Recombinant XCR1 抗体
-
- 抗原 See all XCR1 抗体
- XCR1 (Chemokine (C Motif) Receptor 1 (XCR1))
- 抗体类型
- Recombinant Antibody
-
适用
- 人
-
宿主
- 兔
-
克隆类型
- Chimeric
-
标记
- This XCR1 antibody is un-conjugated
-
应用范围
- ELISA, Flow Cytometry (FACS)
- 原理
- Anti-XCR1 [5G7], Rabbit IgG, kappa
- 特异性
- This antibody binds human XCR1, more particularly it strongly binds amino acids 'PESTTFFYYDLQ' of the human XCR1. XRC1 is a receptor for chemokines SCYC1 and SCYC2 and expressed on 80 % of CD8+ the dendritic cells. It also acts as a receptor for XCL1/Lymphotactin. It transduces a signal by increasing the intracellular calcium ions level.
- 产品特性
-
Original Species of Ab: Mouse
Original Format of Ab: IgG2b
- 纯化方法
- Protein A affinity purified
- 免疫原
- The original antibody was generated by immunizing XCR1 knockout mice with human XCR1-expressing B300.19 cells mixed with equal volume of GERBU adjuvant.
- 克隆位点
- 5G7
- 亚型
- IgG kappa
- Top Product
- Discover our top product XCR1 Primary Antibody
-
anti-Chemokine (C Motif) Receptor 1 (XCR1) (AA 244-322) antibody
XCR1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C Motif) Receptor 1 (XCR1) (Cytoplasmic Domain) antibodyXCR1 适用: 人, 猴 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C Motif) Receptor 1 (XCR1) (Extracellular Domain) antibodyXCR1 适用: 人 IHC, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C Motif) Receptor 1 (XCR1) (Internal Region) antibodyXCR1 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C Motif) Receptor 1 (XCR1) (AA 305-333) antibodyXCR1 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
-
- 应用备注
- This antibody is capable of binding human XCR1-EGFP-expressing B300.19 cells in a flow cytometry analysis. The determination of the binding epitope of this antibody was done using ELISA (US9371389). This antibody exhibits a significant effect in treating rheumatoid arthritis in the experiment of delayed-type hypersensitivity reaction induced by Mycobacterium butyricum delayed-type hypersensitivity reaction. The neutralizing activity of this antibody against human lymphotactin-induced migration of human XCR1-expressing cells was evaluated by in vitro chemotaxis assay. It was reported that this antibody completely inhibited cell migration at a concentration of about 3 μg/mL (US9371389). The effect of this antibody on ear swelling of DNFB (Dinitrofluorobenzene)-sensitized mice was done and it was reported that this antibody caused significant suppression of ear swelling 24 after induction. This antibody was also reported to show showed a very low reactivity to human CX3CR1-EGFP-expressing B300.19 cells (US9371389). The original antibody with saporin-conjugated secondary antibody showed growth suppression of human XCR1-EGFP-expressing B300.19 cells. The humanized version of this antibody is also available on request (US9371389).
- 说明
-
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format for improved compatibility with existing reagents assays and techniques.
- 限制
- 仅限研究用
-
- 浓度
- 1 mg/mL
- 缓冲液
- PBS with 0.02 % Proclin 300.
- 储存液
- ProClin
- 注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
-
-
