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CD77 抗体 (FITC)

适用: 人 FACS 宿主: 大鼠 Monoclonal 38-13 FITC
产品编号 ABIN7505953
发货至: 中国
  • 抗原 See all CD77 products
    CD77
    适用
    宿主
    • 5
    大鼠
    克隆类型
    • 5
    单克隆
    标记
    • 2
    • 2
    • 1
    This CD77 antibody is conjugated to FITC
    应用范围
    • 5
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS)
    原理
    Anti-Hu CD77 FITC
    特异性
    The rat monoclonal antibody 38.13 recognizes CD77 (globotriaosylceramide Gb3), a neutral glycosphingolipid expressed mainly on the surface of B cell populations, such as germinal center B cells and Burkitt´s lymphoma cells.
    纯化方法
    Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
    免疫原
    Daudi cell line (Burkitt´s lymphoma)
    克隆位点
    38-13
    亚型
    IgM
  • 应用备注
    Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μL reagent / 100 μL of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
    限制
    仅限研究用
  • 缓冲液
    Stabilizing Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C
    储存方法
    Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
  • 抗原
    CD77
    Abstract
    CD77 产品
    背景
    CD77 (globotriaosylceramide Gb3), also known as the Pk blood group antigen, BLA (Burkitt´s lymphoma associated antigen), or CTH (ceramide trihexoside) is a neutral glycosphingolipid composed of three carbohydrate molecules linked to a lipid moiety in the cell membrane (Gal-alpha1-4Gal-beta1-4Glc-beta1-Cer). It is expressed on germinal center B cells, Burkitt´s lymphoma cells, it can be induced on extrafolicular B cells and it is also found on endothelia and epithelia. CD77 may be involved in elimination of germinal center B cells that fail to produce high affinity antibodies, and serves also as receptor for shiga toxin and verotoxin.,globotriaosylceramide Gb3, Gal-alpha1-4Gal-beta1-4Glc-beta1-Cer, P blood group
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