COL1A1/A2 抗体
适用: 哺乳动物
DB, ELISA, FLISA, FACS, FM, IHC, IP, WB
宿主: 兔
Polyclonal
unconjugated
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- 抗原
- COL1A1/A2
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适用
- 哺乳动物
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- 非结合性
- 应用范围
- Dot Blot (DB), ELISA, FLISA, Flow Cytometry (FACS), Fluorescence Microscopy (FM), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
- 原理
- Collagen Type I Antibody
- 交叉反应 （详细）
- Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues.
- 产品特性
- Synonyms: rabbit anti-collagen type I antibody, Collagen Of Skin Tendon And Bone, Collagen Type 1 antibody, Collagen type I alpha 1 antibody, Collagen alpha-1 (I) chain, Alpha-1 type I collagen, type 1 procollagen alpha 1
- 纯化方法
- COLLAGEN I Antibody has been prepared by immunoaffinity chromatography using immobilized antigens.
- 过滤
- Sterile filtered
- 免疫原
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Immunogen: Collagen Type I from human and bovine placenta
Immunogen Type: Native Protein
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anti-COL1A1/A2 antibody
适用: Cow, 人, 小鼠, Pig, 大鼠 DB, ELISA, FLISA, FACS, FM, IHC, IP, WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Application Note: Anti-Collagen Type I has been tested by Western blot, dot blot, and IHC and is suitable for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, IP, native PAGE, immunofluorescence, and FC for highly sensitive qualitative analysis. Flow Cytometry Dilution: User Optimized Immunohistochemistry Dilution: 1:50 - 1:200 Western Blot Dilution: 1:1,000 - 1:10,000 Immunoprecipitation Dilution: 1:100 FLISA Dilution: 1:100 ELISA Dilution: 1:5,000 - 1:50,000 IF Microscopy Dilution: User Optimized Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
- 有效期
- 12 months
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- 抗原
- COL1A1/A2
- 背景
- Background: Rockland produces highly active antibodies and conjugates to collagens. Collagens are highly conserved throughout evolution and are characterized by an uninterrupted ''Glycine-X-Y'' triplet repeat that is a necessary part of the triple helical structure. For these reasons, it is often extremely difficult to generate antibodies with specificities to collagens. The development of 'type' specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Rockland extensively purifies collagens for immunization from human and bovine placenta and cartilage by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions are used for SDS-PAGE and immunoblotting. Collagen Type I is a protein that strengthens and supports many tissues in the body, including cartilage, bone, tendon, skin and the white part of the eye (sclera). Collagen Type I triple helix comprises of two alpha1 chains and one alpha2 chain. COL1A1/A2 could be useful for detecting melanoma, lung, liver, glioma, skin, stomach, and other cancers. Mutations in the gene may be related to caffey disease, osteogenesis, and ehlers-danlos syndrome. Anti-Collagen Type I Antibody is ideal for investigators involved in extracellular matrix protein, osteoporosis research, Cell Biology, Signal Transduction, and Stem Cell research.
- 基因ID
- 1277
- NCBI登录号
- NP_000079
- UniProt
- P02452
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