This antibody is expressed in HEK293 cells and and was purified using a combination of Protein A/G chromatography. It is reactive against the EV membrane protein of monkeypox virus (Homologous to Vaccinia virus B5R). It also reacts to VACV antigen and lysate, CPXV lysate, MPXV lysate, and VARV antigen and lysate. MPXV-13 weakly binds to VACV purified antigen, VACV and MPXV virus-infected cell lysates, and strongly binds VARV purified antigen and CPXV virus-infected cell lysate. This product is sold for research use only. Optimal working concentrations should be determined by each researcher.
纯化方法
This human recombinant IgG1 antibody is purified using a multiple step process to include Protein A or Protein G chromatography. It is produced using in vitro cell culture techniques. Antibody concentration was determined using an absorbance at 280 nm: 1.4 equals 1.0 mg of IgG.
纯度
Purified Protein
克隆位点
1E2
亚型
IgG1
应用备注
This antibody reacts with monkeypox virus as determined by ELISA. Optimal working dilutions should be determined experimentally by the investigator.
限制
仅限研究用
状态
Liquid
缓冲液
PBS, pH 7.2, 0.09 % Sodium azide
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
4 °C,-80 °C
储存方法
Antibody may be stored between 2°C and 8°C for up to 30 days. For long-term storage, aliquot and store at -80°C. Multiple freeze-thaw cycles are not recommended.