CH25H 抗体 (C-Term)
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- 抗原 See all CH25H 抗体
- CH25H (Cholesterol 25-Hydroxylase (CH25H))
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抗原表位
- C-Term
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This CH25H antibody is un-conjugated
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应用范围
- ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
- 原理
- CH25H Antibody
- 交叉反应 （详细）
- This affinity purified antibody is directed against human CH25H.
- 产品特性
- Synonyms: Rabbit Anti-Cholesterol 25-Hydroxylase, Rabbit Anti-CH25H, Cholesterol 25-Monooxygenase, EC 1.14.99.38, H25OH, C25H
- 纯化方法
- This affinity purified antibody is directed against human CH25H.
- 过滤
- Sterile filtered
- 免疫原
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Immunogen: Anti-CH25H antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Terminal region of human CH25H conjugated to Keyhole Limpet Hemocyanin (KLH).
Immunogen Type: Conjugated Peptide
- Top Product
- Discover our top product CH25H Primary Antibody
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anti-Cholesterol 25-Hydroxylase (CH25H) (AA 1-100) antibody
CH25H 适用: 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Cholesterol 25-Hydroxylase (CH25H) (AA 142-272) antibodyCH25H 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Cholesterol 25-Hydroxylase (CH25H) (AA 206-272) antibodyCH25H 适用: 人 ELISA, WB 宿主: 小鼠 Polyclonal unconjugated
anti-Cholesterol 25-Hydroxylase (CH25H) (AA 190-239) antibodyCH25H 适用: 人, 猴 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Cholesterol 25-Hydroxylase (CH25H) (AA 47-76) antibodyCH25H 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal RB55304 unconjugated
anti-Cholesterol 25-Hydroxylase (CH25H) (AA 142-247) antibodyCH25H 适用: 人 ELISA, WB 宿主: 小鼠 Monoclonal 1G8 unconjugated
anti-Cholesterol 25-Hydroxylase (CH25H) (C-Term) antibodyCH25H 适用: 人, 小鼠, Cow, 山羊, 豚鼠, 马, 兔, 大鼠, Saccharomyces cerevisiae, 斑马鱼 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Application Note: Anti-CH25H Antibody has been tested in ELISA, WB, and IHC. Expect a band at ~31.7 kDa in western blot using appropriate tissues or lysates. Positive control used: Human Kidney Tissues in Immunohistochemistry. Immunohistochemistry Dilution: 1:100 Western Blot Dilution: 1:1,000 ELISA Dilution: 1:10,000 - 1:50,000
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.92 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
- 有效期
- 12 months
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- 抗原
- CH25H (Cholesterol 25-Hydroxylase (CH25H))
- 别名
- CH25H (CH25H 产品)
- 背景
- Background: CH25H (Cholesterol 25-Hydroxylase catalyzes the formation of 25-hydroxycholesterol from cholesterol, leading to repress cholesterol biosynthetic enzymes. CH25H plays a key role in cell positioning and movement in lymphoid tissues: 25-hydroxycholesterol is an intermediate in biosynthesis of 7-alpha,25-dihydroxycholesterol (7-alpha,25-OHC), an oxysterol that acts as a ligand for the G protein-coupled receptor GPR183/EBI2, a chemotactic receptor for several lymphoid cells. It may play an important role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein (SREBP) processing. In testis, production of 25-hydroxycholesterol by macrophages may play a role in Leydig cell differentiation. Anti-CH25H Antibody is useful for researchers interested in Alzheimer's Disease, Neuroscience, and metabolism.
- 基因ID
- 9023
- NCBI登录号
- NP_003947
- UniProt
- O95992
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