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Caspase 8 抗体 (AA 411-482)

Name: Caspase 8 抗体 (AA 411-482)
SKU: ABIN724205
Availability: OutOfStock
Rating: 5 (1 reviews)

CASP8 适用: 人, 小鼠, 大鼠, 猴, Pig, 绵羊 WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

Western Blotting validation image for anti-Caspase 8 (CASP8) (AA 411-482) antibody (ABIN724205) (Caspase 8 抗体 (AA 411-482))

Image provided by the Independent Validation Program (badge number 29759). (Caspase 8 抗体 (AA 411-482))

Formalin-fixed and paraffin embedded human rectal carcinoma labeled with Anti-Caspase-8 Polyclonal Antibody, Unconjugated (ABIN724205) 1:200 followed by conjugation to the secondary antibody and DAB staining (Caspase 8 抗体 (AA 411-482))

IHC

L1 mouse liver lysates L2 mouse spleen lysates probed with Anti Caspase 8 Polyclonal Antibody, Unconjugated (ABIN724205) at 1:200 overnight at 4 °C. (Caspase 8 抗体 (AA 411-482))

SDS

Image provided by One World Lab validation program. (Caspase 8 抗体 (AA 411-482))

IF (cc)

6 images

(15 references)

(1 validation)

产品编号 ABIN724205

发货至: 中国

Datasheet as PDF

抗原 See all Caspase 8 (CASP8) 抗体

Caspase 8 (CASP8)
抗原表位
16

15

9

7

6

6

6

6

5

4

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 411-482
适用
132

68

61

22

15

15

10

1

1
人, 小鼠, 大鼠, 猴, Pig, 绵羊
宿主
126

23

5

2
兔
Compatible Secondaries
克隆类型
125

30
多克隆
标记
92

15

11

4

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1
This Caspase 8 antibody is un-conjugated
应用范围
138

47

44

37

26

25

24

18

18

17

6

4

4

4

1

1

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

交叉反应

人, 猴, 小鼠, Pig, 大鼠, 绵羊

预测反应

Dog,Cow,Chicken

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic peptide derived from rat CASP8 subunit p10

亚型

IgG
anti-Caspase 8 (CASP8) (AA 1-479) antibody
CASP8 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Caspase 8 (CASP8) (AA 188-280) antibody
CASP8 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Caspase 8 (CASP8) (AA 7-201) antibody
CASP8 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Caspase 8 (CASP8) (AA 385-479) antibody
CASP8 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Caspase 8 (CASP8) (AA 217-384) antibody
CASP8 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Caspase 8 (CASP8) antibody
CASP8 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 1H11 unconjugated

anti-Caspase 8 (CASP8) (AA 410-449), (N-Term) antibody
CASP8 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Caspase 8 (CASP8) (AA 217-374) antibody
CASP8 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated

anti-Caspase 8 (CASP8) (pSer347) antibody
CASP8 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated

anti-Caspase 8 (CASP8) antibody
CASP8 适用: 小鼠 WB, IHC, ELISA, FACS 宿主: 大鼠 Monoclonal 1G12 unconjugated

应用备注

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

限制

仅限研究用
生效 #029759 (Western Blotting)

by

Alamo Laboratories Inc

No.

#029759

日期

2014.07.03

抗原

Lot Number

131127

Method validated

Western Blotting

Positive Control

HeLa cells

Negative Control

c6/36 mosquito cells (non-reactive species)

Notes

A strong band was observed at the correct molecular weight in the positive control sample. No major bands were observed in the negative sample.

Validation Images

Figure 1: Western blot of lysates from HeLa cells (Lane 1), and c6/36 cells (Lane 2) probed with anti-CASP8 (upper panel) or with anti-Actin for loading control (lower panel).
Full Methods
Primary Antibody

Antigen: Caspase 8, Apoptosis-Related Cysteine Peptidase (CASP8) (1:200 dilution)
Catalog number: ABIN724205
Lot number: 131127

Secondary Antibody

Antigen: Goat Anti-Rabbit IgG (H + L)-HRP Conjugate (1:20,000 dilution)
Lot number: L170-6515

Full Protocol

1. Total protein extracts were boiled in 1X SDS Sample Buffer containing 1% SDS and 1.25% β-mercaptoethanol at 95°C for 5 min prior to loading.
2. 20 μg of boiled extracts were loaded and resolved on 8-16% SDS-polyacrylamide gel.
3. The Thermo Scientific - Spectra Multicolor Broad Range (Cat # 26634) were used as molecular mass markers.
4. Proteins were then transferred onto PVDF membrane by wet transfer and protein transfer was confirmed with Ponceau-S staining.
5. The PVDF membrane was incubated with 25 mL of blocking buffer [Tris Buffered Saline, pH 7.4 plus 0.1% TW20 (TBST)] containing 5% (W/V) BSA at room temperature for 1 h.
6. The membrane was rinsed with TBST once.
7. The membrane was immersed with the protein side up in the primary antibody solution (CASP8; 1:200) in TBST containing 5% (W/V) BSA and incubated for 16 h at 4°C.
8. The membrane was rinsed in TBST thrice for 5 min each.
9. The membrane was incubated in the HRP-conjugated secondary antibody solution (Goat anti-rabbit IgG-HRP; 1:20,000) in TBST containing 5% (W/V) BSA and incubated for 1 hour at room temperature (~26°C) with gentle agitation.
10. The membrane was rinsed thrice TBST thrice for 5 min each.
11. The membrane was rinsed in TBS twice for 30 s each.
12. Signals were detected with ECL-2 Substrate. The blot was scanned for 300 s.
13. The membrane was rinsed three times TBST.
14. Incubated in Acidic Glycine Stripping Buffer at room temperature with gentle agitation for 3 times, 10 min each.
15. The membrane was washed in TBST 2 times for 10 min each.
16. Repeated Steps 5-12 with the loading control antibody (anti-Actin; 1:6,000) and its matching secondary antibody (Goat anti-rabbit IgG-HRP; 1:20,000).

Experimental Notes

- Nothing to note.
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
Zhang, Zhao, Zhang, Hao, Yu, Min, Li, Ma, Chen, Yi, Tang, Meng, Liu, Wang, Shen, Zhang: "Decrease in male mouse fertility by hydrogen sulfide and/or ammonia can Be inheritable." in: Chemosphere, Vol. 194, pp. 147-157, (2018) (PubMed).

Tulsulkar, Glueck, Hinds, Shah: "Ginkgo biloba Extract Prevents Female Mice from Ischemic Brain Damage and the Mechanism Is Independent of the HO1/Wnt Pathway." in: Translational stroke research, Vol. 7, Issue 2, pp. 120-31, (2016) (PubMed).

Akinrinde, Oyagbemi, Omobowale, Asenuga, Ajibade: "Alterations in blood pressure, antioxidant status and caspase 8 expression in cobalt chloride-induced cardio-renal dysfunction are reversed by Ocimum gratissimum and gallic acid in Wistar rats." in: Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS), Vol. 36, pp. 27-37, (2016) (PubMed).

Gao, Liu, Li, Wu, Peng, Jing: "Hispidulin induces mitochondrial apoptosis in acute myeloid leukemia cells by targeting extracellular matrix metalloproteinase inducer." in: American journal of translational research, Vol. 8, Issue 2, pp. 1115-32, (2016) (PubMed).

Daverey, Agrawal: "Curcumin alleviates oxidative stress and mitochondrial dysfunction in astrocytes." in: Neuroscience, Vol. 333, pp. 92-103, (2016) (PubMed).

Wang, Zhao, Yu, Feng, Zhang, Kou, Chu, Cui, Li, Zhang, Shen, Min: "Regulation of steroid hormones and energy status with cysteamine and its effect on spermatogenesis." in: Toxicology and applied pharmacology, Vol. 313, pp. 149-158, (2016) (PubMed).

Zhao, Zhang, Liu, Zhang, Hao, Li, Chen, Shen, Tang, Min, Meng, Wang, Yi, Zhang: "Hydrogen Sulfide and/or Ammonia Reduces Spermatozoa Motility through AMPK/AKT Related Pathways." in: Scientific reports, Vol. 6, pp. 37884, (2016) (PubMed).

Pang, Fan: "Cytotoxic effect and possible mechanisms of Tetracaine on human corneal epithelial cells in vitro." in: International journal of ophthalmology, Vol. 9, Issue 4, pp. 497-504, (2016) (PubMed).

Yuan, Wen, Zhang, Fan: "Cytotoxicity of pilocarpine to human corneal stromal cells and its underlying cytotoxic mechanisms." in: International journal of ophthalmology, Vol. 9, Issue 4, pp. 505-11, (2016) (PubMed).

Peng, Fu, Guo, Zhang, Di, Jiang, Li: "Effects and Mechanism of Baicalin on Apoptosis of Cervical Cancer HeLa Cells I n -v itro." in: Iranian journal of pharmaceutical research : IJPR, Vol. 14, Issue 1, pp. 251-61, (2015) (PubMed).

Fang, Zhang, Qi, Fan, Luo, Liu, Tan: "Evodiamine induces G2/M arrest and apoptosis via mitochondrial and endoplasmic reticulum pathways in H446 and H1688 human small-cell lung cancer cells." in: PLoS ONE, Vol. 9, Issue 12, pp. e115204, (2014) (PubMed).

Wang, Han, Zhou, Wu, Wang, Jia, Lv, Cheng, Pan, Liu, Zhou, Sun: "Inhibition of hydrogen sulfide synthesis provides protection for severe acute pancreatitis rats via apoptosis pathway." in: Apoptosis : an international journal on programmed cell death, Vol. 18, Issue 1, pp. 28-42, (2013) (PubMed).

Wu, Tang, Jiang, Li, Jiang, Liu: "PCSK9 siRNA inhibits HUVEC apoptosis induced by ox-LDL via Bcl/Bax-caspase9-caspase3 pathway." in: Molecular and cellular biochemistry, Vol. 359, Issue 1-2, pp. 347-58, (2011) (PubMed).

Jeng, Dyson: "Direct measurement of the aspartic acid 26 pKa for reduced Escherichia coli thioredoxin by 13C NMR." in: Biochemistry, Vol. 35, Issue 1, pp. 1-6, (1996) (PubMed).
抗原

Caspase 8 (CASP8)

别名

Caspase 8 (CASP8 产品)

别名

casp8-A antibody, xCaspase-8 antibody, casp8 antibody, CASP8 antibody, CASP-8 antibody, FLICE antibody, MACH antibody, Mch5 antibody, ALPS2B antibody, CAP4 antibody, Casp-8 antibody, MCH5 antibody, caspase-8 antibody, zgc:92075 antibody, caspase 8 L homeolog antibody, death related ced-3/Nedd2-like protein antibody, caspase 8 antibody, caspase-8 antibody, caspase-8-like antibody, caspase 8, apoptosis-related cysteine peptidase antibody, casp8.L antibody, LOC661095 antibody, CASP8 antibody, LOC5563818 antibody, casp8 antibody, LOC100222284 antibody, Casp8 antibody

背景

Synonyms: MACH, Mch5, FLICE, CASP-8, Caspase-8, Casp8
Background: Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP1. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-|-AMC. Likely target for the cowpox virus CRMA death inhibitory protein.

基因ID

12370

UniProt

Q9JHX4

途径

Apoptosis, Caspase Cascade in Apoptosis, TLR signaling, Activation of Innate immune Response, Tube Formation, Positive Regulation of Endopeptidase Activity, Toll-Like Receptors Cascades