BIP/GRP78 抗体
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- 抗原 See all BIP/GRP78 抗体
- BIP/GRP78 (Luminal-Binding Protein (BIP/GRP78))
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适用
- 人
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宿主
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绵羊
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克隆类型
- 多克隆
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标记
- This BIP/GRP78 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunodepletion (IDe), Immunoelectrophoresis (IEP), Neutralization (Neut)
- 原理
- GRP78 Polyclonal Antibody
- 特异性
- Human GRP78 (HSPA5) (Heat Shock 70 kDa Protein 5 (Glucose-Regulated Protein, 78 kDa)
- 纯化方法
- Antigen-specific affinity purification
- 免疫原
- Recombinant human GRP78
- 亚型
- IgG
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- 应用备注
- Immunoblot 0.5-5 μg/mL, ELISA capture 10 μg/mL
- 说明
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Affinity-purified IgG are antibodies further purified using immobilized antigen. Affinity-purification is an enrichment process in which whole IgG is passed over a resin to which antigen has been covalently attached. Non-reactive antibodies are washed away and specific antibodies are eluted under conditions that disrupt the antigen-antibody interaction. The result is a very high-purity product with a substantially higher titre than whole IgG. This level of product is intended for applications such as immunoblotting, immunostaining of cells and several types of immunoassays where the higher signal-to-noise ratio achieved with this enriched product is required.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 溶解方式
- Allow product to warm to room temperature and gently mix before use
- 浓度
- 1 mg/mL
- 缓冲液
- 10 mM HEPES, pH 7.4, 150 mM NaC, 50 % (v/v) glycerol
- 储存条件
- -20 °C
- 储存方法
- Store between -10°C and -20°C
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- 抗原
- BIP/GRP78 (Luminal-Binding Protein (BIP/GRP78))
- 别名
- Glucose-Regulated Protein 78 (GRP78) (BIP/GRP78 产品)
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