Recombinant PDCD1 (Nivolumab Biosimilar) 抗体 (AA 1-167)
适用: 人
FACS, BR, IHC, SPR
宿主: 兔
Chimeric
5C4-B8
unconjugated
Recombinant Antibody
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- 抗原 See all PDCD1 (Nivolumab Biosimilar) products
- PDCD1 (Nivolumab Biosimilar)
- 抗体类型
- Recombinant Antibody
- 抗原表位
- AA 1-167
- 适用
- 人
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宿主
- 兔
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克隆类型
- Chimeric
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标记
- This PDCD1 (Nivolumab Biosimilar) antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Blocking Reagent (BR), Immunohistochemistry (IHC), Surface Plasmon Resonance (SPR)
- 原理
- Anti-PD-1 [5C4.B8 (Nivolumab)], Rabbit IgG, kappa
- 特异性
- Nivolumab binds to the extracellular portion of human PD-1 (2.6 nM - Scatchard analysis and SPR) - the antibody also binds to cynomolgus PD-1 with a similar affinity (3.9 nM - SPR). The antibody does not bind to other immunoglobulin superfamily proteins such as CD28, CTLA-4, ICOS and BTLA. The epitope of Nivolumab for both human and cynomolgus PD-1 includes the sequences SFVLNWYR-MSPSNQTDKLAAFPEDR (aa 29-53) and SGTYLCGAISLAPKAQIKE (aa 85-103), as shown by mass spectrometry of protease-treated fragments of PD-1 - these residues are thought to additionally be important for liand binding to PD-1. PD-1 is an inhibitory receptor expressed on the surface of T cells. It is able to bind to its ligands PDL-1 and PDL-2 which results in an inhibitory signal leading to decreased T cell proliferation, cytokine protuction and cytotoxic activtiy. PDL-1 is often expressed in human tumors such as melanoma, lung and kidney where it is able to overactivate PD-1 and plays a role in the evasion of cancer cells from the immune system.
- 交叉反应
- Cynomolgus
- 产品特性
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Original Species of Ab: Human
Original Format of Ab: IgG4
- 纯化方法
- Protein A affinity purified
- 免疫原
- mAb PD1.5 was prepared by immunizing IgH and IgK knock-out transgenic mice possessing a human immunoglobulin (heavy chain) minilocus with recombinant human PD-1-Fc protein consisting of the extracellular domain of PD-1 (amino acids 1-167) and the Fc portion of human IgG1, and Chinese hamster ovary (CHO) cells expressing human PD-1. Nivolumab was generated by grafting the variable regions of PD1.5 onto human kappa and IgG4 constant regions containing an S228P mutation (prevents Fab arm exchange with endogenous IgG4 antibodies).
- 克隆位点
- 5C4-B8
- 亚型
- IgG kappa
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- 应用备注
- Nivolumab has been shown to bind to PD-1-expressing CHO cells (EC50 ~1.66nM). Nivolumab binds CD4+ T cells (EC50 ~0.64 nM) and stains only memory and effector, and not naiive CD4+ or CD8+ T cells from human peripheral blood by FC. The antibody is able to block the interaction between PD-1 and its ligands PDL-1 and PDL-2 (IC50 ~2.52 nM and ~2.59 nM, respectively - determined by SPR) - these IC50 values are also similar to that measured by FACS to evaluate ligand binding to PD-1 expressed on CHO cells. In an allogenic T-cell/DC MLR, Nivolumab-mediated inhibition of PD-1 results in enhancement of IFNγ release, and also enhances IL-2 secretion (97-139 % over an isotype control) in response to the superantigen SEB using human peripheral blood mononuclear cells. The same is also observed in a CMV-restimulation assay. Nivolumab at very low concentrations (~1.5 ng/mL) is able to enhance T-cell reactivity in the presence of a T-cell receptor stimulus - nivolumab has no stimulatory effect in the absence of antigen or T-cell receptor stimulus. In the therapeutically used human IgG4 (S228P) format, this antibody is unable to mediate ADCC (antibody-dependent cell-mediated cytotoxicity) or CDC (complement-dependent cytotoxicity).
- 说明
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This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG4 format, for improved compatibility with existing reagents, assays and techniques.
- 限制
- 仅限研究用
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- 浓度
- 1 mg/mL
- 缓冲液
- PBS with 0.02 % Proclin 300.
- 储存液
- ProClin
- 注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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- 抗原
- PDCD1 (Nivolumab Biosimilar)
- Abstract
- PDCD1 (Nivolumab Biosimilar) 产品
- 物质类
- Biosimilar
- 背景
- CD279, programmed death-1, programmed death 1 , PD1, PD 1, Programmed cell death protein 1, hPD-1, hPD1, hPD 1, BMS-936558, MDX-1106,ONO-4538
- UniProt
- Q15116
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