Immunohistochemistry on frozen sections: Suggested dilution: 1:20-1:40 for 60 minutes at 25°C. This is provided as a guide and users should determine their own optimal working dilutions. NCL-Hamlet requires the sections to be fixed in acetone/ methanol at a ratio of 1:1 for 4 minutes at 25°C prior to incubation with the primary antibody. Immunohistochemistry on paraffin sections. Suggested dilution: 1:20-1:40 for 60 minutes at 25°C. High temperature antigen retrieval using 0.01 M citrate retrieval solution ( pH 6.0) is recommended. This is provided as a guide and users should determine their own optimal working dilutions.,Immunohistochemical (IHC) staining techniques allow for the visualization of antigens via the sequential application of a specific antibody to the antigen (primary antibody), a secondary antibody to the primary antibody and an enzyme complex with a chromogenic substrate with interposed washing steps. The enzymatic activation of the chromogen results in a visible reaction product at the antigen site. The specimen may then be counterstained and coverslipped. Results are interpreted using a light microscope.
限制
仅限研究用
状态
Lyophilized
溶解方式
The user is required to reconstitute the contents of the vial with the correct volume of sterile distilled water as indicated on the vial label.
浓度
180 mg/L
缓冲液
This product is a lyophilized tissue culture supernatant containing sodium azide as a preservative.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
4 °C
储存方法
Store at 2-8 °C. Do not freeze. Return to 2-8 °C immediately after use. Do not use after expiration date indicated on the vial label. Storage conditions other than those specified above must be verified by the user.