Choline Oxidase 抗体 (HRP)
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- 抗原 See all Choline Oxidase products
- Choline Oxidase
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适用
- Alcaligenes
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This Choline Oxidase antibody is conjugated to HRP
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应用范围
- Western Blotting (WB), ELISA, Immunoprecipitation (IP)
- 原理
- Choline Oxidase Antibody Peroxidase Conjugated
- 交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Choline Oxidase [Alcaligenes species].
- 产品特性
- Synonyms: goat anti-Choline Oxidase Antibody HRP Conjugation, Peroxidase Conjugated goat anti-Choline Oxidase Antibody, codA antibody
- 纯化方法
- Anti-Choline oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
- 过滤
- Sterile filtered
- 免疫原
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Immunogen: Choline Oxidase [Alcaligenes species]
Immunogen Type: Native Protein
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anti-Choline Oxidase antibody (Biotin)
适用: Alcaligenes WB, ELISA, IP 宿主: 山羊 Polyclonal Biotin
anti-Choline Oxidase antibody (HRP)适用: Alcaligenes WB, ELISA, IP 宿主: 山羊 Polyclonal HRP
anti-Choline Oxidase antibody适用: Alcaligenes WB, ELISA, IP 宿主: 山羊 Polyclonal unconjugated
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- 应用备注
- Application Note: Anti-Choline oxidase has been tested by ELISA and western blot and is assayed against 1.0 μg of Choline Oxidase [Alcaligenes species] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product. Western Blot Dilution: 1:500 - 1:1,000 Immunoprecipitation Dilution: 1:100 ELISA Dilution: 1:1,000 - 1:2,500 Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
- 有效期
- 12 months
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- 抗原
- Choline Oxidase
- Abstract
- Choline Oxidase 产品
- 背景
- Background: Choline oxidase is an enzyme that catalyzes the chemical reaction (choline + O2 <=> betaine aldehyde + H2O2). Thus, the two substrates of this enzyme are choline and O2, whereas its two products are betaine aldehyde and H2O2. Choline oxidase belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with oxygen as acceptor. This enzyme participates in glycine, serine, and threonine metabolism.
- 基因ID
- 116367
- UniProt
- P16101
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