Recombinant HLA-A2 抗体
-
- 抗原
- HLA-A2
- 抗体类型
- Recombinant Antibody
- 适用
- 人
-
宿主
- 兔
-
克隆类型
- Chimeric
-
标记
- 非结合性
-
应用范围
- Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Western Blotting (WB), ELISA
- 原理
- Anti-HLA-A2 [BB7.2], Rabbit IgG, kappa
- 特异性
- This antibody binds the carboxy terminus of the α2-helix of class I MHC molecule and a turn on one of the underlying β-strands.
- 产品特性
-
Original Species of Ab: Mouse
Original Format of Ab: IgG2b
- 纯化方法
- Protein A affinity purified
- 免疫原
- The original antibody was generated by immunizing BALB/c mice with papain solubilized HLA-A2 and B7 antigens.
- 克隆位点
- BB7-2
- 亚型
- IgG kappa
-
anti-HLA-A2 antibody
适用: 人 FACS, IP 宿主: 小鼠 Monoclonal BB7-2 unconjugated
anti-HLA-A2 antibody (PE)适用: 人 FACS 宿主: 小鼠 Monoclonal BB7-2 PE
anti-HLA-A2 antibody适用: 人 FACS, IP, IHC (fro), IHC, Func, IHC (p) 宿主: 小鼠 Monoclonal BB7-2 unconjugated
anti-HLA-A2 antibody适用: 人 FACS, IP, IHC (fro) 宿主: 小鼠 Monoclonal BB7-2 unconjugated
anti-HLA-A2 antibody适用: 人 FACS, IP, IHC (fro), Func 宿主: 小鼠 Monoclonal BB7-2 unconjugated
-
- 应用备注
- The binding specificity of BB7.2 towards HLA-A2 was assessed using complement-mediated cytotoxicity and cellular radio binding assay. This antibody is cytotoxic for peripheral blood leukocytes and lymphoid cell lines. The original IgG2b version of this antibody binds HLA-A2 positive cells with a binding or avidity constant of about 5 x 108 liter/mol (PMID:7035415). This antibody was used to detect HLA-A*0201-PR1 complexes using western blot and ELISA (PMID: 17493346). This antibody was also used to detect HLA-A2 complexed with pol, gag, and EBV peptides using ELISA (PMID:8562505). This antibody was used to screen human leucocyte antigen (HLA)-A2 haplotype-positive HBV patients using flow cytometry (PMID: 16108766). This antibody was also used for the immunofluorescent staining of HLA-A2.1 Molecules on the cell surface of human processing defective cell line (T2) (PMID:7684681). This antibody was used in a study involving the expression of HLA class molecules in head and neck squamous cell carcinoma (HNSCC) specimens by immunohistochemistry (PMID: 9328140).
- 说明
-
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.
- 限制
- 仅限研究用
-
- 浓度
- 1 mg/mL
- 缓冲液
- PBS with 0.02 % Proclin 300.
- 储存液
- ProClin
- 注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
-
- 抗原
- HLA-A2
- 物质类
- Influenza Protein
- 背景
- HLA-A, Human leukocyte antigen A, HLA class I histocompatibility antigen, A-2 alpha chain, MHC class I antigen A-2 HLA-A, HLA class I histocompatibility antigen A-28 alpha chain, HLA class I histocompatibility antigen, A-28 alpha chain, MHC class I antigen A-28
- UniProt
- P04439
-
