Recombinant Integrin alpha 5 beta 1 抗体
适用: 人
FACS, IP, Func, IF
宿主: 兔
Chimeric
SG-19
unconjugated
Recombinant Antibody
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- 抗原
- Integrin alpha 5 beta 1
- 抗体类型
- Recombinant Antibody
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适用
- 人
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宿主
- 兔
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克隆类型
- Chimeric
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标记
- 非结合性
- 应用范围
- Flow Cytometry (FACS), Immunoprecipitation (IP), Functional Studies (Func), Immunofluorescence (IF)
- 原理
- Anti-Integrin alpha 5 beta 1 [SG/19], Rabbit IgG, kappa
- 特异性
- This antibody binds human integrin alpha 5 beta 1 near the N-terminus. Epitope mapping of SG/19 revealed that Thr82 in the 1 subunit, located at the outer face of the boundary between the I-like and hybrid domains, was the key binding determinant for this antibody. Integrin alpha5 beta 1 is also known as fibronectin receptor and binds matrix macromolecules and proteinases and thereby stimulates angiogenesis. Integrin α5β1, is a member of the integrin family, is a heterodimer composed by two subunits, α5 (ITGA5/CD49e) and β1 (ITGB1/CD29), and both are necessary for complete biological functions. The interaction of VLA-5 with fibronectin plays an important role in regulating inflammatory cytokine production by human articular chondrocytes (From the Cell Migration Gateway ITGA5 ITGB1
- 产品特性
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Original Species of Ab: Mouse
Original Format of Ab: IgG1
- 纯化方法
- Protein A affinity purified
- 免疫原
- The original antibody was generated by immunizing BALB/c mice with Nalm-6 cells.
- 克隆位点
- SG-19
- 亚型
- IgG kappa
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- 应用备注
- This antibody was capable of recognizing integrin alpha 5 beta 1 expressed on the surface of K562 cells via immunofluorescence staining. This antibody also precipitated molecules with weight of 110 and 140 kDa under nonreducing conditions from K562 cells and Ramos cells, and 130 kDa when reduced. These results were similar to the ones reported for integrin alpha 5 beta 1. The effect of this antibody on migration beneath stromal cells was investigated by inoculating Nalm-6 and BMS2 cells together with this antibody. It was seen that the presence of this antibody blocked the migration of Nalm-6 cells beneath stromal cells (PMID: 1400595).
- 说明
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This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
- 限制
- 仅限研究用
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- 浓度
- 1 mg/mL
- 缓冲液
- PBS with 0.02 % Proclin 300.
- 储存液
- ProClin
- 注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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- 抗原
- Integrin alpha 5 beta 1
- 背景
- CD29, ITGB1, FNRB, MDF2, MSK12, ITGA5:ITGB1, Integrin alpha V beta1, Integrin beta-1, Fibronectin receptor subunit beta, Glycoprotein IIa, GPIIA, VLA-4 subunit beta
- UniProt
- P05556
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