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GFAP 抗体 (AA 51-150)

Name: GFAP 抗体 (AA 51-150)
SKU: ABIN726200
Availability: OutOfStock

GFAP 适用: 人, 大鼠, 小鼠 WB, ELISA, FACS, IHC (p), ICC, IHC (fro), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated

U251 Cells lysates probed with GFAP Polyclonal Antibody, unconjugated at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37°C. (GFAP 抗体 (AA 51-150))

Image provided by One World Lab validation program. (GFAP 抗体 (AA 51-150))

IF (cc)

Formalin-fixed and paraffin embedded: rat brain tissue Anti-GFAP Polyclonal Antibody, Unconjugated at 1:400, followed by conjugation to the secondary antibody and DAB staining (GFAP 抗体 (AA 51-150))

IHC (p)

Paraformaldehyde-fixed, paraffin embedded rat brain, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30min, Antibody incubation with GFAP Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by a conjugated secondary -AF594) at 1:500 for 90 minutes and DAPI staining of the nuclei. (GFAP 抗体 (AA 51-150))

8 images

(20 references)

产品编号 ABIN726200

发货至: 中国

Datasheet as PDF

抗原 See all GFAP 抗体

GFAP (Glial Fibrillary Acidic Protein (GFAP))
抗原表位
30

27

20

15

12

9

9

8

8

7

7

6

4

4

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 51-150
适用
285

171

169

51

51

34

31

11

11

10

7

6

5

4

3

2

2

2

1

1

1

1
人, 大鼠, 小鼠
宿主
189

137

12

2

2

2
兔
Compatible Secondaries
克隆类型
189

154

1
多克隆
标记
167

26

23

16

10

6

6

4

4

4

4

4

4

4

4

4

4

4

4

3

3

3

3

3

3

3

2

2

2

2

2

2

2

2

2

1

1

1
This GFAP antibody is un-conjugated
应用范围
268

126

89

78

74

58

46

28

25

25

22

18

13

8

8

4

4

3

2

2

2

1

1

1

1

1

1
Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc))

交叉反应

人, 小鼠, 大鼠

预测反应

Dog,Cow,Sheep,Pig,Rabbit

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic peptide derived from human GFAP

亚型

IgG
anti-Glial Fibrillary Acidic Protein (GFAP) (C-Term) antibody
GFAP 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Glial Fibrillary Acidic Protein (GFAP) (AA 93-432) antibody
GFAP 适用: 人, 大鼠, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Glial Fibrillary Acidic Protein (GFAP) (AA 253-354) antibody
GFAP 适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated

anti-Glial Fibrillary Acidic Protein (GFAP) (AA 1-430) antibody
GFAP 适用: 大鼠 WB, IHC, ICC, IP 宿主: 小鼠 Monoclonal C9 unconjugated

anti-Glial Fibrillary Acidic Protein (GFAP) (AA 292-432) antibody
GFAP 适用: 人 WB, IHC, IF, ELISA, FACS 宿主: 小鼠 Monoclonal 1C91F1 unconjugated

anti-Glial Fibrillary Acidic Protein (GFAP) (pSer38) antibody
GFAP 适用: 人, 大鼠, 小鼠 WB, IHC, IF, ELISA, ICC 宿主: 兔 Polyclonal unconjugated

anti-Glial Fibrillary Acidic Protein (GFAP) (C-Term) antibody
Verified GFAP 适用: 人, 大鼠, 小鼠 WB, ELISA 宿主: 山羊 Polyclonal unconjugated

anti-Glial Fibrillary Acidic Protein (GFAP) antibody
GFAP 适用: 人 WB, IHC, IF 宿主: 兔 Monoclonal unconjugated

anti-Glial Fibrillary Acidic Protein (GFAP) (AA 108-211) antibody
GFAP 适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated

anti-Glial Fibrillary Acidic Protein (GFAP) antibody
GFAP 适用: 人, 大鼠, 小鼠, Pig, Cow, 小鸡, 兔 WB, IHC, IF, FACS, StM 宿主: 小鼠 Monoclonal GA-5 unconjugated

应用备注

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

限制

仅限研究用
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
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抗原

GFAP (Glial Fibrillary Acidic Protein (GFAP))

别名

GFAP (GFAP 产品)

背景

Synonyms: Glial fibrillary acidic protein, GFAP
Background: GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.

基因ID

2670

UniProt

P14136