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Leptin 抗体 (AA 101-154)

Name: Leptin 抗体 (AA 101-154)
SKU: ABIN725030
Availability: OutOfStock

LEP 适用: 小鼠, 大鼠 ELISA, FACS, IF (p), IF (cc), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

Formalin-fixed and paraffin embedded: rat liver labeled with Anti-Leptin Polyclonal Antibody (ABIN725030), Unconjugated 1:300 followed by conjugation to the secondary antibody and DAB staining (Leptin 抗体 (AA 101-154))

IHC

Paraformaldehyde-fixed, paraffin embedded rat brain, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Leptin Polyclonal Antibody, Unconjugated at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining. (Leptin 抗体 (AA 101-154))

IHC (p)

Paraformaldehyde-fixed, paraffin embedded mouse liver, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Leptin Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining. (Leptin 抗体 (AA 101-154))

IHC (p)

3 images

产品编号 ABIN725030

发货至: 中国

Datasheet as PDF

抗原 See all Leptin (LEP) 抗体

Leptin (LEP)
抗原表位
25

15

15

15

12

12

7

5

4

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 101-154
适用
122

73

71

12

8

7

5

3

3

3

2

1

1

1
小鼠, 大鼠
宿主
161

29

8

5

1
兔
Compatible Secondaries
克隆类型
173

30
多克隆
标记
98

24

16

8

8

7

3

3

3

3

3

3

3

3

3

3

3

3

1

1

1

1

1

1

1
This Leptin antibody is un-conjugated
应用范围
127

83

59

38

38

37

32

30

22

18

15

11

10

7

5

4

2

2

2

1

1

1

1

1

1
ELISA, Flow Cytometry (FACS), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

交叉反应

小鼠, 大鼠

预测反应

Dog,Cow,Pig,Horse

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic peptide derived from mouse Leptin

亚型

IgG

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anti-Leptin (LEP) (AA 22-167) antibody
LEP 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Leptin (LEP) (AA 17-100) antibody
LEP 适用: 人, 小鼠, 大鼠 WB, ELISA, IF (p), IF (cc), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Leptin (LEP) (AA 8-37) antibody
LEP 适用: 人 WB, ELISA, IHC, IF, FACS 宿主: 兔 Polyclonal unconjugated

anti-Leptin (LEP) (AA 22-167) antibody
LEP 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Leptin (LEP) (AA 29-160) antibody
LEP 适用: Cow WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Leptin (LEP) (AA 22-167) antibody
LEP 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated

anti-Leptin (LEP) (AA 7-37), (N-Term) antibody
LEP 适用: 人 WB, IF, FACS, EIA, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Leptin (LEP) (AA 22-167) antibody
LEP 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C5 unconjugated

anti-Leptin (LEP) (AA 22-167) antibody
LEP 适用: 犬 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

应用备注

ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

限制

仅限研究用
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
抗原

Leptin (LEP)

别名

Leptin (LEP 产品)

别名

ob antibody, obese antibody, LEPD antibody, OB antibody, OBS antibody, leptin antibody, Lep antibody, LEP antibody, lep antibody

背景

Synonyms: ob, obese, Leptin, Obesity factor, Lep
Background: May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass.

基因ID

16846

UniProt

P41160

途径

JAK/STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process