Recombinant EGFR (Matuzumab Biosimilar) 抗体
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- 抗原 See all EGFR (Matuzumab Biosimilar) products
- EGFR (Matuzumab Biosimilar)
- 抗体类型
- Recombinant Antibody
- 适用
- 人
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宿主
- 小鼠
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克隆类型
- Chimeric
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标记
- This EGFR (Matuzumab Biosimilar) antibody is un-conjugated
- 应用范围
- Blocking Reagent (BR), Flow Cytometry (FACS), Western Blotting (WB), ELISA, Antagonist (Anta), Immunofluorescence (IF)
- 特异性
- This antibody binds the extracellular domain III of the human EGFR and does not cross react with murine EGFR. It was also reported that residues S460/G461 in EGFR domain III are essential components of the epitope. This antibody binds at a site distinct from the EGF binding site, preventing conformational re-arrangement required for dimerization, a process essential for high-affinitiy ligand binding (Schmiedel, 2008). This antibody can only recognize the active form of EGFR. The antibody binds to both glyco- and aglyco receptor forms. EGFR is a transmembrane mitogenic glycoprotein with a cytoplasmically facing tyrosine kinase site which is activated by binding of the epidermal growth factor in the external domain. It also binds transforming growth factor alpha (TGFa). EGFR a member of the epidermal growth factor family and is closely related to receptor tyrosine kinases. This receptor tyrosine kinase binds ligands of the EGF family and activates signallying cascades to convert extracellular cues into appropriate cellular responses. Mutations that lead to EGFR overexpression (known as upregulation) or overactivity have been associated with a number of cancers, including lung cancer, anal cancers and glioblastoma multiforme as well as inflammatory diseases
- 产品特性
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OriginalSpeciesName: Human
OriginalFormat: IgG1
- 纯化方法
- Purified antibody.
- 免疫原
- The parental mouse antibody was generated by immunizing BALB/c mice intraperitoneally with A431 cells in phosphate buffered saline (PBS). Later on the humanzied version of the antibody was created by grafting CDRs of the murine antibody onto human constant regions.
- 亚型
- IgG1 kappa
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anti-EGFR (Matuzumab Biosimilar) (Extracellular Domain) antibody
适用: 人 BR, FACS, WB 宿主: Human Monoclonal unconjugated Recombinant Antibody
anti-EGFR (Matuzumab Biosimilar) (Extracellular Domain) antibody适用: 人 BR, FACS, WB 宿主: 兔 Chimeric unconjugated Recombinant Antibody
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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This chimeric murine antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.
- 限制
- 仅限研究用
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- 缓冲液
- PBS with 0.02 % Proclin 300.
- 储存液
- ProClin
- 注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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- 抗原
- EGFR (Matuzumab Biosimilar)
- Abstract
- EGFR (Matuzumab Biosimilar) 产品
- 背景
- ErbB-1, ERBB1, HER1, EGF, Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, EMD72000, mAb425, 425, mAb 425
- UniProt
- P00533
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