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GFP 抗体 (AA 25-120) (HRP)

Name: GFP 抗体 (AA 25-120) (HRP)
SKU: ABIN729442
Availability: OutOfStock

GFP 适用: Aequorea victoria, Aequorea coerulescens (Belt Jellyfish) WB, ELISA, IHC (fro), IHC (p) 宿主: 兔 Polyclonal HRP

产品编号 ABIN729442

发货至: 中国

Datasheet as PDF

抗原 See all GFP 抗体

GFP (Green Fluorescent Protein (GFP))
抗原表位
16

14

10

8

4

4

3

3

2

2

2

1

1

1

1

1

1

1
AA 25-120
适用
174

14

11

9
Aequorea victoria, Aequorea coerulescens (Belt Jellyfish)
宿主
64

62

34

13

9

5

2

1
兔
Compatible Secondaries
克隆类型
112

73
多克隆
标记
82

12

11

9

5

4

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This GFP antibody is conjugated to HRP
应用范围
147

79

47

34

27

25

23

21

13

13

13

12

11

5

4

4

4

2

1

1

1

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

交叉反应

Pig, 标记

交叉反应（详细）

Aequorea victoria, Aequorea coerulescens (belt jellyfish)

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic peptide derived from jellyfish Green Fluorecent protein

亚型

IgG

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GFP 适用: Aequorea victoria WB, IF, IHC (fro) 宿主: 山羊 Polyclonal DyLight 550

应用备注

WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

限制

仅限研究用
状态

Liquid

浓度

1 μg/μL

缓冲液

Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

注意事项

Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

储存条件

-20 °C

储存方法

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

有效期

12 months
抗原

GFP (Green Fluorescent Protein (GFP))

别名

GFP (GFP 产品)

背景

Synonyms: Green fluorescence protein, GRP, GFP TAG, GFP-TAG.
Background: Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology.

UniProt

P42212