E. Coli 抗体 (Biotin)
适用: E. coli
ELISA, WB, IHC, FM
宿主: 兔
Polyclonal
Biotin
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- 抗原 See all E. Coli products
- E. Coli
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适用
- E. coli
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This E. Coli antibody is conjugated to Biotin
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应用范围
- ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Fluorescence Microscopy (FM)
- 特异性
- All "O" and "K" antigenic serotypes of Escherichia coli
- 交叉反应 （详细）
- Antiserum is unabsorbed and does crossreact with related Enterobacteriaceae
- 纯化方法
- This product consists of purified IgG fraction of neat antiserum covalently coupled with the N-Hydroxysuccinimide ester of biotin under mild conditions to give a high degree of substitution.
- 免疫原
- Mixtures of all antigenic serotypes
- 亚型
- IgG
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- 应用备注
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TITER : > 1:2,000 by ELISA vs serotypes O157:H7, O20, O125, O55, O111 & K12
Possible applications for this product include avidin and streptavidin amplification systems for immunohistochemistry, ELISA, fluorescence microscopy and immunoblotting. In addition, this product may be used in place of neat antiserum in almost any appropriate antibody-based technique. Will remove E. coli proteins from recombinant preparations - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- The product is formulated in a phosphate saline buffer (0.01M, pH 7.2) containing 0.1 % sodium azide preservative. No stabilizing proteins have been added.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Recommended short term (<6 months) storage is liquid at 2-8°C. For longer term storage, aliquot and freeze.
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- 抗原
- E. Coli
- Abstract
- E. Coli 产品
- 物质类
- Bacteria
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