Protein G 抗体 (Biotin)
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- 抗原 See all Protein G 抗体
- Protein G
- 适用
- Streptococcus
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Protein G antibody is conjugated to Biotin
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应用范围
- ELISA, Dot Blot (DB)
- 原理
- Protein G Antibody Biotin Conjugated
- 交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Protein G [Streptococcus species].
- 产品特性
- Synonyms: rabbit anti-Protein G Antibody biotin Conjugation, biotin conjugated rabbit anti-Protein G Antibody, Protein G BAC
- 纯化方法
- Anti-Protein G is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
- 过滤
- Sterile filtered
- 免疫原
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Immunogen: Protein G [Streptococcus species]
Immunogen Type: Native Protein
- Top Product
- Discover our top product Protein G Primary Antibody
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anti-Protein G antibody (Biotin)
适用: Streptococcus ELISA, DB 宿主: 兔 Polyclonal Biotin
anti-Protein G antibody (HRP)适用: Streptococcus ELISA, DB 宿主: 兔 Polyclonal HRP
anti-Protein G antibody (FITC)适用: Streptococcus FACS, IM 宿主: 兔 Polyclonal FITC
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- 应用备注
- Application Note: Anti-Protein G Biotin Antibody has been tested by dot blot and is suitable to be assayed against 1.0 μg of Protein G in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:90,000 to 1:350,000 of the reconstitution concentration is suggested for this product. ELISA Dilution: 1:9,000 - 1:35,000
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
- 有效期
- 12 months
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- 抗原
- Protein G
- Abstract
- Protein G 产品
- 背景
- Background: Protein G is a protein that has the property of binging to immunoglobulins. It is a 65- kDa cell surface protein that is commonly used for purifying antibodies through binding to the Fab and Fc regions. Protein G was originally isolated from Streptococcal bacteria. It is similar in properties to Protein A but has unique IgG binding specificities. Native protein G also binds albumin, however Rockland uses recombinant forms of Protein G that only bind to immunoglobulins. Biotin is widely used throughout the biotechnology industry to conjugate proteins for biochemical assays. Biotin's small size typically does not affect the biological activity of protein upon biotinylation. Biotinylated proteins of interest can be enriched from a sample due to highly stable interactions. Biotin conjugated anti-Protein G antibodies are used as an amplifying reagent in immunohistochemistry, microarray assays, ELISAs, blots, and other applications.
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