, Hxl3 antibody, M100081 antibody, fc83d08 antibody, shh antibody, syu antibody, vhh-1 antibody, vhh1 antibody, wu:fc83d08 antibody, Xhh antibody, hedgehog antibody, xshh antibody, SHH antibody, twh antibody, twhh antibody, sonic hedgehog antibody, sonic hedgehog a antibody, sonic hedgehog L homeolog antibody, sonic hedgehog protein A antibody, sonic hedgehog b antibody, SHH antibody, Shh antibody, shha antibody, shh.L antibody, shh antibody, shhb antibody

背景

Synonyms: TPT, HHG1, HLP3, HPE3, SMMCI, TPTPS, MCOPCB5, Sonic hedgehog protein, SHH, HHG-1
Background: Intercellular signal essential for a variety of patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites, and the polarizing signal for patterning of the anterior-posterior axis of the developing limb bud. Displays both floor plate- and motor neuron-inducing activity. The threshold concentration of N-product required for motor neuron induction is 5-fold lower than that required for floor plate induction. Activates the transcription of target genes by interacting with its receptor PTCH1 to prevent normal inhibition by PTCH1 on the constitutive signaling activity of SMO (By similarity).

基因ID

6469

UniProt

Q15465

途径

Hedgehog Signaling, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development