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AKT 1/2/3 抗体 (AA 401-479)

Name: AKT 1/2/3 抗体 (AA 401-479)
SKU: ABIN725195
Availability: OutOfStock
Rating: 5 (1 reviews)

适用: 人, 小鼠, 大鼠, Cow, Pig WB, IF (cc), IF (p), FACS, ELISA, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

Flow Cytometry validation image for anti-AKT 1/2/3 (AA 401-479) antibody (ABIN725195) (AKT 1/2/3 抗体 (AA 401-479))

FACS

Mouse placenta lysate with Anti-AKT1/3 Polyclonal Antibody Unconjugated at 1:5000 for 90 min at 37˚C. (AKT 1/2/3 抗体 (AA 401-479))

Formalin-fixed and paraffin embedded: mouse transplant lymphoma labeled with Anti-PKB Polyclonal Antibody, Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining (AKT 1/2/3 抗体 (AA 401-479))

IHC

Lane 1: Mouse Placenta lysates；Lane 2: Mouse Lung lysates probed with AKT1/3 Polyclonal Antibody, unconjugated at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37°C. (AKT 1/2/3 抗体 (AA 401-479))

5 images

(9 references)

(1 validation)

产品编号 ABIN725195

发货至: 中国

Datasheet as PDF

抗原 See all AKT 1/2/3 products

AKT 1/2/3
抗原表位
22

21

20

20

19

19

17

16

16

15

7

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 401-479
适用
147

140

126

43

31

26

22

15

7

6

4

4
人, 小鼠, 大鼠, Cow, Pig
宿主
145

2
兔
Compatible Secondaries
克隆类型
135

12
多克隆
标记
35

7

7

6

6

6

6

6

6

6

6

6

6

6

6

6

2

2

2

2

2

2

2

2

2

2
This AKT 1/2/3 antibody is un-conjugated
应用范围
127

78

77

76

32

26

25

17

7

7

6
Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Flow Cytometry (FACS), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

交叉反应

Cow, 人, 小鼠, Pig, 大鼠

预测反应

Dog,Cow,Sheep,Chicken

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic peptide derived from human AKT1

亚型

IgG
anti-AKT 1/2/3 (pSer473) antibody
适用: 人, 小鼠, 大鼠, Cow, 兔, 小鸡, 鱼 WB, IF (cc), IF (p), ELISA, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-AKT 1/2/3 antibody
适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated

anti-AKT 1/2/3 (pSer472) antibody
适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-AKT 1/2/3 (pTyr312), (pTyr315), (pTyr316) antibody
适用: 人, 小鼠, 大鼠 WB, IF (cc), IF (p), FACS, ELISA, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-AKT 1/2/3 (pTyr474) antibody
适用: 人, 小鼠 WB, IF (cc), IF (p), FACS, ELISA, IHC (p), IHC (fro), ICC 宿主: 兔 Polyclonal unconjugated

anti-AKT 1/2/3 (pTyr312), (pTyr315), (pTyr316) antibody
适用: 人, 小鼠, 大鼠 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-AKT 1/2/3 (pThr305), (pThr308), (pThr309) antibody
适用: 人, 小鼠, 大鼠 WB, IF (cc), IF (p), FACS, ELISA, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-AKT 1/2/3 (AA 278-312) antibody
适用: 人, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB43841 unconjugated

anti-AKT 1/2/3 (AA 431-480) antibody
适用: 人, 小鼠, 大鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated

anti-AKT 1/2/3 (pSer472) antibody
适用: 人 WB 宿主: 兔 Monoclonal unconjugated

应用备注

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

限制

仅限研究用
生效 #029769 (Flow Cytometry)

by

Flow Cytometry & Cell Separation Facility, Purdue University

No.

#029769

日期

2014.07.22

抗原

Lot Number

131113

Method validated

Flow Cytometry

Positive Control

MDA-MB-468 cells - lower expression

Negative Control

Notes

A strong specific signal is observed in both of the positive control cells stained with anti-AKT1 plus secondary antibody compared with isotype, secondary only and unstained cells.

Validation Images

Figure 1: Positive control (high expression) MCF7 cells. The red histogram is unstained cells, the blue histogram is cells stained with secondary antibody alone, the green histogram is cells stained with rabbit IgG isotype control antibody plus secondary antibody and the orange histogram is cells stained with anti-AKT1 plus secondary antibody. The secondary antibody is a goat anti-rabbit IgG-FITC (Jackson Immunoresearch).

Figure 2: Positive control (lower expression) MDA-MB-468 cells. The red histogram is unstained cells, the blue histogram is cells stained with secondary antibody alone, the green histogram is cells stained with rabbit IgG isotype control antibody plus secondary antibody and the orange histogram is cells stained with anti-AKT1 plus secondary antibody. The secondary antibody is a goat anti-rabbit IgG-FITC (Jackson Immunoresearch).
Full Methods
Primary Antibody

Antigen: V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1)
Catalog number: ABIN725195
Lot number: 131113
Dilution: 1 µg in 100 µL 1X PBS containing 0.5% BSA

Secondary Antibody

Antibody: Goat anti-rabbit IgG-FITC
Lot number: 115629
Dilution: 1:200 in 1X PBS containing 0.5% BSA

Full Protocol

Control cells were cultured in DMEM + 10% FBS. - Control cells were washed once with phosphate-buffered saline (PBS) and harvested with a non-enzymatic cell dissociation solution (Cellstripper, Mediatech, Inc).
Detached cells were washed and resuspended in 0.5 mL 1X PBS containing 0.5% BSA.
An equivalent amount of pre-warmed 4% paraformaldehyde was added and the cells were incubated for 10 min at 37 °C.
Cells were washed in 1X PBS containing 0.5% BSA and resuspended in 1 mL of ice-cold 90% methanol for 30 min on ice.
Cells were spun out of the methanol and washed twice by resuspending in 1X PBS containing 0.5% BSA.
Cells were resuspended in 100 µL 1X PBS containing 0.5% BSA: - unstained cells - secondary antibody alone - isotype control antibody + secondary antibody - primary antibody + secondary antibody
Cells were incubated for 30 min on ice.
Labeled cells were washed twice in PBS containing 0.5% BSA.
Cells were resuspended with 1X PBS containing 0.5% BSA + 10% goat serum and incubated for 15 min at room temperature.
Goat anti-rabbit IgG-FITC secondary antibody (Jackson Immunoresearch) was added at a 1:200 dilution. The cells were incubated for 30 min in the dark on ice.
Labeled cells were washed twice in PBS containing 0.5% BSA.
Cells were analyzed on a FACSAria III (BD Biosciences) using a blue laser (488 nm excitation / 525 nm emission).

Experimental Notes

Nothing to note.
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
Liu, Liu, Liu, Ma, Zhao: "Surgical incision induces learning impairment in mice partially through inhibition of the brain-derived neurotrophic factor signaling pathway in the hippocampus and amygdala." in: Molecular pain, Vol. 14, pp. 1744806918805902, (2019) (PubMed).

Zhang, Zhao, Zhang, Hao, Yu, Min, Li, Ma, Chen, Yi, Tang, Meng, Liu, Wang, Shen, Zhang: "Decrease in male mouse fertility by hydrogen sulfide and/or ammonia can Be inheritable." in: Chemosphere, Vol. 194, pp. 147-157, (2018) (PubMed).

Zhao, Zhang, Liu, Zhang, Hao, Li, Chen, Shen, Tang, Min, Meng, Wang, Yi, Zhang: "Hydrogen Sulfide and/or Ammonia Reduces Spermatozoa Motility through AMPK/AKT Related Pathways." in: Scientific reports, Vol. 6, pp. 37884, (2016) (PubMed).

Zhang, Huang, Guo, Gou, Hinsdale, Lloyd, Liu: "MicroRNA-26b Modulates the NF-κB Pathway in Alveolar Macrophages by Regulating PTEN." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 195, Issue 11, pp. 5404-14, (2015) (PubMed).

Liang, Zeng, Wang, Shen, Li, Ma, Ci, Yu, Jia, Sun, Liu, Liu, Li, Yu, Chen, Jia: "Histone demethylase RBP2 induced by Helicobactor Pylori CagA participates in the malignant transformation of gastric epithelial cells." in: Oncotarget, Vol. 5, Issue 14, pp. 5798-807, (2015) (PubMed).

Li, Liu, Wang, Sun, Ding, Sun, Han, Wang: "Follistatin could promote the proliferation of duck primary myoblasts by activating PI3K/Akt/mTOR signalling." in: Bioscience reports, Vol. 34, Issue 5, (2014) (PubMed).

Xu, Huang, Xie, Lin, Guo, Zhuang, Lin, Zhou, Li, Huang, Wei, Man, Zhang: "The expression of cytoglobin as a prognostic factor in gliomas: a retrospective analysis of 88 patients." in: BMC cancer, Vol. 13, pp. 247, (2013) (PubMed).

Tang, Liu, Dong, Li, Li, Lin, Zheng, Hou, Li: "The cardioprotective effects of citric Acid and L-malic Acid on myocardial ischemia/reperfusion injury." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2013, pp. 820695, (2013) (PubMed).
抗原

AKT 1/2/3

别名

AKT1/2/3 (AKT 1/2/3 产品)

背景

Synonyms: AKT, PKB, RAC, CWS6, PRKBA, PKB-ALPHA, RAC-ALPHA, RAC-alpha serine/threonine-protein kinase, Protein kinase B, Protein kinase B alpha, PKB alpha, Proto-oncogene c-Akt, RAC-PK-alpha, AKT1, RAC-gamma serine/threonine-protein kinase, Protein kinase Akt-3, Protein kinase B gamma, PKB gamma, RAC-PK-gamma, STK-2, PKBG
Background: AKT1-specific substrates have been recently identified, including palladin (PALLD), which phosphorylation modulates cytoskeletal organization and cell motility, prohibitin (PHB), playing an important role in cell metabolism and proliferation, and CDKN1A, for which phosphorylation at 'Thr-145' induces its release from CDK2 and cytoplasmic relocalization. These recent findings indicate that the AKT1 isoform has a more specific role in cell motility and proliferation. Phosphorylates CLK2 thereby controlling cell survival to ionizing radiation.

基因ID

207

UniProt

P31749