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GFAP 抗体 (AA 51-150)

Name: GFAP 抗体 (AA 51-150)
SKU: ABIN726200
Availability: OutOfStock

GFAP 适用: 人, 大鼠, 小鼠 WB, ELISA, FACS, ICC, IHC (p), IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated

U251 Cells lysates probed with GFAP Polyclonal Antibody, unconjugated at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37°C. (GFAP 抗体 (AA 51-150))

Image provided by One World Lab validation program. (GFAP 抗体 (AA 51-150))

IF (cc)

Formalin-fixed and paraffin embedded: rat brain tissue Anti-GFAP Polyclonal Antibody, Unconjugated at 1:400, followed by conjugation to the secondary antibody and DAB staining (GFAP 抗体 (AA 51-150))

IHC (p)

Paraformaldehyde-fixed, paraffin embedded rat brain, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30min, Antibody incubation with GFAP Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by a conjugated secondary -AF594) at 1:500 for 90 minutes and DAPI staining of the nuclei. (GFAP 抗体 (AA 51-150))

8 images

(20 references)

产品编号 ABIN726200

发货至: 中国

Datasheet as PDF

抗原 See all GFAP 抗体

GFAP (Glial Fibrillary Acidic Protein (GFAP))
抗原表位
30

27

26

15

12

9

9

9

8

7

7

6

4

4

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 51-150
适用
293

172

171

50

50

33

30

11

10

9

7

6

5

4

3

2

2

2

1

1

1

1

1
人, 大鼠, 小鼠
宿主
192

142

12

3

2

2
兔
Compatible Secondaries
克隆类型
197

156
多克隆
标记
164

27

23

16

11

7

7

4

4

4

4

4

4

4

4

4

4

4

4

4

4

4

4

4

4

3

2

2

2

2

2

2

2

2

2

2

1

1

1
This GFAP antibody is un-conjugated
应用范围
270

127

91

79

72

58

46

28

25

25

22

17

13

8

8

4

4

3

2

2

1

1

1

1

1

1
Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

交叉反应

人, 小鼠, 大鼠

预测反应

Dog,Cow,Sheep,Pig,Rabbit

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic peptide derived from human GFAP

亚型

IgG
anti-Glial Fibrillary Acidic Protein (GFAP) (C-Term) antibody
GFAP 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Glial Fibrillary Acidic Protein (GFAP) (AA 93-432) antibody
GFAP 适用: 人, 大鼠, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Glial Fibrillary Acidic Protein (GFAP) (AA 253-354) antibody
GFAP 适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated

anti-Glial Fibrillary Acidic Protein (GFAP) (AA 1-430) antibody
GFAP 适用: 大鼠 WB, IHC, ICC, IP 宿主: 小鼠 Monoclonal C9 unconjugated

anti-Glial Fibrillary Acidic Protein (GFAP) (AA 292-432) antibody
GFAP 适用: 人 WB, IHC, IF, ELISA, FACS 宿主: 小鼠 Monoclonal 1C91F1 unconjugated

anti-Glial Fibrillary Acidic Protein (GFAP) antibody
GFAP 适用: 大鼠, 小鼠 WB, IF, IHC (p) 宿主: 小鼠 Monoclonal unconjugated

anti-Glial Fibrillary Acidic Protein (GFAP) (pSer38) antibody
GFAP 适用: 人, 大鼠, 小鼠 WB, IHC, IF, ELISA, ICC 宿主: 兔 Polyclonal unconjugated

anti-Glial Fibrillary Acidic Protein (GFAP) (C-Term) antibody
Verified GFAP 适用: 人, 大鼠, 小鼠 WB, ELISA 宿主: 山羊 Polyclonal unconjugated

anti-Glial Fibrillary Acidic Protein (GFAP) antibody
GFAP 适用: 人 WB, IHC, IF 宿主: 兔 Monoclonal unconjugated

anti-Glial Fibrillary Acidic Protein (GFAP) (AA 108-211) antibody
GFAP 适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated

应用备注

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

限制

仅限研究用
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
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抗原

GFAP (Glial Fibrillary Acidic Protein (GFAP))

别名

GFAP (GFAP 产品)

别名

GFAP antibody, AI836096 antibody, cb345 antibody, etID36982.3 antibody, gfapl antibody, wu:fb34h11 antibody, wu:fk42c12 antibody, xx:af506734 antibody, zgc:110485 antibody, glial fibrillary acidic protein antibody, GFAP antibody, LOC100136168 antibody, gfap antibody, Gfap antibody

背景

Synonyms: Glial fibrillary acidic protein, GFAP
Background: GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.

基因ID

2670

UniProt

P14136