This antibody reacts with full-size calpain 3 (94 kD) plus an additional breakdown product at 60 kD in human skeletal muscle. The 94 kD band can be seen in muscle extracts from rabbit, mouse, dog, chicken, hamster, pig and rat. Degraded calpain 3 bands starting at approximately 60 kD are also usually present. Additional bands corresponding in size to calpains 1 and/or 2 can be detected in skeletal muscle from mouse, rat, chicken and hamster.
纯化方法
Tissue culture supernatant
免疫原
Synthetic peptide containing amino acids 355-370 of the human calpain 3 sequence.
Western Blotting: Typical working dilution 1:100. The optimal dilution should be determined by the user as calpain must be homogenized in treatment buffer
限制
仅限研究用
状态
Lyophilized
溶解方式
Reconstitute with the volume of sterile distilled water indicated on the vial label.
缓冲液
Lyophilized tissue culture supernatant containing 15 mM sodium azide.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
4 °C
储存方法
Store at 2-8 °C. Do not freeze. Return to 2-8 °C immediately after use. Do not use after expiration date indicated on the vial label. Storage conditions other than those specified above must be verified by the user.