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Nanog 抗体 (AA 71-170)

Name: Nanog 抗体 (AA 71-170)
SKU: ABIN728758
Availability: OutOfStock

NANOG 适用: 人, 小鼠 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

Lane 1: A549 cell lysates probed with Rabbit Anti-Nanog Polyclonal Antibody, Unconjugated at 1:5000 for 90 min at 37˚C. (Nanog 抗体 (AA 71-170))

Formalin-fixed and paraffin embedded mouse tooth germ tissue labeled with Anti-Nanog Polyclonal Antibody (ABIN728758), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining (Nanog 抗体 (AA 71-170))

IHC

Formalin-fixed and paraffin embedded mouse tooth germ tissue labeled with Anti-Nanog Polyclonal Antibody , Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining (Nanog 抗体 (AA 71-170))

IHC (p)

HeLa cells probed with Estrogen Receptor alpha + beta Antibody, unconjugated at 1:100 dilution for 30 minutes compared to control cells (blue) and isotype control (orange) (Nanog 抗体 (AA 71-170))

4 images

(3 references)

产品编号 ABIN728758

发货至: 中国

Datasheet as PDF

抗原 See all Nanog (NANOG) 抗体

Nanog (NANOG) (Nanog Homeobox (NANOG))
抗原表位
16

15

12

10

8

7

7

7

6

5

3

3

3

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 71-170
适用
137

57

22

4

4

4

3

3

2

2

1

1

1
人, 小鼠
宿主
126

16

3

1
兔
Compatible Secondaries
克隆类型
121

25
多克隆
标记
75

9

8

8

7

5

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2
This Nanog antibody is un-conjugated
应用范围
108

60

43

43

25

25

21

18

18

14

7

7

5

1

1

1

1

1

1
Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

交叉反应

人, 小鼠

预测反应

Rat,Pig

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic peptide derived from mouse Nanog

亚型

IgG

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anti-Nanog Homeobox (NANOG) antibody
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anti-Nanog Homeobox (NANOG) (AA 101-200) antibody
NANOG 适用: 人, 小鼠, 大鼠 WB, ELISA, FACS, ICC, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Nanog Homeobox (NANOG) (AA 1-305) antibody
NANOG 适用: 人 WB, ELISA, IF, FACS, ICC 宿主: 小鼠 Monoclonal 8A1D11 unconjugated

anti-Nanog Homeobox (NANOG) (AA 15-49) antibody
NANOG 适用: 人 WB, ELISA, IF, IHC, FACS 宿主: 兔 Polyclonal unconjugated

anti-Nanog Homeobox (NANOG) (AA 98-195) antibody
NANOG 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2C11 unconjugated

anti-Nanog Homeobox (NANOG) (AA 98-195) antibody
NANOG 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3A12 unconjugated

anti-Nanog Homeobox (NANOG) (Internal Region) antibody
Verified NANOG 适用: 人 WB, ELISA, IF, IHC 宿主: 山羊 Polyclonal unconjugated

anti-Nanog Homeobox (NANOG) (AA 1-100) antibody
NANOG 适用: 人 WB 宿主: 兔 Polyclonal unconjugated

anti-Nanog Homeobox (NANOG) (AA 20-166) antibody
NANOG 适用: 人 WB, ELISA, ICC 宿主: 小鼠 Monoclonal 1E6C4 unconjugated

anti-Nanog Homeobox (NANOG) (AA 267-292) antibody
NANOG 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated

应用备注

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

限制

仅限研究用
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
El-Badawy, Ghoneim, Gabr, Salah, Mohamed, Amer, El-Badri: "Cancer cell-soluble factors reprogram mesenchymal stromal cells to slow cycling, chemoresistant cells with a more stem-like state." in: Stem cell research & therapy, Vol. 8, Issue 1, pp. 254, (2017) (PubMed).

Liu, Zhang, Wang, Yang, Tan: "JNK is required for maintaining the tumor-initiating cell-like properties of acquired chemoresistant human cancer cells." in: Acta pharmacologica Sinica, Vol. 36, Issue 9, pp. 1099-106, (2016) (PubMed).

Liu, Li, Liu, Sui, Liu, Xiao, Zheng, Chen, Yao, Xing, Zhou, Li: "A signature for induced pluripotent stem cell-associated genes in colorectal cancer." in: Medical oncology (Northwood, London, England), Vol. 30, Issue 1, pp. 426, (2013) (PubMed).
抗原

Nanog (NANOG) (Nanog Homeobox (NANOG))

别名

Nanog (NANOG 产品)

别名

2410002E02Rik antibody, ENK antibody, ecat4 antibody, NANOG antibody, hacp antibody, wu:fd19e04 antibody, wu:fd20a07 antibody, zgc:193933 antibody, Nanog homeobox antibody, nanog homeobox antibody, Nanog antibody, NANOG antibody, nanog antibody

背景

Synonyms: ENK, ecat4, 2412E2Rik, Homeobox protein NANOG, ES cell-associated protein 4, Early embryo specific expression NK-type homeobox protein, Homeobox transcription factor Nanog, Nanog
Background: Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation.

基因ID

71950

UniProt

Q80Z64

途径

Stem Cell Maintenance