Peroxidase 抗体 (Biotin)
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- 抗原 See all Peroxidase 抗体
- Peroxidase
- 适用
- Horseradish
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Peroxidase antibody is conjugated to Biotin
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Dot Blot (DB)
- 原理
- Peroxidase (Horseradish) Antibody Biotin Conjugated
- 交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Peroxidase [Horseradish].
- 产品特性
- Synonyms: rabbit anti-Horseradish Peroxidase Antibody Biotin Conjugation, rabbit anti-HRP Antibody Biotin Conjugation, Biotin Conjugated rabbit anti-HRP Antibody, Peroxidase Biotin Conjugated antibody, Anti-Peroxidase BAC Antibody
- 纯化方法
- Anti-Peroxidase (Horseradish) Antibody Biotin Conjugated is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
- 过滤
- Sterile filtered
- 免疫原
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Immunogen: Peroxidase [Horseradish]
Immunogen Type: Native Protein
- Top Product
- Discover our top product Peroxidase Primary Antibody
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anti-Peroxidase antibody
适用: Horseradish WB, ELISA, DB, IP 宿主: 山羊 Polyclonal unconjugated
anti-Peroxidase antibody适用: Horseradish WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Peroxidase antibody适用: Horseradish WB, ELISA, IHC 宿主: 小鼠 Polyclonal unconjugated
anti-Peroxidase antibody适用: Horseradish WB, ELISA, IHC 宿主: 豚鼠 Polyclonal unconjugated
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- 应用备注
- Application Note: Anti-Peroxidase Antibody Biotin Conjugated has been tested by dot blot and is suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency. Immunohistochemistry Dilution: User Optimized Western Blot Dilution: User Optimized
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
- 有效期
- 12 months
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- 抗原
- Peroxidase
- Abstract
- Peroxidase 产品
- 背景
- Background: Biotin is widely used throughout the biotechnology industry to conjugate proteins for biochemical assays. Biotin's small size typically does not affect the biological activity of protein upon biotinylation. Biotinylated proteins of interest can be enriched from a sample due to highly stable interactions. Biotin conjugated anti-Peroxidase antibodies are used as an amplifying reagent in immunohistochemistry, microarray assays, ELISAs, blots, and other applications.
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