PRC1 抗体 (HRP)
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- 抗原 See all PRC1 抗体
- PRC1 (Protein Regulator of Cytokinesis 1 (PRC1))
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适用
- Saccharomyces cerevisiae
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This PRC1 antibody is conjugated to HRP
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
- 原理
- Carboxypeptidase Y Antibody Peroxidase Conjugated
- 交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Carboxypeptidase Y [Baker's Yeast].
- 产品特性
- Synonyms: rabbit anti-Carboxypeptidase Y antibody peroxidase conjugation, HRP conjugated rabbit anti-Carboxypeptidase Y Antibody, Carboxypeptidase YSCY antibody, CPY1 antibody, LBC1 antibody, PRC1 antibody, Vacuolar carboxypeptidase Y antibody, YMR297W antibody, Carboxypeptidase C
- 纯化方法
- Carboxypeptidase Y is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
- 过滤
- Sterile filtered
- 免疫原
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Immunogen: Carboxypeptidase Y [Baker's Yeast]
Immunogen Type: Native Protein
- Top Product
- Discover our top product PRC1 Primary Antibody
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anti-Protein Regulator of Cytokinesis 1 (PRC1) (AA 351-620) antibody
PRC1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Protein Regulator of Cytokinesis 1 (PRC1) (AA 1-620) antibodyPRC1 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 3B10-1F9 unconjugated
anti-Protein Regulator of Cytokinesis 1 (PRC1) (Internal Region) antibodyPRC1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Protein Regulator of Cytokinesis 1 (PRC1) (pThr481) antibodyPRC1 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Protein Regulator of Cytokinesis 1 (PRC1) (AA 460-520) antibodyPRC1 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Protein Regulator of Cytokinesis 1 (PRC1) (AA 1-150) antibodyPRC1 适用: 人 WB 宿主: 小鼠 Monoclonal 6G2 unconjugated
anti-Protein Regulator of Cytokinesis 1 (PRC1) (Middle Region) antibodyPRC1 适用: 人, 小鼠, 大鼠 WB, IF, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Application Note: Anti-Carboxypeptidase Y Peroxidase antibody has been tested by ELISA and western blot and is assayed against 1.0 μg of Carboxypeptidase Y [Baker's Yeast] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product. Immunohistochemistry Dilution: User Optimized Western Blot Dilution: 1:1,000 - 1:5,000 ELISA Dilution: 1:2,000 - 1:10,000 Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
- 有效期
- 12 months
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- 抗原
- PRC1 (Protein Regulator of Cytokinesis 1 (PRC1))
- 别名
- PRC1 (PRC1 产品)
- 背景
- Background: Carboxypeptidase Y is an enzyme that catalyzes the reaction of the release of a C-terminal amino acid with broad specificity. Carboxypeptidase Y is a carboxypeptidase with optimum activity at pH 4.5-6.0. It is inhibited by diisopropyl fluorophosphate.
- 基因ID
- 855343
- NCBI登录号
- NP_014026
- UniProt
- P00729
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