E. Coli 抗体 (FITC)
适用: E. coli
Func
宿主: 山羊
Polyclonal
FITC
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- 抗原 See all E. Coli products
- E. Coli
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适用
- E. coli
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This E. Coli antibody is conjugated to FITC
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应用范围
- Functional Studies (Func)
- 特异性
- Many "O" and "K" antigenic serotypes of Escherichia coli
- 交叉反应 （详细）
- Antiserum is unabsorbed and does crossreact with related Enterobacteriaceae
- 纯化方法
- This product consists of purified IgG fraction of the neat antiserum covalently coupled with high purity Isomer I of fluorescein isothiocyanate. Care is taken to ensure complete removal of any free fluorescein from the final product.
- 免疫原
- Mixture of E. coli serotypes
- 亚型
- IgG
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- 应用备注
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TITER : > 1:1,600 by indirect IFA vs serotypes O157:H7, O20, O125, O55, O111 & K12
Applications for this product include direct FA staining of target antigens in a permissive tissue culture system. Working dilutlion must be determined by the user for his or her application but a starting range of 1:10 - 1:50 is suggested. Acetone fixation of the antigen source is recommended prior to staining. We also recommend use of COUNTERSTAIN/BLOCKING DILUENT (Cat.#) and MOUNTING MEDIUM (Cat.#) for optimum product performance. Enzyme amplification following reaction with FITC conjugate can also be accomplished utilizing enzyme-antibody conjugates specific to FITC (Cat.#s & ). - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- The product is formulated in a phosphate saline buffer (0.01M, pH 7.2) containing bovine serum albumin (10 mg/mL) as a stabilizer and 0.1 % sodium azide preservative.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Recommened short term (<6 months) storage is liquid at 2-8°C under subdued light. For longer term storage, aliquot and freeze.
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- 抗原
- E. Coli
- Abstract
- E. Coli 产品
- 物质类
- Bacteria
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