Western Blotting (WB), Immunohistochemistry (IHC), Enzyme Immunoassay (EIA)
特异性
Purified virions
交叉反应 (详细)
Specific to H1N1 by IHA. Does not react Infl. B, RSV, Para 1-3 or Adeno. UNINFECTED CELL REACTIVITY: Does not react with HEp-2 cells. May react with chicken cellular proteins.
纯化方法
This product consists of purified IgG fraction of the above antiserum covalently coupled to a highly purified preparation of horseradish peroxidase (RZ >3) by methods. Care is taken to ensure adequate conjugation while preserving maximum enzyme activity. Free enzyme is absent. Immunoglobulin concentration in this product is 1-2 mg/ml. Estimated molar HRP:IgG substitution is 2-3.
TITER : >1:1,000 by indirect immunofluorescence. >1:2,500 by hemagglutination inhibition. This product is suitable for immunohistochemical, immunoblotting and EIA applications. Working strength must be determined by the user but a starting range of 1:20 - 1:200 is recommended for immunochemistry and blotting while 1:200 - 1:1,000 is recommended for enzyme immunoassays. Functions in formalin fixed/paraffin embedded setting, IFA, ELISA, FACS & WB.
限制
仅限研究用
状态
Liquid
浓度
Lot specific
缓冲液
This preparation is supplied in a phosphate saline buffer stabilized with bovine serum albumin (10 mg/mL) and preserved with 0.002 % thimerosal. NOTE: sodium azide is a potent inhibitor of peroxidase !
储存液
Thimerosal (Merthiolate)
注意事项
This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
储存条件
4 °C,-20 °C
储存方法
Recommended short term (<6 months) storage is liquid at 2-8°C. For longer term storage, aliquot and freeze.