电话:
+86 (0512) 65829739
传真:
+86 (010) 6788 5057
电子邮件:
orders@antibodies-online.cn

Nuclease 抗体

Rockland 200-401-mu6 适用: Serratia marcescens ELISA, WB 宿主: 兔 Polyclonal unconjugated
Rockland
产品编号 ABIN7540666
Supplier Product No.: 200-401-mu6
发货至: 中国
  • 抗原 See all Nuclease products
    Nuclease
    适用
    Serratia marcescens
    宿主
    • 4
    克隆类型
    • 4
    多克隆
    标记
    • 2
    • 2
    This Nuclease antibody is un-conjugated
    应用范围
    • 4
    • 4
    • 3
    • 1
    • 1
    ELISA, Western Blotting (WB)
    Supplier Product No.
    200-401-mu6
    Supplier
    Rockland
    原理
    Nuclease/NucA Antibody
    交叉反应 (详细)
    This antibody will react with endonuclease from S.
    纯化方法
    This antibody was purified from monospecific antiserum by Protein A chromatography.
    过滤
    Sterile filtered
    免疫原
    Anti-Nuclease/NucA was prepared by repeated immunizations with recombinant benzonase protein produced in E. coli.
    亚型
    IgG
  • 应用备注

    ELISA_Dilution: 1:21,600 - 1:22,600

    Western_Blot_Dilution: 1:1000

    说明

    Anti-Nuclease/NucA has been tested by ELISA and western blot. Expect a band ~ 28 kDa in size corresponding to endonuclease by western blotting in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.

    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    有效期
    12 months
  • 抗原
    Nuclease
    别名
    nucA (Nuclease 产品)
    背景
    Benzonase, Nuclease, Endonuclease, Serratia marcescens endonuclease, NucA,Nucleases are secreted by Serratia marcescens into the medium it surrounds. The enzyme is a sugar-nonspecific hydrolase, capable of cleaving both RNA and DNA in either double or single stranded form. It requires divalent cations, preferably Mg2+, and is functional across a broad pH range from 6 to 10 (optimal at 8-8.5) and wide temperature ranges between 35 °C and 44 °C. The ability of Serratia to secrete nuclease appears to be regulated. Bacterial cultures at differing cell densities display different kinetics and efficiencies of nuclease secretion [i.e. growth medium, growth conditions, and host cell mutations]. Anti-Nuclease/NucA Antibody is useful for researcher interested in identifying nucleic acid contamination.
    UniProt
    P13717
You are here:
客服