Ascorbate Oxidase 抗体 (HRP)
AO
适用: Pumpkin
ELISA, WB, IP
宿主: 兔
Polyclonal
HRP
-
- 抗原 See all Ascorbate Oxidase (AO) products
- Ascorbate Oxidase (AO)
-
适用
- Pumpkin
-
宿主
- 兔
-
克隆类型
- 多克隆
-
标记
- This Ascorbate Oxidase antibody is conjugated to HRP
-
应用范围
- ELISA, Western Blotting (WB), Immunoprecipitation (IP)
- 原理
- Ascorbate Oxidase Antibody Peroxidase Conjugated
- 交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum and purified and partially purified Ascorbate Oxidase [cucurbita].
- 产品特性
- Synonyms: rabbit anti-Ascorbate Oxidase Antibody Peroxidase Conjugation, HRP conjugated rabbit anti-Ascorbate Oxidase Antibody, L-ascorbate oxidase, Ascorbase, ASO
- 纯化方法
- Ascorbate Oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
- 过滤
- Sterile filtered
- 免疫原
-
Immunogen: Ascorbate Oxidase [cucurbita]
Immunogen Type: Native Protein
-
-
- 应用备注
- Application Note: Anti-Ascorbate Oxidase Peroxidase Conjugated Antibody has been tested by ELISA and western blot. This product is assayed against 1.0 μg of Alkaline Phosphatase [Calf Intestine] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:11,000 of the reconstitution concentration is suggested for this product. Western Blot Dilution: 1:500 - 1:5,000 Immunoprecipitation Dilution: 1:100 ELISA Dilution: 1:3,000 - 1:10,000 Other: User Optimized
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- 1.0 mg/mL
- 缓冲液
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
- 有效期
- 12 months
-
- 抗原
- Ascorbate Oxidase (AO)
- 别名
- AAO (AO 产品)
- 背景
- Background: Ascorbate Oxidase belongs to the family of oxidoreductases, specifically those acting on diphenols and related substances as donor with oxygen as acceptor. The systematic name of this enzyme class is L-ascorbate:oxygen oxidoreductase.
- UniProt
- P24792
-
You are here:
